تاثیر کوتاه مدت دو مقدار متفاوت مکمل کافئین-آب‌چغندر پیش از فعالیت هوازی شدید بر TNF-α، GDF15 و E-SELECTIN پلاسمایی در دوندگان حرفه‌ای استقامتی

نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، واحد آیت‌الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

2 دانشیار گروه فیزیولوژی ورزش، واحد آیت‌الله آملی، دانشگاه آزاد اسلامی، آمل، ایران.

3 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه گیلان، گیلان- ایران.

4 دانشیار گروه فیزیولوژی ورزش، واحد آیت‌الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

10.22049/jahssp.2022.27580.1422

چکیده

هدف: فعالیت ورزشی استقامتی شدید با افزایش التهاب، احتمال بیماری قلبی-عروقی را افزایش می‌دهد. نشان داده شده مکمل‌های حاوی نیترات قادر به مهار التهاب می‌باشد. هدف از پژوهش حاضر بررسی تاثیر مصرف دو مقدار مکمل کافئین-آب­چغندر پیش از فعالیت هوازی شدید بر سطوح سلکتین اندوتلیال (E-SELECTIN)، فاکتور تمایز رشدی-15 (GDF-15) و فاکتور نکروز دهنده تومور آلفا (TNF-α) در دوندگان حرفه‌ای ‌استقامتی بود. روش شناسی: در این مطالعه تجربی، 10 دونده ‌نخبه ‌مرد (سن 8/3±5/27، ‌وزن 8/7±9/69، شاخص ‌توده ‌بدنی 5/1±3/22 و حداکثر اکسیژن ‌مصرفی 6/4±2/52) انتخاب و به‌ صورت دوسوکور و تصادفی معکوس در سه شرایط 1، 2 و 3 به مدت هفت روز و با فاصله 2 هفته اجرا شد، مورد مطالعه قرار گرفتند؛ ۱: دوز بالا (6/9 میلی‌مول نیترات و ۴۰۰ میلی‌گرم کافئین)، ۲: دوز پایین (8/4 و ۲۰۰) و ۳: دارونما تقسیم شدند. آزمودنی‌ها روزانه ۲۵۰ سی‌سی مکمل مصرف کردند. فعالیت هوازی شدید شامل پنج کیلومتر دویدن بود. یافته‌ها: تعیین اثر تعاملی مداخله‎ها، شرایط مختلف و عامل زمان با آزمون‌های MANOVA تکراری و بونفرونی نشان داد مصرف مکمل آب چغندر-کافئین (اثر مداخله) پیش از فعالیت شدید هوازی اثر معناداری بر میزان E-SELECTIN و GDF-15 نداشته، ولی باعث کاهش TNF-α (038/0=p) شد. همچنین افزایش معنی‌داری در GDF-15 (024/0=p)، TNF-α (0001/0=p) و  E-SELECTIN (047/0=p) به همراه داشت؛ مصرف مکمل بعد از هفت روز (اثر زمان) سبب کاهش TNF-α (033/0=p)، GDF-15 (021/0=p) و  E-SELECTIN (004/0=p) پس از فعالیت ورزشی شد. نتیجه گیری: افزایش متغیرها بعد از فعالیت و کاهش آن‌ها بعد از هفت روز مکمل‌یاری نشان داد کاهش شاخص‌های التهابی، احتمالاً بر کاهش اختلال قلبی ناشی از فعالیت ورزشی شدید موثر است. 

کلیدواژه‌ها


عنوان مقاله [English]

Short-term effect of two caffeine-beetroot supplementation rate on TNF-α, GDF15 and E-SELECTIN following a course of aerobic activity in professional endurance runners

نویسندگان [English]

  • Maziar Azizi Saadatloo 1
  • Ahmad Abdi 2
  • Javad Mehrabani 3
  • Asieh Abbassi Daloii 4
1 PhD student, Department of Sports Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 Associate Professor, Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Amol, Iran.
3 Department of Sports Physiology, Faculty of Sports Sciences, University of Guilan, Guilan-Iran.
4 Associate Professor, Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
چکیده [English]

Aim: Chronic inflammation as an integral part of cardiovascular disease is associated with endothelial dysfunction. The aim of this study was to evaluate the effect of pre-intensive aerobic workout caffeine-beetroot supplementation on indicators related to vascular function including endothelial selectin (E-SELECTIN), growth differentiation factor-15 (GDF-15) and tumor necrosis factor-alpha (TNF-α) in elite  endurance runners.
Methods: In this experimental study, 10 elite male runners (age: 27.5±3.8 y, height: 176.7±7.8 cm, BMI: 22.3±1.5 kg/m2 and VO2max 52.2±4.6 ml/kg/min, were selected and with a double-blind, counterbalance, crossover and randomized design, were participated into three short-term conditions with 2 week washout. 1: high dose (9.6 mmol nitrate and 400 mg caffeine), 2: low dose (4.8 and 200) and 3: placebo. Subjects consumed 250cc of the supplement twice daily. Aerobic exercise included 5-km high intensity indoor running.
Results: intervention, conditions and time effects with MANOVA repeated measures and bonferroni statistical analysis tests showed that the short term beetroot-caffeine supplementation (intervention effect) had no significant effect on E-SELECTIN and GDF-15, but decreased TNF-α (p=0.038). Also, that increased the GDF-15 (p=0.024), TNF-α (p=0.0001) and E-SELECTIN (p=0.047) and the supplementation after seven days (time effect) decreased the levels of TNF-α (p=0.033), GDF-15 (p=0.021) and E-SELECTIN (p=0.004) following exercise. 
Conclusion: an increase in the variables after exercise and decrease after seven days of the supplementation, indicates that may improves cardiac dysfunction caused by intensive aerobic exercise.

کلیدواژه‌ها [English]

  • Caffeine
  • Nitric oxide
  • Exercise
  • Heart Diseases
  • Athlete
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دوره 9، شماره 1
فروردین 1401
صفحه 61-71
  • تاریخ دریافت: 08 دی 1400
  • تاریخ بازنگری: 09 اردیبهشت 1401
  • تاریخ پذیرش: 10 اردیبهشت 1401
  • تاریخ اولین انتشار: 10 اردیبهشت 1401