تاثیر 12 هفته تمرین تناوبی خیلی شدید بر بیان پروتئین‌ PGC-1α, SIRT1 ERRα, در رت‌های سالمند

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی دانشگاه تهران، دانشگاه تهران، ایران

2 استاد،گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی دانشگاه تهران، دانشگاه تهران، ایران

3 دانشیار ، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی دانشگاه تهران، دانشگاه تهران، ایران

4 دانشیار مرکز تحقیقات درمان و پیشگیری از چاقی، پژوهشکده علوم غدد درون ریز و متابولیسم، دانشگاه علوم پزشکی و خدمات بهداشتی-درمانی شهید بهشتی، دانشگاه شهید بهشتی تهران، ایران

10.22049/jassp.2019.26555.1223

چکیده

تمرین ورزشی به بهبود آسیب عملکرد پروتئین‌ها و آنزیم‌های میتوکندری در سالمندی منجر می‌شود. هدف پژوهش حاضر تاثیر 12 هفته تمرین تناوبی خیلی شدید (HIIT) بر بیان پروتئین‌های میتوکندریایی PGC-1α, SIRT1 ERRα, در رت‌های سالمند بود. در این مطالعه تعداد 30 رت نژاد ویستار سالمند به صورت تصادفی به دو گروه HIIT و گروه کنترل (CTR) تقسیم شدند. تمرین ورزشی در گروه HIIT در هفته اول 16 دقیقه (2 دقیقه با شدت 85 تا 90 درصد VO2max و 2 دقیقه با شدت 45 تا 50 درصد VO2max) آغاز شد و به تدریج تا هفته دوازدهم به 28 دقیقه رسید. مقادیر بیان پروتئین-های PGC-1α, SIRT1 و ERRα عضله دو قلو به روش وسترن بلات سنجیده شد. از روش آماری تی مستقل برای تجزیه تحلیل داده‌ها استفاده شد. وزن بدن و وزن عضله دو قلو بعد از 12 هفته HIIT تفاوت معنا داری نداشت. بیان پروتئین PGC-1α و ERRα در گروه HIIT در مقایسه با گروه کنترل افزایش معناداری داشت (P<0.001). بیان پروتئین SIRT1 در گروه HIIT در مقایسه با گروه کنترل افزایش معناداری داشت (P<0.01). به نظر می رسد HIIT به وسیله شدت بالا و ماهیت تمرین تناوبی باعث افزایش بیان پروتئین‌های PGC-1α, Sirt1 ERRα, عضله دوقلو رت‌های سالمند شده است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of 12-weeks high-intensity interval training on SIRT1, PGC-1α and ERRα protein expression in aged rats

نویسندگان [English]

  • Ali Bakhtiyari 1
  • Abasali Gaeni 2
  • Siros Chobineh 3
  • Mohammad Reza Kordi 3
  • Mehdi Hedayati 4
1 PhD Student, Department of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, University of Tehran, Iran.
2 Professor, Department of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, University of Tehran, Iran.
3 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, University of Tehran, Iran
4 Associate Professor, Shaheed Beheshti University of Medical Sciences Cellular and Molecular Biology Research center, Tehran, Iran
چکیده [English]

Exercise training improved impaired mitochondrial protein and enzymatic functional induced aging. The purpose of this study was to investigate the effects of 12-weeks of high-intensity interval training on mitochondrial proteins of SIRT1, PGC-1α and ERRα in gastrocnemius muscle in aged rats. In this study 30 aged Wistar rats were randomly divided into HIIT and control group. Exercise training was performed 3 days per week for 8-weeks. Exercise training in the HIIT group was started in the first week by 16 minutes (four intervals (T=2min) at 85-90% VO2max with 4 recovery periods (T=2min) at 45-50% VO2max), which reached 28 minutes in the 12th week. Gastrocnemius was removed 48 h after the final training session. The expression levels of SIRT1, PGC-1α and ERRα proteins in gastrocnemius were assessed by Western blot method. Data analysis was done with independent samples t test. There were no significant differences in body weight and gastrocnemius muscle weight after the intervention. The protein expression of PGC-1α and ERRα were significantly increased in the HIIT compared to control group (P<0.001). Moreover, SIRT1 expression was significantly elevated in the HIIT compared to control group (P <0.01). It seems that HIIT due to its high intensity and interval nature leads to an increased expression of SIRT1, PGC-1α and ERRα proteins in the gastrocnemius muscle of aging rat models.

کلیدواژه‌ها [English]

  • : Aging
  • High-intensity interval training
  • SIRT1
  • PGC-1α

 

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