تاثیر هشت هفته تمرینات شنای تناوبی با شدت بالا بر بیان پروتئین های PGC-1α و IL-6و عملکرد حافظه در هیپوکمپ مغز رت های مبتلا به استئاتوهپاتیت غیرالکلی القا شده با رژیم پرچرب

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکدة علوم ورزشی و تندرستی، دانشگاه تهران، تهران-ایران

2 استاد فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکدة علوم ورزشی و تندرستی، دانشگاه تهران، تهران-ایران

3 دانشیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکدة علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز-ایران

10.22049/jahssp.2023.28611.1552

چکیده

هدف: استئاتوهپاتیت غیرالکلی (NASH) پیامد تجمع بیش از حد تری­گلیسرید در سلول­های کبد است که با کاهش PGC-1α و افزایش سایتوکاین­های التهابی همراه است. تحقیق حاضر با هدف بررسی تاثیر هشت هفته تمرین تناوبی­ شنا با شدت بالا بربیان پروتئین­های  PGC-1α و IL-6 و عملکرد حافظه در هیپوکمپ مغز در رت­های مبتلا به استئاتوهپاتیت غیرالکلی القا شده با رژیم پرچرب انجام شد. روش شناسی: تحقیق حاضر از نوع تجربی بود. 40 سر رت نر نژاد اسپراگوداولی به دو گروه سالم (20=n) و رژیم پرچرب (HFD) (20=n) تقسیم شدند. گروه  HFDبا هدف القا بیماری، به­مدت هشت هفته رژیم­غذایی پرچرب دریافت کردند. پس از اثبات بیماری، رت‌ها به چهار گروه کنترل-سالم(9=n)، تمرین-سالم(9=n)، کنترل-بیمار (9=n) و تمرین-بیمار(9=n) تقسیم­ شدند. گروه‌های تمرینی هشت هفته تمرینات شنای تناوبی با شدت بالا (سه جلسه­ در ­هفته، هرجلسه 30 دقیقه) انجام دادند. در پایان، پروتئین­های PGC-1α و IL-6 درون هیپوکمپ مغز به روش الایزا اندازه­گیری شد و عملکرد حافظه با آزمون رفتاری ماز آبی موریس ارزیابی شد. جهت آنالیز داده­ها از آزمون آنالیز واریانس یک­طرفه با آزمون تعقیبی بونفرونی استفاده شد (05/0>p) و همه روش­های آماری با استفاده از نرم­افزارversion 26   spss انجام شد. یافته‌ها: بالاترین و پایین­ترین مقدار پروتئین­ PGC-1αبه ترتیب در گروه­های تمرین-سالم و کنترل-بیمار مشاهده شد. هم­چنین در متغیرIL-6 بالاترین و پایین­ترین میزان به ترتیب در گروه­های کنترل-بیمار و تمرین-سالم مشاهده گردید. در متغیر عملکرد حافظه نیز بین­ گروه­ها تفاوت معنادار وجود داشت(05/0>P). نتیجه‌گیری: تمرینات شنای تناوبی با شدت بالا در NASH می تواند منجر­ به افزایش PGC-1α و کاهش IL-6 در هیپوکمپ مغز شود و عملکرد حافظه را بهبود بخشد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of eight weeks of high-intensity interval swimming training on the expression of PGC-1α and IL-6 proteins and memory function in brain hippocampus in rats with non-alcoholic steatohepatitis induced by high fat diet

نویسندگان [English]

  • maryam abbasi 1
  • mohammadreza kordi 2
  • Farhad Daryanoosh 3
1 Master Student of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran.
2 Professor of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran.
3 Associate Professor in Exercise Physiology, Faculty of Educational Sciences and Psychology, Department of Sports Sciences, Shiraz University, Shiraz, Iran
چکیده [English]

Aim: Nonalcoholic steatohepatitis (NASH) is the result of excessive accumulation of triglycerides in liver cells, which is associated with a decrease in PGC-1α and an increase in inflammatory cytokines. The present study was conducted with the aim of investigating the effect of eight weeks of high-intensity interval swimming training on the expression of PGC-1α and IL-6 proteins and memory function in the brain hippocampus in rats with non-alcoholic steatohepatitis induced by a high-fat diet. Methods: The present research was experimental. 40 male Sprague-Dawley rats were divided into two groups: healthy (n=20) and high-fat diet (HFD) (n=20). The HFD group received a high-fat diet for eight weeks with the aim of inducing the disease. After proving the disease, the rats were divided into four groups: control-healthy (n=9), training-healthy (n=9), control-disease (n=9), and training-disease (n=9). The training groups performed eight weeks of high-intensity interval swimming exercises (three sessions per week, each session lasting 30 minutes). In the end, PGC-1α and IL-6 proteins in the hippocampus of the brain were measured by the ELISA method, and memory performance was evaluated by the Morris water Maze behavioral test. One-way analysis of variance with the Bonferroni post hoc test was used for data analysis (p<0.05) and all statistical methods were performed using spss version 26 software. Results: The highest and lowest amount of PGC-1α protein was observed in training-healthy and control-disease groups, respectively. Also, in the IL-6 variable, the highest and lowest levels were observed in the control-disease and training-healthy groups, respectively. There was also a significant difference between the groups in the variable of memory performance (P<0.05). Conclusion: high-intensity interval swimming training in NASH can lead Increase in PGC-1α and decrease IL-6 in the brain hippocampus and improve memory function.

کلیدواژه‌ها [English]

  • Nonalcoholic steatohepatitis
  • high-intensity interval swimming
  • hippocampus
  • memory function

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