اثر تمرین تناوبی با شدت بالا و مکمل یاری کورکومین بر شاخص‌های آپوپتوزیس TNF-α،nfkb و fas بر سمیت عصبی هیپوکمپ ناشی از مصرف آب اکسیژنه در رت های نر

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی گرایش عصب و عضله، گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران

2 گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد واحد بجنورد، بجنورد، ایران

3 گروه تربیت بدنی و علوم ورزشی دانشگاه آزاد واحد بجنورد، بجنورد ایران

10.22049/jahssp.2023.28273.1543

چکیده

هدف: آپوپتوز نوعی مرگ برنامه­ریزی شده است در تنظیم تعادل بین مرگ سلولی و رشد بافت نقش مهمی دارد. بنابراین هدف پژوهش حاضر، بررسی اثر تمرین تناوبی با شدت بالا و مکمل یاری کورکومین بر شاخص­های آپوپتوز فاکتور نکروز تومور آلفا، فاکتور هسته‌ای تقویت‌کننده زنجیره سبک کاپا و فاس بر سمیت عصبی هیپوکمپ ناشی از مصرف آب اکسیژنه در رت­های نر می­باشد. روش شناسی: این تحقیق از نوع تحربی بوده که در آن 50 سر رت نر بالغ از نژاد ویستار با وزن 20±200 گرم و سن 8 هفته به طور تصادفی به 5 گروه مساوی 1) سالین، 2) آب اکسیژنه (H2O2)،  3) تمرین تناوبی شدید+ H2O2، 4) مکمل کورکومین+ H2O2، 5) تمرین تناوبی شدید+مکمل کورکومین+ آب اکسیژنه ( 10n=) تقسیم شدند.   H2O2 مقدار 1 میلی­مول به ازای هر کیلوگرم از وزن 3 بار در هفته در روزهای زوج و کورکومین، روزانه 150 میلی­گرم به ازای هر کیلوگرم وزن بدن استفاده شد. برنامه تمرین دویدن بر روی تردمیل 8 هفته (پنج جلسه در هفته با شدت 85-90 درصد سرعت بیشینه) انجام شد. بافت هیپوکامپ رت­ها در پایان دوره تحقیق برای ارزیابی تغییرات بیان ژنی متغیرهای مورد بررسی استخراج شد. داده­ها با استفاده از روش آنالیز واریانس یک طرفه در سطح معناداری 05/0≥p تحلیل شد. یافته‌ها: یافته­ها نشان داد تزریق آب اکسیژنه منجر به افزایش معنادار بیان ژن­های TNF-α، NF-κB و  Fas(0001/0P=) شده است. تمرین HIIT و مکمل­یاری کورکومین به تنهایی و به صورت ترکیبی به­ ترتیب منجر به تغییرات کاهشی نسبت به گروه آب اکسیژنه در بیان ژن­های TNF-α، NF-κB  و Fas داشتند (0001/0P=) که نشان­دهنده اثر مثبت تمرین ورزشی و مکمل­یاری کورکومین بر شاخص­های مورد نظر می­باشد. نتیجه‌گیری: چنین به نظر می­رسد احتمالا" تزریق برون­زاد H2O2 منجر به افزایش روند آپوپتوز نورونی می­شود. انجام تمرینات HIIT و مکمل کورکومین توانست فرآیند آپوپتوز را کاهش دهد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of high-intensity interval training and curcumin supplementation on apoptosis indicators such as TNF-α, nfkb and fas on hippocampal neurotoxicity caused by hydrogen peroxide consumption in male rats

نویسندگان [English]

  • Zahra Toktam Barmar 1
  • Sadegh Cheragh-Birjandi 2
  • Najmeh Rezaeian 3
1 Doctoral student of sports physiology majoring in nerve and muscle, Department of Physical Education and Sports Sciences, Bojnord Branch, Islamic Azad University, Bojnord, Iran
2 Department of Physical Education, Isalmic Azad University, Bojnourd branch, Bojnourd, Iran
3 Department of Physical Education Islamic Azad University, Bojnourd Branch, Bojnourd, Iran
چکیده [English]

Aim:   Apoptosis is a type of programmed death that is important in regulating the balance between cell death and tissue growth. Thus, the aim of this study is to investigate the effect of high-intensity interval training and curcumin supplement on apoptosis indicators such as TNF-α, NF-kB and Fas on hippocampal neurotoxicity caused by hydrogen peroxide consumption in male rats. Methods: This is an experimental research in which 50 adult male Wistar rats weighing 20 ± 200 g and aged 8 weeks were randomly divided into 5 equal groups of saline, hydrogen peroxide (H2O2, intense intermittent exercise + oxygenated water, curcumin supplement + hydrogen peroxide and intense intermittent exercise + Curcumin supplementation + hydrogen peroxide (n=10) were divided. H2O2 in the amount of 1 mmol per kg of body weight 3 times a week on even days and curcumin, 150 mg per kg of body weight per day, was used by gavage. The treadmill running program was performed for 8 weeks (five sessions per week with an intensity of 85-90 % of the maximum speed). At the end of the injection and training period, rats were extracted to evaluate changes in gene expression of hippocampal tissue. Data were analyzed using one-way ANOVA method at a significant level (α≥0.05). Results:  The findings showed that hydrogen peroxide injection led to a significant increase in the expression of TNF-α, NF-κB, Fas genes (P=0.001). HIIT exercise and curcumin supplement each alone and together lead to decreasing changes in the expression of TNF-α, NF-κB, Fas (P=0.001), which shows the positive effect of exercise training and curcumin supplementation on the desired indicators.  Conclusions: It seems so, exogenous injection of H2O2 has led to an increased the process of neuronal apoptosis. HIT exercises and curcumin supplement has been able to reduce the process of apoptosis.

کلیدواژه‌ها [English]

  • apoptosis
  • high intensity intermittent exercise
  • hydrogen peroxide
  • neurotoxicity

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 10، شماره 2
مهر 1402
صفحه 54-67

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سابقه مقاله

  • تاریخ دریافت: 05 اسفند 1401
  • تاریخ بازنگری: 09 اردیبهشت 1402
  • تاریخ پذیرش: 10 اردیبهشت 1402

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