تأثیر 8 هفته تمرین مقاومتی بر عملکرد عضلانی و برخی پروتئین‌های مرتبط با سارکوپنی در عضله نعلی رت‌های نر سالمند چاق

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزش، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

2 دانشیار گروه فیزیولوژی ورزش، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.

3 استادیار گروه فیزیولوژی ورزش، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.

10.22049/jahssp.2023.28239.1537

چکیده

هدف: تمرین مقاومتی یکی از مؤثرترین روش‌ها برای کاهش بافت چربی و جلوگیری از کاهش توده و قدرت عضلانی (سارکوپنی) است. هدف از پژوهش حاضر بررسی تأثیر 8 هفته تمرین مقاومتی بر مقادیر پروتئین‌های Atrogin-1 و  Murf1  FOXO3,و همچنین قدرت عضلانی رت‌های نر سالمند چاق بود. روش‌شناسی: از تعداد 30 سر رت نر نژاد ویستار (سن: 15 ماه و وزن: 320 تا 350 گرم)، 10 سر به عنوان گروه کنترل جدا و مابقی به مدت 12 هفته تحت رژیم غذایی پرچرب چاق شدند. سپس رت ­های چاق (با شاخص لی بالاتر از 310) به دو گروه کنترل چاق و تمرین مقاومتی تقسیم شدند. گروه تمرین مقاومتی برنامه تمرینی (بالا رفتن از نردبان) را به مدت 8 هفته و هر هفته سه جلسه انجام دادند (3 نوبت با 8 تکرار و با 25%، 50%، 75% و 100% وزن بدن). مقادیر پروتئین‌های Atrogin-1 و  Murf1 FOXO3, به روش الایزا در عضله نعلی اندازه‌گیری شد. قدرت عضلانی نیز با آزمون قدرت گرفتن دو اندام جلویی اندازه‌گیری شد. مقدار پروتئین­ها با استفاده از روش تحلیل واریانس یک‌راهه و آزمون‌ تعقیبی توکی و قدرت عضلات با آزمون کوواریانس و آزمون تعقیبی بانفرونی تحلیل شدند. یافته‌ها: در مقایسه با گروه کنترل، چاقی باعث افزایش پروتئین‌های Atrogin-1 و  Murf1 FOXO3, ( 000/0p=) و کاهش قدرت عضلانی (000/0p=) در گروه کنترل چاق شد. از طرف دیگر، تمرین مقاومتی مقادیر ( 012/0p=) Atrogin-1 و ( 005/0p=) Murf1  , (003/0p=) FOXO3 را در گروه تمرین به نسبت گروه کنترل چاق کاهش و قدرت گرفتن دو اندام جلویی را افزایش داد. (000/0p=). نتیجه‌گیری: اگرچه چاقی باعث افزایش نشانگرهای آتروفی عضلانی شد، نتایج این پژوهش نشان داد که تمرین مقاومتی با کاهش نشانگرهای مرتبط با آتروفی عضلانی، نقش مهمی در حفظ توده و افزایش قدرت عضلانی در رت­های سالمند چاق دارد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of 8 weeks of resistance training on muscle function and some proteins related to sarcopenia in soleus muscle of obese aged male rats

نویسندگان [English]

  • Esmaeil Azimian 1
  • Ali Akbarnejad Gharehloo 2
  • Parisa Pournemati 3
1 PhD student of exercise physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
2 Associate Professor of Exercise Physiology Department, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
3 Assistant Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran , Tehran, Iran.
چکیده [English]

Aim:   Resistance training is one of the most effective methods to reduce fat tissue and   prevention of reducing muscle mass and strength loss (sarcopenia). The aim of this study was to investigate the effect of 8 weeks of resistance training on protein amounts of FOXO3, Murf1 and Atrogin-1 and also muscle strength of obese aged male rats. Method: Out of 30 Wistar male rats (age: 15 months and weight: 320 to 350 grams), 10 were separated as control group and the rest became obese under a high-fat diet for 12 weeks.  Then the obese rats (with Lee index higher than 310) were divided into two groups: obese control and resistance training. The resistance training group performed training program (ladder-climbing) for 8 weeks, 3 sessions per week (3 sets of 8 reps with %25, %50, %75 and %100 of body weight). The amounts of FOXO3, Murf1 and Arogin-1 proteins were measured by ELISA method in soleus muscle. muscle strength was also measured by forelimb grip strength test. The levels of protein were analyzed using ANOVA and Tukey and muscle strength were analyzed with ANCOVA and Bonferroni post hoc test. Results: Compared to the control group, obesity increased FOXO3, Murf1 and Arogin-1 proteins (p=0.000) and decreased muscle strength (p=0.000) in the obese control group. on the other hand, resistance training decreased the values of FOXO3 (p=0.003), Murf1 (p=0.005) and Arogin-1 (p=0.012) and increased forelimb grip strength (p=0.000) in the training group compared to the obese control group. Conclusion: Although obesity increased muscle atrophy markers, the results of this research showed that resistance training plays an important role in maintaining muscle mass and increasing muscle strength in obese aged rats by reducing muscle atrophy markers.

کلیدواژه‌ها [English]

  • Resistance Training
  • Obesity
  • Aging
  • Sarcopenia
  • Wistar Rats

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 10، شماره 2
مهر 1402
صفحه 13-26

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  • تاریخ دریافت: 25 دی 1401
  • تاریخ بازنگری: 09 فروردین 1402
  • تاریخ پذیرش: 09 فروردین 1402

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