اثرات هشت هفته تمرین هوازی و رزوراترول بر بیان ژن‌های دخیل در ترموژنز و پروفایل لیپیدی در موش‌های نر نژاد C57BL/6

نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I

نویسنده

گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه کاشان، کاشان، ایران

10.22049/jahssp.2022.27762.1459

چکیده

هدف: هدف مطالعه حاضر بررسی اثر ترکیبی رزوراترول و تمرین هوازی بر بیان ژن­های درگیر در ترموژنز و پروفایل لیپیدی در بافت چربی زیرپوستی موش­های نژاد C57BL/6 بود. روش شناسی: 32 موش نر با میانگین وزنی 4±16 گرم به طور تصادفی به چهار گروه (تعداد = 8) تقسیم شدند. 1. رزوراترول (Res)، 2. کنترل (Control)، 3. تمرین هوازی (Exe)، 4. تمرین هوازی+رزوراترول(Res+Exe). موش­ها رزوراترول را به مدت هشت هفته، پنج مرتبه/هفته، از طریق گاواژ دریافت کردند. موش­های تمرین کرده به مدت هشت هفته، پنج جلسه/هفته با میانگین شدت 50-65 درصد حداکثر اکسیژن مصرفی تحت تمرین بدنی بر روی نوارگردان قرار گرفتند. پس از اتمام پروتکل پژوهش و 24 ساعت پس از آخرین جلسه تمرین، بافت چربی زیرپوستی استخراج و بیان ژن­ها (PGC1a، FNDC5، UCP1، PRDM16 و (SIRT1 در بافت چربی با استفاده از تکنیک Real-time PCR سنجش شد. همچنین میزان پروفایل چربی (تری گلیسیرید، کلسترول تام، HDL و LDL) ارزیابی شد. یافته ­ها: نتایج نشان داد که سطوح FNDC5، SIRT1 و UCP1 در بافت چربی موش­های گروه Res بالاتر از گروه کنترل بود. همچنین بیان PGC1α، FNDC5، UCP1، PRDM16 و SIRT1 در بافت چربی موش­های Exe بالاتر از گروه کنترل بود. نشانگرهای ترموژنز و ژن­های دخیل در قهوه ای شدن بافت چربی در گروه Res+Exe نسبت به گروه Res و Exe به طور معناداری بالاتر بود. میزان HDL در گروه Res+Exe نسبت به گروه کنترل بالاتر و سطوح LDL، تری گلیسرید و کلسترول تام در گروه Res+Exe نسبت به گروه کنترل پایین­تر بود. نتیجه گیری: تمرین هوازی و رزوراترول اثر هم­افزایی قابل توجهی بر افزایش ترموژنز و افزایش بیان UCP1، PRDM16، PGC1α،SIRT1  و FNDC5 نسبت به گروه تمرین و رزوراترول به تنهایی دارد. افزایش بیان FNDC5 در بافت چربی زیرجلدی که به دنبال مداخله تمرین هوازی و رزوراترول ایجاد می­گردد، توسط SIRT1 تعدیل می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of eight weeks of aerobic exercise and resveratrol on expression of genes involved in thermogenesis and lipid profile in male C57BL/6 mice

نویسنده [English]

  • Fatemeh Kazeminasab
Department of Physical Education and Sport Sciences, Faculty of Human Sciences, University of Kashan, Kashan, Iran
چکیده [English]

Aim: The aim of this study was to evaluate the combined effect of resveratrol and aerobic exercise on the expression of genes involved in thermogenesis and lipid profile in the subcutaneous adipose tissue of C57BL/6 mice. Methods: Thirty-two male mice with a mean weight of 16±4 g were randomly divided into four groups (n=8).  1. Resveratrol (Res), 2. Control, 3. Aerobic exercise (Exe), 4. Resveratrol+Aerobic exercise (Res+Exe). The mice received resveratrol by gavage five times/week for eight weeks. The exercised mice trained on a treadmill for eight weeks, five sessions/week with an average intensity of 65-50% of maximum oxygen consumption (Vo2max). Adipose tissue was extracted after completing the research protocol and 24 hours after the last training session, subcutaneous adipose tissue was extracted and the expression of genes (PGC1a, FNDC5, UCP1, PRDM16, and SIRT1) in adipose tissue was measured using Real-time PCR. Results:  The results showed that the levels of FNDC5, SIRT1, and UCP1 in the adipose tissue of Res mice were higher than the control group.  Also, the expression of PGC1α, FNDC5, UCP1, PRDM16, and SIRT1 in the adipose tissue of Exe mice was higher than the control group. The markers of thermogenesis and genes involved in adipose tissue browning were significantly in the Res+Exe group higher than the Res and Exe groups.  The levels of HDL in the Res+Exe group were higher than the control group and the levels of LDL, triglyceride and total cholesterol were lower in the Res+Exe group than the control group. Conclusions:  Aerobic exercise and resveratrol have a significant synergistic effect on increased thermogenesis and increased expression of UCP1, PRDM16, PGC1α, SIRT1, and FNDC5 compared to Exe and Res group alone.  Increased expression of FNDC5 in subcutaneous adipose tissue following modification of aerobic exercise and resveratrol is modulated by SIRT1.

کلیدواژه‌ها [English]

  • Resveratrol
  • aerobic exercise
  • Thermogenesis
  • Sirtuin 1 (SIRT1)
  • Fibronectin type III domain-containing protein 5 (FNDC5)
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دوره 9، شماره 1
فروردین 1401
صفحه 164-175
  • تاریخ دریافت: 21 فروردین 1401
  • تاریخ بازنگری: 21 خرداد 1401
  • تاریخ پذیرش: 23 خرداد 1401
  • تاریخ اولین انتشار: 23 خرداد 1401