تاثیر تمرین هوازی بر مقدار ادم پنجه و سطوح IL-6 ، TNF-α و CC16 سرم موش‌های نر تحت مواجهه با آرسنیک

نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I

نویسندگان

گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، دانشگاه آزاد اسلامی واحد تبریز، تبریز، ایران.

10.22049/jahssp.2022.27769.1460

چکیده

هدف: قرار گرفتن در معرض آرسنیک از طریق آب آشامیدنی یک مشکل بهداشت عمومی گسترده است که با افزایش خطر پیامدهای متعددی از جمله التهاب همراه است. هدف از این تحقیق، تعیین تاثیر تمرین هوازی بر مقدار ادم پنجه و سطوح IL-6 ، TNF-α و CC16 سرم موش‌های نر تحت مواجهه با آرسنیک بود. روش شناسی: در این مطالعه تجربی، تعداد 32 سر موش صحرائی نر به چهار گروه شامل کنترل، آرسنیک، تمرین، و آرسنیک+تمرین تقسیم شدند. آرسنیک به مدت 8 هفته روزانه از طریق آب آشامیدنی با دوز پنج میلی‌گرم بر هر کیلوگرم وزن بدن به صورت گاواژ خورانده شد. تمرین به مدت هشت هفته با تکرار پنج روز در هفته، با سرعت 10 تا 25 متر در دقیقه، شیب 5 درصد و روزانه به مدت 30-10 دقیقه بر روی تردمیل ویژة جوندگان آزمایشگاهی انجام شد. از روش‌های الایزا و حجم سنج پلتیسمومتر به ترتیب برای اندازه گیری سطوح IL-6 ، TNF-α و CC16 سرم و مقدار ادم پنجه پای موش استفاده شد. داده‌ها با روش تحلیل واریانس یک راهه و آزمون تعقیبی توکی در سطح معنی‌داری 05/0>p تحلیل شدند. یافته‌ها: مواجهه با آرسنیک، سبب افزایش سطوح IL-6 ، TNF-α و کاهش CC16 سرم موش‌های نر شد (001/0=p برای هر سه متغیر)، اما بر ادم پنجه پای موش تاثیر معنی‌داری نداشت (8/0=p). مداخله تمرین در مقایسه با گروه کنترل، آرسنیک و آرسنیک+تمرین، افزایش سطوح IL-6  (به ترتیب 001/0=p، 02/0=p، 001/0=p)، TNF-α (به ترتیب 001/0=p، 02/0=p، 03/0=p) و کاهش CC16 سرمی (به ترتیب 03/0=p، 001/0=p، 008/0=p) ناشی از آرسنیک را افزایش داد. با اینحال، تمرین بر ادم پنجه پای موش تاثیر معنی‌داری نداشت (8/0=p).  نتیجه‌گیری: در حالی که مواجهه با آرسنیک می‌تواند به افزایش سطوح IL-6، TNF-α و کاهش CC16 سرمی موش‌ها منجر شود، به نظر می رسد که تمرین هوازی این پاسخ‌های التهابی را برعکس می‌کند. با این حال، به دلیل برخی محدودیت‌ها و کمبود تحقیقات انسانی، به بررسی‌های بیشتر نیاز است. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of aerobic training on the amount of paw edema and IL-6, TNF-α and CC16 levels in rats exposed to arsenic

نویسندگان [English]

  • Amir Dadashzadeh
  • Roghayeh Poozesh Jadidi
Department of Sport Physiology, Faculty of Humanities, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
چکیده [English]

Aim: Exposure to arsenic through drinking water is a widespread public health problem that is associated with an increased risk of several consequences, including inflammation. The aim of the present study was to evaluate effect of aerobic training on the amount of paw edema and IL-6, TNF-α and CC16 levels in rats exposed to arsenic. Methods: In this experimental study, 32 male rats were randomly divided into four groups: control, arsenic, training and arsenic+training. Arsenic  was consumed  five mg/kg/day orally (gavaged) for eight weeks. Aerobic training was conducted  for eight weeks (five sessions/week, each session for 10 to 30 min with a treadmill speed of 10 to 25 m/min, at slope of 5%. ELISA and plethysmometer methods were used to measure serum IL-6, TNF-α and CC16 levels and the amount of rat paw edema, respectively. Data were analyzed by one-way analysis of variance and Tukey post hoc tests at the p<0.05 level. Results: Arsenic exposure elevated IL-6, TNF-α and CC16 levels (For all three variables p=0.001) in rats exposed to arsenic, but had no significant effect on paw edema (p=0.8). Training intervention decreased arsenic-induced serum levels of IL-6 (respectively p=0.001, p=0.02,  p=0.001), TNF-α (respectively p=0.001, p=0.02,  p=0.03) and CC16 (respectively p=0.03, p=0.001,  p=0.008) compared to the control, arsenic and arsenic + exercise groups  (p=0.8). However, training had no significant effect on rat paw edema (p <0.05). Conclusions: While, arsenic exposure leads to an increased serum IL-6, TNF-α and CC16 levels in rats exposed to arsenic, aerobic exercise seems to can reverse this inflammatory response. However, more researches remains to be done because of the study limitations and lack of similar evidence in human population.

کلیدواژه‌ها [English]

  • Arsenic
  • Inflammation
  • Edema
  • CC16
  • aerobic training
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دوره 9، شماره 1
فروردین 1401
صفحه 136-150
  • تاریخ دریافت: 23 فروردین 1401
  • تاریخ بازنگری: 20 اردیبهشت 1401
  • تاریخ پذیرش: 06 خرداد 1401
  • تاریخ اولین انتشار: 06 خرداد 1401