تاثیر هشت هفته تمرین مقاومتی همراه با مصرف عصاره خارخاسک بر شاخص های آنتی اکسیدانی بافت هیپوکمپِ موش های صحرایی نر درمعرض استانازول

نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I

نویسندگان

1 گروه تربیت بدنی وعلوم ورزشی، دانشگاه آزاد اسلامی، واحد محلات، محلات، ایران.

2 گروه تربیت بدنی و علوم ورزشی، دانشکده علوم ورزشی، دانشگاه اراک، اراک، ایران

10.22049/jahssp.2022.27659.1441

چکیده

هدف: امروزه بهره گیری از مواد نیروزا به معضل پیچیده­ای در ورزش تبدیل شده است، از طرفی تمرین مقاومتی و گیاه خارخاسک تاثیر گذاری زیادی در کنترل فشار اکسایشی دارد اما تعامل تاثیر تمرین مقاومتی همراه با گیاه خارخاسک و استانازول بر ظرفیت آنتی اکسیدانی کم­تر بررسی شده است. از این رو  هدف مطالعه حاضر بررسی تاثیر هشت هفته تمرین مقاومتی همراه با مصرف عصاره خارخاسک بر شاخص­های سوپر اکسیددیسموتاز (SOD)، گلوتاتیون پراکسیداز (GPX) و کاتالاز (CAT) بافت هیپوکمپِ موش­های صحرایی نر در معرض استانازول بود. روش شناسی: در این مطالعه تجربی 56 سر موش صحرایی نر با محدوده وزن 150 تا 200 گرم و میانگین سنی 8 هفته، انتخاب و در 7 گروه 8 سری شامل 1) کنترل (C)، 2) مصرف استانازول (S)، 3) مصرف استانازول همراه با mg/kg 100 خارخاسک (ST100)، 4) مصرف استانازول همراه با mg/kg 50 خارخاسک (ST50)، 5) مصرف استانازول همراه با تمرین مقاومتی (SRT)، 6) مصرف استانازول همراه با تمرین مقاومتی و mg/kg 100 خارخاسک (SRTT100) و 7) مصرف استانازول همراه با تمرین مقاومتی و mg/kg 50 خارخاسک (SRTT50) تقسیم شدند. در مدت هشت هفته گروه های 2- 7 روزانه mg/kg 5 استانازول به صورت صفاقی دریافت نمودند؛ گروه­های 5- 7 سه جلسه در هفته با شدت 30 تا 100درصد وزن بدن، تمرینات مقاومتی انجام دادند و گروه­های 3، 4، 6 و 7 روزانه دوزهای معین خارخاسک را به صورت صفاقی دریافت نمودند. برای تحلیل داده‌ها از آنالیز واریانس یک طرفه و آزمون توکی استفاده شد (05/0>p). یافته‌ها: مصرف ST سبب کاهش SOD، GPX و CAT در هیپوکمپ شد (05/0P<). با این وجود SRT باعث افزایش معناداری سطوح SOD،GPX  و CAT شد (05/0P<). همچنین ST50 و ST100  تاثیر معناداری بر افزایش سطوح SOD و GPX داشتند (05/0P<).SRTT100  و SRTT50 اثر معناداری بر افزایش سطوح SOD، GPX و CAT داشت (05/0P≤). SRTT100  اثر بیشتری بر افزایش سطوح SOD داشت (05/0P<)، در حالی که سطوح CAT در SRTT50 افزایش بیشتری داشت (05/0P<). نتیجه‌گیری: به نظر می­رسد تمرین مقاومتی و مصرف خارخاسک به طور مجزا اثرات موثری بر بهبود شاخص­های آنتی اکسیدانی دارند، با این وجود انجام تمرین مقاومتی و مصرف مکمل خارخاسک می­تواند اثرات مطلوب­تری نسبت به هر مداخله به تنهایی بر شاخص­های آنتی اکسیدانی بافت هیپوکمپ ناشی از مسمومیت استانازول داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of eight weeks of resistance training with consumption of Tribulus terrestris extract on antioxidant indices of hippocampal tissue in male rats exposed to stanazol

نویسندگان [English]

  • Behnam Shamsi 1
  • Bahram Abedi 1
  • Sajjad Ramezani 2
1 Department of Physical Education and Sport Sciences, Islamic Azad University, Mahallat Branch, Mahallat, Iran.
2 Department of Physical Education and Sports Science, Faculty of Sports Sciences, Arak University, Arak, Iran
چکیده [English]

Aim:   Today, the use of energizers has become a complex problem in sports. On the other hand, resistance training and Tribulus terrestris have a great effect on controlling oxidative stress, but the interaction of resistance training with Tribulus terrestris and Stanazol on low antioxidant capacity. More reviewed. Therefore, the aim of this study was to investigate the effect of eight weeks of resistance training with consumption of Tribulus terrestris extract on the levels of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) in the hippocampal tissue of male rats exposed to stanazol. Methods: In this experimental study, 56 male rats with a weight range of 150 to 200 g and a mean age of 8 weeks were selected and included in 7 groups of 8 series including 1) control (C), 2) stanazol (S), 3) stanazol with 100 mg / kg of tribulus terrestris (ST100), 4) Taking Stanazol with 50 mg / kg of tribulus terrestris (ST50), 5) Taking Stanazol with resistance training (SRT), 6) Taking Stanazol with resistance training and 100 mg / kg of tribulus terrestris (SRTT100) and 7) Taking Stanazol with training Resistance and 50 mg / kg of tribulus terrestris (SRTT50) were divided. For eight weeks, the 2-7 groups received 5 mg / kg daily of stanazole peritoneally;  Groups 5-7 performed resistance exercises three sessions a week with an intensity of 30 to 100% of their body weight and groups 3, 4, 6 and 7 received certain doses of tribulus terrestris daily.  One-way analysis of variance with Tukey post hoc test was used to analyze the findings (p <0.05). Results: Consumption of S had a significant effect on reducing SOD, GPX and CAT levels in rat hippocampal tissue (P<0.05), however, SRT significantly increased SOD, GPX and CAT levels (P<0.05), as well as ST50 and ST100. They had a significant effect on increasing SOD and GPX levels (P<0.05). SRTT100 and SRTT50 had a significant effect on increasing SOD, GPX and CAT levels (P<0.05). SRTT100 had a greater effect on increasing SOD levels (P<0.05), while CAT levels had a greater increase in SRTT50 (P<0.05).Conclusions:  It seems that resistance training and consumption of tribulus terrestris separately have effective effects on improving antioxidant indices, however, resistance training and consumption of tribulus terrestris supplementation can have more favorable effects than any intervention alone on Have antioxidant properties of hippocampal tissue due to stanazole poisoning.

کلیدواژه‌ها [English]

  • resistance training
  • Hippocampus
  • Antioxidant
  • Stanazol
  • Tribulus terrestris
1.             Hernández-Guerra AI, Tapia J, Menéndez-Quintanal LM, Lucena JS. Sudden cardiac death in anabolic androgenic steroids abuse: case report and literature review. Forensic sciences research. 2019;4(3):267-73.
2.             Galderisi M, Cardim N, D’andrea A, Bruder O, Cosyns B, Davin L, et al. Atlet kalbine çoklu modaliteli kardiyak görüntüleme yaklaþýmý: Avrupa Kardiyovasküler Görüntüleme Derneði Uzman Uzlaþýsý. TÜRK KARDÝYOLOJÝ DERNEÐÝ ARÞÝVÝ. 2016;44(Supp: 1):1-21.
3.             Marocolo M, Katayama PL, Meireles A, Barbosa Neto O. Combined effects of exercise training and high doses of anabolic steroids on cardiac autonomic modulation and ventricular repolarization properties in rats. Canadian journal of physiology and pharmacology. 2019;97(12):1185-92.
4.             Tabor J, Collins R, Debert CT, Shultz SR, Mychasiuk R. Neuroendocrine Whiplash: Slamming the Breaks on Anabolic-Androgenic Steroids Following Repetitive Mild Traumatic Brain Injury in Rats May Worsen Outcomes. Frontiers in neurology. 2019 May 8;10:481.
5.             Kaldur T, Unt E, Ööpik V, Zilmer M, Eha J, Paapstel K, et al. The acute effects of passive heat exposure on arterial stiffness, oxidative stress, and inflammation. Medicina. 2016;52(4):211-6.
6.             Nasiru S, Bulama I, Abdurrahman J, Abubakar N, Salisu A, Salisu B, et al. Neurobiochemical Roles of Low Molecular Weight Antioxidants on Oxidative Stress Biomarkers and Severity of Ischemic Stroke in Wistar Rats. J NeurolNeurolDisord. 2018;4(1):101-11.
7.             Olher RR, Rosa TS, Souza LHR, Oliveira JF, Soares BRA, Ribeiro TBA, et al. Isometric Exercise Improves Redox Balance and Blood Pressure in Hypertensive Adults. Medicine and science in sports and exercise. 2019Nov.21:10
8.             Katoli M, Daloii AA. The effect of Boldenone and Aerobic Training with Jujube Extract and Gallic Acid on Glutathione Peroxidase and Catalase in Heart Tissue of Male Wistar Rats. Traditional and Integrative Medicine. 2017(2)1:15-23.
9.             Habibpoor Karimabadi F, Abbasi Daloii A, Abdi A, Ziaolhagh S. Structural changes of cardiac tissue in response to L-carnitine supplementation during endurance training in Wistar male rats toxicated by steroid anabolic hormone. Hormozgan Medical Journal. 2017;21(2):86-95. [In Persian]
10.          Azizbeigi K, Azarbayjani MA, Atashak S, Stannard SR. Effect of moderate and high resistance training intensity on indices of inflammatory and oxidative stress. Research in sports medicine. 2015;23(1):73-87. [In Persian]
11.          López-Lluch G. Physiological Aspects of Coenzyme Q10 in Plasma in Relationship with Exercise and Aging.  Nutrition and Functional Foods for Healthy Aging: Elsevier; 2017. p. 307-16.
12.          Vaidya VV, Kondalkar PL, Shinde MA, Gotmare SR. Optimization of extraction protocol for isolation of biomarkers with known anti-diabetic potential from fruits of Tribulus terrestris L. Journal of Pharmacognosy and Phytochemistry. 2018;7(5):45-51.
13.          Khanahmadi, V., Samadi, A., Hasan Pour Ezati, M. Comparing the Effect of Air Pollution on Salivary Malondialdehyde and Total Antioxidant Capacity Response to a Bangsbo Protocol in Indoor vs. Outdoor Environment in Male Adolescent Futsal Players. Journal of Applied Health Studies in Sport Physiology, 2020; 7(2): 36-44. [In Persian]
14.          Roozbehi M, Gaeini A, Nouri R, Kordi MR. Interaction effect of stanozolol and endurance training on oxidant and antioxidant capacity in liver tissue of healthy male wistar rats. Studies in Medical Sciences. 2019;30(7):537-47. [In Persian]
15.          Dehghan F, Hajiaghaalipour F, Yusof A, Muniandy S, Hosseini SA, Heydari S, et al. Saffron with resistance exercise improves diabetic parameters through the GLUT4/AMPK pathway in-vitro and in-vivo. Scientific reports. 2016 Apr 28;6(1):1-2. [In Persian]
16.          Arjmand A, Abedi B, Hosseini SA, Ramezani S. The Effect of Resistance Training with Tribulus terrestris Extract on Apoptosis of Heart Tissue in Rats. Journal of North Khorasan University of Medical Sciences. 2021;13(2):70-6. [In Persian]
17.          Papageorgiou CD, Stamatopoulos VP, Samaras CD, Statharakos NS, Papageorgiou ED, Dzhambazova EB. Hormesis-Like Benefits of Physical Exercises Due To Increased Reactive Oxygen Species. Physical Education, Sport, Kinesitherapy Research Journal/PESKRJ. 2016;1(3):76-84. [In Persian]
18.          Sollanek KJ, Burniston JG, Kavazis AN, Morton AB, Wiggs MP, Ahn B, et al. Global proteome changes in the rat diaphragm induced by endurance exercise training. PLoS One. 2017;12(1):1-21
19.          Dornelles GL, Bueno A, de Oliveira JS, da Silva AS, França RT, da Silva CB, et al. Biochemical and oxidative stress markers in the liver and kidneys of rats submitted to different protocols of anabolic steroids. Molecular and cellular biochemistry. 2017;425(1-2):181-9.
20.          Seyed A, Farsi S, Hosseini SA, Kaka G. Antioxidant effects of swimming training and curcumin in withdrawal period of alcohol overdose in rats. Journal of Fasa University of Medical Sciences. 2018;8(3):901-10. [In Persian]
21.          Sadowska-Krępa E, Kłapcińska B, Jagsz S, Nowara A, Szołtysek-Bołdys I, Chalimoniuk M, et al. High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver. Pharmacological Reports. 2017;69(4):673-8.
22.          Kwon TD, Lee MW, Kim KH. The effect of exercise training and water extract from propolis intake on the antioxidant enzymes activity of skeletal muscle and liver in rat. Journal of exercise nutrition & biochemistry. 2014;18(1):9.
23.          rozbehi m, kordi m, nouri r, gaeini a. interaction effect of stanozolol and endurance training on oxidant and antioxidant capacity in liver tissue of healthy male wistar rats. Urmia Medical Journal. 2019;30(7):537-47. [In Persian]
24.          Call JA, Donet J, Martin KS, Sharma AK, Chen X, Zhang J, et al. Muscle-derived extracellular superoxide dismutase inhibits endothelial activation and protects against multiple organ dysfunction syndrome in mice. Free Radical Biology and Medicine. 2017;113:212-23.
25.          Kormanovski A, Castillo-Hernández MDC, Guevara-Balcázar G, Pérez T, Lara-Padilla E. Gender differences in nitric oxide and antioxidant response to physical stress in tissues of trained mice. Physiol Pharmac. 2019.
26.          Enriquez-del-Castillo LA, Candia-Lujan R, Moreno-Brito V, Fierro LGDL, Reza-López SA, González-Rodríguez E, et al. Sistema antioxidante, actividad física y consumo máximo de oxígeno en adultos jóvenes. Biotecnia. 2019;21(2):91-6.
27.          Najafi H, Firouzifar MR, Shafaat O, Ashtiyani SC, Hosseini N. Protective effects of Tribulus terrestris L extract against acute kidney injury induced by reperfusion injury in rats. Iranian journal of kidney diseases. 2014;8(4):292-298. [In Persian]
28.          Hamid S, Jamil A, Rashid A, Aziz Q, Aslam M. EFFECT OF TRIBULUS TERRESTRIS ON SERUM LUTEINIZING HORMONE IN SPRAGUE DAWLEY RATS. Pakistan Journal of Physiology. 2017;13(4):38-40.
29.          Alzahrani S, Ezzat W, Elshaer R, Abd El-Lateef A, Mohammad H, Elkazaz A, et al. Standarized Tribulus terrestris extract protects against rotenone-induced oxidative damage and nigral dopamine neuronal loss in mice. Journal of Physiology and Pharmacology. 2018;69(6).980-994
30.          Kovac JR, Pan M, Arent S, Lipshultz LI. Dietary adjuncts for improving testosterone levels in hypogonadal males. American journal of men's health. 2016;10(6):NP109-NP17.
31.          Simioni C, Zauli G, Martelli AM, Vitale M, Sacchetti G, Gonelli A, et al. Oxidative stress: role of physical exercise and antioxidant nutraceuticals in adulthood and aging. Oncotarget. 2018;9(24):17181.
32.          Ahmadi M, Abbassi-Daloii A, Ziaolhagh SJ, Yahyaei B. Structural changes of cardiac tissue in response to boldenone supplementation with or without alcoholic extract of jujuba fruit during resistance training in male Wistar rats. Feyz Journal of Kashan University of Medical Sciences. 2018;21(6). [In Persian]
33.          Yin L, Wang Q, Wang X, Song L-N. Effects of Tribulus terrestris saponins on exercise performance in overtraining rats and the underlying mechanisms. Canadian journal of physiology and pharmacology. 2016;94(11):1193-201.
 
دوره 9، شماره 1
فروردین 1401
صفحه 48-60
  • تاریخ دریافت: 11 بهمن 1400
  • تاریخ بازنگری: 06 اردیبهشت 1401
  • تاریخ پذیرش: 09 اردیبهشت 1401
  • تاریخ اولین انتشار: 09 اردیبهشت 1401