اثر هشت هفته تمرین تناوبی شدید و مصرف کافئین بر بیان پروتئین p38α و HSP70 در کبد موش های صحرایی دیابتی شده با استرپتوزوتوسین

نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزشی، گروه تربیت‌بدنی و علوم ورزشی، دانشکاه علامه قزوینی ، قزوین، ایران

2 دانشیار گروه فیزیولوژی ورزشی، گروه تربیت‌بدنی و علوم ورزشی، دانشکده علوم اجتماعی، دانشگاه بین المللی امام خمینی(ره) ، قزوین، ایران

3 استادیار گروه فیزیولوژی ورزشی، گروه تربیت‌بدنی و علوم ورزشی، دانشکده علوم اجتماعی، دانشگاه بین المللی امام خمینی(ره) ، قزوین، ایران

4 گروه تربیتب بدنی و علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، اردبیل، ایران

10.22049/jahssp.2022.27662.1445

چکیده

هدف: پروتئین‌هایی نظیر  p38αو  HSP70  از اهداف بالقوه درمان اختلالات دیابت نوع دو به ­شمار می­روند .هدف از این پژوهش بررسی هشت هفته تمرین تناوبی شدید و مصرف کافئین بر بیان پروتئین p38α و HSP70 در کبد موش‌های صحرایی دیابتی بود. روش­شناسی: در مطالعه‌ ‌تجربی حاضر، 50 سر موش صحرایی نر سفید نژاد ویستار با دامنۀ سنی 3-2 ماه به ‌طور تصادفی در 5 گروه 10 سری شامل: کنترل سالم(C)، کنترل دیابتی (D)، دیابتی تمرین کرده (D+T)، دیابتی دریافت کننده کافئین (D+CA) و دیابتی تمرین- کافئین (D+T+CA) تقسیم شدند. برنامه تمرین شامل 8 هفته و هفته‌ای 5 جلسه (6 تا 12 وهله 2 دقیقه‌ای با شدت 90- 85% سرعت بیشینه) بود و هر هفته پنج روز  70 mg/kg کافئین محلول با سالین تزریق شد. بـرای بررسـی پروتئین‌های p38α و HSP70 از روش وسترن بلات استفاده شد. داده‌ها با استفاده از آنالیز واریانس دوطرفه و در صورت مشاهده تفاوت معنادار آماری از آزمون توکی جهت تعیین اختلاف بین گروهی استفاده شـد  .یافته: سطح پروتئین HSP70 در موش‌های سالم و دیابتی با هم اختلاف معنادار داشت. تمرین تناوبی شدید و مصرف کافئین موجب افزایش معنادار  پـروتئین   HSP70نسبت به گروه  مکمل+ دیابتی شـد (001/0P=). گروه تمرین+ مکمل اثرگذاری بیشتری در افزایش  p38αنسبت به سایر گروه‌های دیابتی داشت، اما این تفاوت معنادار نبود (96/0P=). نتیجه‌گیری: تمرینات تناوبی شدید و مصرف کافئین با افزایش بیان HSP70 کبد می‌تواند نقشی مؤثری در مقابله با آسیب دیابت داشته باشد. با این حال، تمرینات تناوبی شدید و مصرف کافئین تاثیری بر بیان p38α  در کبد موش‌های صحرایی دیابتی ندارد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Eight-Week High Intensity Interval Training (HIIT) and Caffeine Intake On The p38α and hsp70 Protein expression in liver in Diabetic rats induced streptozotocin

نویسندگان [English]

  • Marya Rahmani 1
  • Abbas Sadeghi 2
  • Hassan Pourrazi 3
  • Seyed Hamed Ghiyami 4
1 MSc, , Department of Physical Education and Sports Science, Allameh Qazvini University, Qazvin, Iran
2 Associate Professor, Department of Physical Education and Sports Science, Faculty of Social Sciences, Imam Khomeini International University, Qazvin, Iran
3 Assistant Professor, Department of Physical Education and Sports Science, Faculty of Social Sciences, Imam Khomeini International University, Qazvin, Iran.
4 PhD student of Exercise Physiology, Department of Physical Education and Sports Science, Faculty of Educational Sciences and Psychology, Mohaghegh Ardabili University, Ardabil, Iran.
چکیده [English]

Aim:  Proteins such as p38α and HSP70 are considered as potential factors for type 2 diabetes disorders treatment. The aim of this study was to evaluate eight weeks of High-intensity Interval Training and caffeine consumption on p38α and HSP70 protein expression in the liver of diabetic rats. Method: 50 male Wistar rats with an age range of 2-3 months were randomly divided into 5 groups of 10 rats in each group: Healthy control (C), Diabetic control (D), Diabetic with Training (D+T), Diabetic with Caffeine treatment (D+CA), and Diabetic with Training and Caffeine treatment (D+ T + CA). The training program consisted of 8 sessions and 5 sessions per week (6 to 12 2-minute sessions with an intensity of 85-90% of maximum speed) and 70 mg / kg of Caffeine soluble with saline was injected every day for five days. Western blotting was used to evaluate p38α and HSP70 proteins. Data were analyzed using two-way analysis of variance and if a statistically significant difference was observed, Turkey test was used to determine the location of intergroup differences. Results:  HSP70 protein levels were significantly different in healthy and diabetic mice. High-intensity Interval Training and caffeine consumption significantly increased HSP70 protein compared to the supplement + diabetic group (P=0.001). exercise + supplement group had a greater effect on increasing p38α than other diabetic groups, but this difference was not significant (P=0.96).Conclusion:  High-intensity interval training and caffeine can play an effective role in counteracting damage of diabetes by increasing the expression of HSP70 in the liver.  However, high-intensity Interval Training and caffeine consumption have no effect on p38α expression in the liver of diabetic rats.
                 

کلیدواژه‌ها [English]

  • High-intensity Interval
  • Caffeine
  • diabetes
  • p38α
  • HSP70
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دوره 9، شماره 1
فروردین 1401
صفحه 83-99
  • تاریخ دریافت: 24 بهمن 1400
  • تاریخ بازنگری: 12 اردیبهشت 1401
  • تاریخ پذیرش: 15 اردیبهشت 1401
  • تاریخ اولین انتشار: 15 اردیبهشت 1401