تاثیر تمرینات تناوبی شدید همراه با مصرف عصاره خرفه برسطح سیرتونین1 و مقاومت به انسولین رت های مبتلا به کبد چرب غیر الکلی

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران

10.22049/jahssp.2022.28057.1506

چکیده

هدف: سیرتوئین-1 (Sirt1) با مقاومت به انسولین در ارتباط است و مقاومت به انسولین می­تواند زمینه را برای ابتلا و پیشرفت بیماری کبد چرب غیر الکلی (NAFLD) فراهم کند. بنابراین هدف از این تحقیق بررسی تاثیر تمرینات تناوبی شدید (HIIT) و مصرف عصاره خرفه بر سطح  Sirt1کبدی و مقاومت به انسولین رت­های مبتلا به  NAFLDبود. روش شناسی: تعداد 20سر رت نر بالغ ویستار با سن شش هفته انتخاب و پس از القای کبد چرب غیر الکلی، به صورت تصادفی در 4 گروه کنترل کبد چرب ،HIIT ، عصاره خرفه و HIIT+عصاره خرفه قرار داده شدند. عصاره خرفه با دوز 400 میلی گرم به ازای هر کیلوگرم با توجه به وزن رت­ها به دو گروه مربوطه خورانده شد. تمرینHIIT نیز به مدت 8 هفته ، 5 جلسه در هفته و هر جلسه 7 تکرار یک دقیقه ای باشدت ۹۰ درصد سرعت بیشینه انجام شد که تناوب­های استراحت فعال نیز شامل 2 دقیقه دویدن با شدت ۲۰ درصد بیشینه بود. سطح  Sirt1کبدی و مقاومت به انسولین بعد از 8 هفته HIIT و مصرف عصاره خرفه اندازه گیری شد. برای تجزیه و تحلیل داده­ها از آنالیز واریانس یک طرفه و آزمون تعقیبی توکی در سطح معنی داری 05/0>p استفاده شد. یافته ­ها: میزان مقاومت به انسولین در گروه کنترل نسبت به گروه  HIIT(012/0=p)، عصاره خرفه (037/0=p) و HIIT+عصاره (014/0=p) به­طور معنی­داری بالاتر بود. سطح Sirt1 کبدی در گروه کنترل به­طور معنی­داری نسبت به گروه HIIT (035/0=p)، عصاره خرفه (01/0=p) و HIIT+عصاره (001/0=p) به­طور معنی­داری پایین­تر بود. سطح  Sirt1کبدی در گروه مصرف عصاره خرفه با گروه­های HIIT و HIIT+عصاره تفاوتی مشاهده نشد (مقادیر p به ترتیب 914/0 و 106/0) اما سطح Sirt1 کبدی در گروه HIIT نسبت به گروه HIIT+عصاره به­طور معنی­داری پایین­تر بود (031/0=p). نتیجه گیری: به نظر می­رسد تمرین ورزشی و مصرف عصاره خرفه از طریق افزایش سطح Sirt1 در بیماران مبتلا بهNAFLD بهبود مقاومت به انسولین در این بیماران را در پی داشته باشد و احتمالاً نقش مهمی را در کنترل پیشرفت این بیماری بر عهده داشته باشند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of High-Intensity Interval Training and Portulaca Oleracea extract on Sirtuin1 Level and Insulin Resistance in Rats with Non-Alcoholic Fatty Liver Disease

نویسندگان [English]

  • mohammad Ranaei
  • Ali Yaghoubi
  • Sadegh Cheragh-Birjandi
Department of Physical Education and Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
چکیده [English]

Aim: Sirtuin1 (Sirt1) levels is associated with insulin resistance and insulin resistance can provide conditions for the development of non-alcoholic fatty liver disease (NAFLD). Thus the aim of this study was to investigate the effect of eight weeks of high intensity interval training (HIIT) and PortulacaOleracea extract on Sir1 level and insulin resistance in rats with NAFLD. Methods: Twenty male Wistar rats aged six weeks were selected and after induction of non-alcoholic fatty liver,were randomly divided into four groups: control, HIIT, PortulacaOleracea extract, and HIIT+PortulacaOleracea extract. To induce NAFLD, the rats were fed a high-fat diet for 12 weeks. PortulacaOleracea supplement at 400 mg/kg was given to the experimental groups. HIIT was performed for 8 weeks, 5 sessions per week with 7 repetitions of 1 minute at 90% maximum speed accompanied by active rest intervals including 2 minutes of running at 20% maximum speed. Hepatic Sir1 level and insulin resistance were measured after 8 weeks of HIIT and consumption of purslane extract. To analyze the data, one-way analysis of variance and Tukey's post hoc test were used at the significance level of p<0.05. Results: Insulin resistance was significantly higher in control group than HIIT (P=0.012), PortulacaOleracea extract (P=0.037), and HIIT+PortulacaOleracea extract (P=0.014) groups.Sirt1 levels in control group was significantly lower than HIIT (p=0.035), PortulacaOleracea extract (P=0.01), and HIIT+PortulacaOleracea extract (P=0.001) groups. The level of liver Sirt1 in the PortulacaOleracea extract group was not different from the HIIT and HIIT+ PortulacaOleracea extract groups (p values 0.914 and 0.106, respectively), but the liver Sirt1 level in the HIIT group was significantly lower than the HIIT+extract group (p=0.031).Conclusion: It seems that HIIT and PortulacaOleracea extract could improve insulin resistance in NAFLD by increasing Sirt1 levels and can play an important role in controlling the progress of this disease.

کلیدواژه‌ها [English]

  • High-intensity interval training
  • Portulaca Oleracea extract
  • Sirtuin 1
  • insulin resistance
  • non-alcoholic fatty liver disease

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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