اثرشش هفته تمرینات استقامتی، مقاومتی و ترکیبی بر آنزیم‌های کبدی( ALT و AST ) در رت‌های اوریکتومی شده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزولوژی ورزشی، دانشکده تربیت بدنی، دانشگاه تهران، تهران، ایران

2 کارشناس ارشد فیزیولوژی ورزش، گروه تربیت بدنی و علوم ورزشی ، دانشگاه آزاد اسلامی واحد شبستر ، شبستر، ایران

چکیده

 هدف: بیماری کبد چرب، نوعی تجمع چربی در سلول های کبدی است که در صورت عدم کنترل،روندی بدخیم را به سمت فیبروز شدن بافت کبد و تخریب سلولی آن طی می کند. در این مطالعه تأثیر تمرینات استقامتی، مقاومتی و بر آنزیم‌های کبدی در رت‌های اوریکتومی شده مورد بررسی قرار گرفت. روش شناسی: بدین منظور50 سر موش صحرایی ماده نژاد ویستار تحت شرایط کنترل شده )دما، چرخۀ روشنایی و تاریکی و دسترسی آزاد به آب و غذا) نگهداری شدند و بعد از آشناسازی با پروتکل تمرینی، به صورت تصادفی به  5 گروه 10 تایی شامل: گروه شم، گروه اواریکتومی ( (OVX ، گروه اواریکتومی شده و تمرین مقاومتی  (OVX+RE)  ، گروه اواریکتومی شده و تمرین استقامتی (OVX+EN)، گروه اواریکتومی شده و ترکیب تمرینات استقامتی-مقاومتی (OVX+EN+RE)  تقسیم شدند. 48 ساعت پس از پایان آخرین جلسۀ تمرینی حیوانات بیهوش و تشریح شدند وبافت کبد برای بررسی آنزیم های مربوطه از بدن جدا شد. تحلیل آماری داده‌ها با استفاده از تحلیل واریانس یک طرفه و آزمون تعقیبی توکی انجام شد (05/0 p <). یافته‌ها: نتایج نشان داد که تمرینات استقامتی و مقاومتی وترکیبی بر آنزیم‌های کبدی ALT و AST در رت‌های اوریکتومی شده تأثیر معناداری دارد (05/0 p <). ولی تمرینات ترکیبی  بیشترین تاثیر را برآنزیم کبدی ALT و AST  در رت‌های اوریکتومی داشت (05/0 p <). اواریکتومی باعث افزایش آنزیم‌های کبدی ALT و AST  شد (05/0 p <). شش هفته تمرینات استقامتی و مقاومتی  به تنهایی و به صورت ترکیبی توانست آنزیم های کبدی ALT و AST  بهبود بخشد (05/0 p <) ولی اثر ترکیبی این دو مداخله قویتر بود (05/0 p <). نتیجه‌گیری: نتایج پژوهش حاضرنشان داد که ترکیب تمرینات استقامتی-مقاومتی دارای قدرت محافظت بیشتری نسبت به هر یک به تنهایی در تغییرات درارتباط با یائسگی در بافت کبد دارد. بنابراین احتمالا بهبود روش زندگی از طریق ترکیب تمرینات استقامتی-مقاومتی مناسب می‌تواند از بروز خیلی اختلالات احتمالی در دوران یائسگی جلوگیری نماید.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Six Weeks Endurance, Resistance and Combined Training on Liver ALT and AST in The Liver of Ovariectomized Rat

نویسندگان [English]

  • Morteza Jourkesh 1
  • Habib Ebadi 2
1 PhD Student of Exercise Physiology, Department of Sport Physiology, Faculty of Physical Education, University of Tehran, Tehran, Iran
2 MSc. of Exercise Physiology, Department of Physical Education and Sport Sciences, Islamic Of Azad University, Shabestar Shabestar, Iran
چکیده [English]

Aim: Fatty liver disease is a type of fatty accumulation in liver cells that, if not controlled, leads to a malignant process of fibrosis of the liver and its cellular destruction. In this study, the effects of endurance, resistance and combination exercises on liver enzymes in ovariectomized rats were investigated. Methods: For this purpose, 50 Wistar rats were kept under controlled conditions (temperature, light and dark cycle and free access to food and water) and after randomization with exercise protocol, randomly divided into 5 groups of 10 per group. : Sham, ovariectomy (OVX), ovariectomized and resistance training group (OVX + RE), ovariectomized and endurance training (OVX + EN), ovariectomy and combined endurance-resistance training (OVX + EN) + RE) .48 hours after the end of the last session rats were anesthetized and sacrificed.Statistical analysis was performed using one-way ANOVA and Tukey post hoc test (p <0.05). Results:The results showed that endurance and resistance training and combination had significant effect on ALT and AST liver enzymes in uricectomized rats (p <0.05). But combined exercise had the most effect on ALT and AST hepatic enzyme in uricectomized rats (p <0.05). Ovariectomy increased ALT and AST liver enzymes (p <0.05).Six weeks of endurance and resistance training alone and in combination improved ALT and AST hepatic enzymes (p <0.05) but the combined effect of these two interventions was stronger (p <0.05). Conclusions: The results of the present study showed that the combination of endurance-resistance training has more protective power than any alone in changes in menopause in liver tissue. Therefore, possibly improving lifestyle by combining appropriate endurance-resistance exercises can prevent many potential disorders during menopause.

کلیدواژه‌ها [English]

  • Exercise training
  • Ovariectomy
  • AST
  • ALT
  • Liver
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