پاسخ ضد التهابی یک جلسه بازتوانی هوازی به‌همراه تحریک ‌الکتریکی در رت‌های القاء شده به سکته قلبی

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، واحد اراک، دانشگاه آزاد اسلامی، اراک، ایران.

10.22049/jahssp.2023.28543.1550

چکیده

هدف: گلیکوپروتئین‌های پنتراکسین-3 و E-سلکتین هنگام التهاب، توسط سلول‌های اندوتلیوم، ماکروفاژها و سلول‌های میلوئیدی سنتز شده و نقش اساسی در بیماران سکته  قلبی دارد. با توجه به اثرات مثبت بازتوانی هوازی بر بیماران قلبی تحقیق حاضر به‌بررسی پاسخ ضد التهابی یک جلسه بازتوانی هوازی به‌همراه تحریک ‌الکتریکی در رت‌های القاء شده به سکته قلبی پرداخته است. روش‌ شناسی: در این مطالعه تجربی کنترل شده با گروه شاهد 50 سر رت با وزن (30 ±200 گرم) به‌طور تصادفی به 5 گروه برابر سالم، سکته قلبی، سکته قلبی-بازتوانی هوازی، سکته قلبی-تحریک‌الکتریکی و سکته-بازتوانی‌هوازی-تحریک‌الکتریکی تقسیم شدند. سپس سکته قلبی با تزریق زیرجلدی ایزوپروترونول القاء گردید. گروه‌های مداخله برای یک جلسه تحت بازتوانی هوازی (تردمیل با سرعت 20 متر/دقیقه برای 1 ساعت) و تحریک‌الکتریکی (با فوت شوک برای 5/0 میلی‌آمپر و 20 دقیقه) قرار گرفتند. سپس بلافاصله بعد از مداخله، سطوح سرمی متغیرها به روش الایزا بررسی شد. آنالیز داده‌ها با آزمون آنووآ یک­طرفه و معنی‌داری 05/0>P انجام شد. یافته‌ها: نتایج نشان داد که القاء سکته قلبی منجر به افزایش معنی‌دار سطوح سرمی پنتراکسین-3 و E-سلکتین مطالعه حاضر شد  (0001/0P=). انجام بازتوانی و تلفیقی از بازتوانی و تحریک‌الکتریکی تاثیر معنی‌داری بر مقادیر پنتراکسین-3  نداشت  (943/0P=)، اما غلظت E-سلکتین در کلیه گروه‌ها نسبت به سکته قلبی کاهش داشت که این تغییر در گروه بازتوانی و تحریک‌الکتریکی معنی‌دار بود (044/0P=). نتیجه‌گیری: به‌نظر می‌رسد بازتوانی هوازی، تحریک‌الکتریکی و تلفیقی از آن‌ها با کاهش مقادیر E-سلکتین می‌تواند منجر به کاهش التهاب بیماران با سکته قلبی شود و در بهبود حال آن‌ها موثر باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Anti-inflammatory response of an aerobic rehabilitation session with electrical stimulation in rats induced with myocardial infarction

نویسندگان [English]

  • Mohammad Malekipooya
  • Mojtaba Khansooz
Assistant Professor, Department of Exercise Physiology, Arak Branch, Islamic Azad University, Arak, Iran
چکیده [English]

Aim: Pentraxin-3 and E-selectin glycoproteins are synthesized by endothelium cells, macrophages and myeloid cells during inflammation and play an essential role in heart attack patients. Considering the positive effects of aerobic rehabilitation on heart patients, the present research has investigated the anti-inflammatory response of an aerobic rehabilitation session accompanied by electrical stimulation in rats induced to myocardial infarction. Methods: In this controlled experimental study with a control group, 50 rats (200±30 gram) were randomly divided into 5 groups: healthy, infarcted, infarcted-aerobic rehabilitation, infarcted-electrical stimulation, and infarcted-aerobic rehabilitation-electrical stimulation. Then infarction was induced by subcutaneous injection of Isoproterenol. The intervention groups underwent aerobic rehabilitation (treadmill at a speed of 20 m/min for 1 hour) and electrical stimulation (with foot shock for 0.5 mA and 20 minutes) for one session. Then, immediately after the intervention, the serum levels of the variables were checked by ELISA method. Data analysis was done with one-way ANOVA test and P<0.05 significance. Results: The results showed that infarct induction led to a significant increase in pentraxin-3 and E-Selectin serum levels in the present study (P=0.0001). Performing rehabilitation and a combination of rehabilitation and electrical stimulation had no significant effect on pentraxin-3 values (P=0.943). But E-selectin concentration was decreased in all groups compared to infarction, and this change was significant in rehabilitation and electrical stimulation group (P=0.044). Conclusion: It seems that aerobic rehabilitation, electrical stimulation, and a combination of them by reducing E-selectin levels can be effective in improving the condition of myocardial infarction samples by reducing inflammation.

کلیدواژه‌ها [English]

  • Pentraxin-3
  • E-selectin
  • Aerobic Rehabilitation
  • Electrical Stimulation

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 10، شماره 2
مهر 1402
صفحه 139-150

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  • تاریخ دریافت: 18 فروردین 1402
  • تاریخ بازنگری: 29 تیر 1402
  • تاریخ پذیرش: 29 تیر 1402

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