مقایسه تاثیر10 هفته تمرین تناوبی با شدت بالا باتمرین استقامتی تداومی بر بیان میر205 و ژن VEGFدرموش‌های مبتلا به سرطان پستان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد فیزیولوژی ورزشی، گروه فیزیولوژِی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز،ایران

2 دانشیار فیزیولوژی ورزشی، گروه فیزیولوژِی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز،ایران

3 دانشیار فیزیولوژی ورزشی، گروه فیزیولوژِی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز،ایران.

چکیده

  هدف: ژن‌های متعددی در ایجاد سرطان پستان شرکت دارد. یکی از آن­ها  ژنVEGF (فاکتور رشد آندوتلیال عروقی) که از مهم­ترین فاکتورهای آنژیوژنز است. از طرفی یکی ازمیکرو rna های سرکوب‌گر MIR-205 است که به طور قابل توجهی در تومورهای پستان در مقایسه با بافت سالم همسان کمتر ترجمه (بیان ) می شود. بنابراین در این پژوهش، مقایسه تأثیر 10 هفته تمرین تناوبی با شدت بالا با تمرین استقامتی تداومی بر بیان MIR-205 و ژن VEGF در موش‌های مبتلا به سرطان پستان مورد بررسی قرار گرفته است. روش شناسی: مطالعه روی 18 سر موش بالبسی ماده 6  تا 8 هفته‌ای، با میانگین توده بدنی20-18 گرم انجام شد. رده سلولی کارسینومای وابسته به استروژن MC4-L2 درمحیط کشت،کشت داده شد. سپس تعداد یک میلیون سلول سرطانی به شکل زیر پوستی به هر موش تزریق شد. یک هفته پس از ایجاد تومور، موش‌ها به شکل تصادفی به سه گروه 6 تایی: کنترل (C)، استقامتی تداومی (E)، تناوبی باشدت بالا (HIIT)، تقسیم شدند. تمرینات به مدت 10 هفته،5  بار در هفته انجام شد. پروتکل گروه استقامتی تداومی با شدت65 -60 درصد vo2max و گروه تناوبی با شدت بالا با شدت 90-85 درصد  vo2max اجرا شد. میزان بیانMIR-205  وژن VEGF به روش Real time-PCR اندازه­گیری شد. یافته‌ها: میزان بیان MIR-205 در گروه تناوبی باشدت بالا (000/0=p)، و در گروه استقامتی تداومی (001/0=p)، نسبت به گروه کنترل افزایش معناداری داشت. میزان بیان ژنVEGF  در گروه تناوبی باشدت بالا (003/0=p)، و در گروه استقامتی تداومی (005/0=p)، نسبت به گروه کنترل کاهش معناداری داشت. نتیجه‌گیری: با توجه به اثر افزایشی تمرین تناوبی با شدت بالا و استقامتی تداومی بر بیان MIR-205 و اثر کاهشی بر بیان ژن VEGF در موش‌های مبتلا به سرطان پستان، این دو نوع تمرین می‌تواند در روند کاهشی رشد تومور موثر باشد. همچنین با توجه به نتایج، تمرینات تناوبی با شدت بالا با توجه به مدت زمان کمتر، ممکن است  موثرتر است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Ten Weeks of High Intensity Interval Training and Continuous Endurance Training on The Expression of MIR-205 and VEGF Gene in Mice with Breast Cancer Has Been Compared

نویسندگان [English]

  • Ziba Rezaei 1
  • Saeid Shakerian 2
  • Masoud Nikbakht 3
1 Msc of Exercise Physiology, Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Associate Professor of Exercise Physiology, Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Associate Professor of Exercise Physiology, Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
چکیده [English]

Aim : Many genes are involved in the development of breast cancer. One is the VEGF (Vascular Endothelial Growth Factor) gene, one of the most important angiogenesis factors. On the other hand, one of the suppressive microRNAs is MIR-205, which is significantly less translatable in breast tumors compared to healthy matched tissue. Therefore, in this study, we compared the effect of 10 weeks of high intensity periodic training with continuous endurance training on MIR-205 and the VEGF gene in mice with breast cancer. Methods: The study was performed on 18 Balbci mice, with a mean body mass of 18-20g. The estrogen-dependent MC4-L2 carcinoma cell line was cultured, cultured, and then one million subcutaneous cancer cells were injected into the herb. One week after tumor formation, mice were randomly divided into three groups of 6: Control (C). ), Continuous endurance (E) and interval high intensity (H) were divided. Exercises were performed 5 times a week for 10 weeks. Each session of protocol E was 60-65% vo2max and group H was 90-85% vo2max. Expression of MIR-205 and VEGF was measured by Real-time PCR. Results: The expression level of MIR-205 was significantly in high intensity Interval group (p = 0.000), and in the Continuous endurance group (p = 0.001), than in the control group. The VEGF gene expression was significantly decreased in the in high intensity Interval group (p = 0.003) and in the Continuous endurance group (p = 0.005). Conclusions: Given the incremental effect of in high intensity Interval training and continuous endurance on MIR-205 expression and a decreasing effect on VEGF gene expression in mice with breast cancer, these two types of training may be effective in decreasing tumor growth. Also, according to the results, High intensity interval training due to less time, may be more effective.

کلیدواژه‌ها [English]

  • breast cancer
  • continuous endurance training
  • Hiit training
  • mir-205
  • VEGF
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