مقایسه تاثیر10 هفته تمرین تناوبی با شدت بالا باتمرین استقامتی تداومی بر بیان میر205 و ژن VEGFدرموش‌های مبتلا به سرطان پستان

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد، گروه فیزیولوژِی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز،ایران.

2 دانشیار فیزیولوژی ورزشی، گروه فیزیولوژِی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز،ایران.

چکیده

در این پژوهش، مقایسه تاثیر 10 هفته تمرین تناوبی با شدت بالا با تمرین استقامتی تداومی بربیان MIR-205 و ژن VEGF در موش های مبتلا به سرطان پستان مورد بررسی قرار گرفته است. مواد و روش‌ها: مطالعه برروی23 سر موش بالب سی ماده 6تا7هفته ای،با میانگین توده بدنی20-18گرم انجام شد. رده سلولی کارسینومای وابسته به استروژن MC4-L2 درمحیط کشت،کشت داده شد، و سپس تعداد یک میلیون سلول سرطانی به شکل زیرپوستی به هرموش تزریق شد.یک هفته پس ازایجاد تومور،موش‌ها به شکل تصادفی به سه گروه 6 تایی: کنترل(C)، هوازی تناوبی(E)،تناوبی باشدت بالا(H)، تقسیم شدند.تمرینات به مدت 10 هفته،5 بار در هفته انجام شد.هرجلسه پروتکل گروه E باشدت65-60درصد vo2max وگروه H با شدت 90-85 درصد vo2maxبود. میزان بیانMIR-205 وژن VEGF به روش Real time-PCR اندازه گیری شد.یافته‌ها: میزان بیان MIR-205 در گروه تناوبی باشدت بالا (000/0=p)، و در گروه استقامتی تداومی(001/0=p)،نسبت به گروه کنترل افزایش معناداری داشت. و میزان بیان ژنVEGF در گروه تناوبی باشدت بالا(003/0=p)، و درگروه استقامتی هوازی (005/0=p)، نسبت به گروه کنترل کاهش معناداری داشت. نتیجه‌گیری: باتوجه به اثر افزایشی تمرین تناوبی باشدت بالا واستقامتی هوازی بربیان MIR-205 و اثر کاهشی بربیان ژن VEGF در موش‌های مبتلا به سرطان پستان،این دو نوع تمرین باعث کاهش رشد تومور شدند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of ten weeks of high intensity interval training and continuous endurance training on the expression of MIR-205 and VEGF gene in mice with breast cancer has been compared

نویسندگان [English]

  • ziba rezaei 1
  • Saeid shakerian 2
  • Masoud Nikbakht 2
1 M.Sc., Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 Associate Professor of Sport Physiology, Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
چکیده [English]

the effect of ten weeks of high intensity interval training and continuous endurance training on the expression of MIR-205 and VEGF gene in mice with breast cancer has been compared.
Materials and Methods: Study was done on 23 female Balb/crats with age of6 to 7 weeks and mean body mass of 20-18 g. the estrogen-dependent carcinoma cell line MC4-L2 were cultured in the culture medium. Then one million cancer cells were injected into each mouse subcutaneously. A week after tumor induction, mice were randomly divided into three groups of 6: control (C), continuous aerobic (E), interval high intensity (H). Training were done for 10 weeks, five times a week. Each session of protocol for Group E was done with the intensity of 65-60 percent vo2max and for Group H was done with an intensity of 90-85 percent vo2max . Expression levels of MIR-205 and VEGF genes were measured by Real time-PCR metho. Results: compared to the control group ,MIR-205 expression levels in high intensity Interval (p=0/000), and continuous endurance (p=0/001), increased significantly. And compared to control group, gene expression levels of VEGF in high intensity Interval (p=0/003), and aerobic endurance group (p=0/005), decreased significantly. Conclusion: with regard to the increasing effect of High intensity aerobic Interval training and aerobic endurance training on the expression of MIR-205 and depressing effect of these mentioned training on VEGF gene expression, these two types of practice could reduce tumor growth on mice with breast cancer.

کلیدواژه‌ها [English]

  • breast cancer
  • continuous endurance training
  • Hiit training
  • mir-205
  • VEGF
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