اثر هشت هفته تمرین تداومی و تناوبی همراه با مصرف وارفارین بر بیان ژن استئوپونتین بافت قلب و شاخص‌های انعقادی در رت‌های نر اسپراگو-دوالی با انفارکتوس میوکارد

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد تهران جنوب، تهران، ایران

2 دانشیار گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد تهران جنوب، تهران، ایران

3 استادیار گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد تهران جنوب، تهران، ایران

4 استادیار گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد اسلامشهر، تهران، ایران

10.22049/jahssp.2022.27515.1403

چکیده

هدف: انفارکتوس میوکارد علاوه بر تخریب رمودلینگ بافت قلبی، سیستم هموستاز خون را نیز تحت تاثیر قرار می‌دهد. هدف از مطالعه حاضر بررسی تاثیر هشت هفته تمرین تداومی و تناوبی همراه با مصرف وارفارین بر بیان ژن استئوپونتین بافت قلب و شاخص‌های انعقادی (PT، PTT، INR) در رت‌های نر با انفارکتوس میوکارد می‌باشد. روش‌ پژوهش: در این مطالعه تجربی، 42 سر رت (هشت هفته‌ای) به طور تصادفی به هفت گروه شش‌تایی کنترل سالم، انفارکتوس میوکارد، انفارکتوس میوکارد همراه با (تمرین تناوبی، تمرین تداومی، وارفارین، تمرین تناوبی+‌وارفارین و تمرین تداومی+‌وارفارین) تقسیم شدند. مدت تمرینات تناوبی فزاینده (یک دقیقه: 16-33، دو دقیقه‌: 10-14) متر بر دقیقه و تداومی فزاینده (14-24 متر بر دقیقه، 10-60 دقیقه) هشت هفته، پنج جلسه در هفته بود. القا با ﺗﺰرﻳﻖ زیرپوستی ایزوپروترنول در دو دوز 85 mg/kg ایجاد شد. دوز مصرفی وارفارین 5/0 میلی‌گرم بر کیلوگرم وزن بدن در روز بود. 48 ساعت بعد از آخرین جلسه تمرین، نمونه‌های خونی و بافت قلب برای اندازه‌گیری متغیرها استخراج شدند. برای تجزیه و تحلیل داده‌ها نیز از آزمون‌های T مستقل، آنوا یک راهه و تحلیل واریانس دو عاملی استفاده شد (5 0/0>P). یافته‌ها: القای انفارکتوس میوکارد منجر به افزایش بیان ژن استئوپونتین (001/0P=)، مقادیر PT (001/0P=)، PTT (001/0P=) و INR (001/0P=) نسبت به گروه کنترل سالم می‌شود. نتایج آزمون توکی نشان داد مقادیر PT در گروه انفارکتوس میوکارد‌+وارفارین (002/0P=) و گروه انفارکتوس میوکارد+‌وارفارین+‌ECT‌ (001/0P=)، کاهش معنی‌داری نسبت به گروه انفارکتوس میوکارد داشت. کاهش معنی‌داری در مقادیر PTT در تمام گروه‌ها (5 0/0>P) و همچنین افزایش معنی‌داری در INR گروه‌های آزمایشی که وارفارین دریافت کرده بودند نسبت به گروه انفارکتوس میوکارد مشاهده شد (05/0>P). نتیجه‌گیری: در انفارکتوس میوکارد، انجام تمرینات تناوبی و تداومی در کنار مصرف وارفارین قادر به کنترل فاکتورهای انعقادی PT و PTT است. مطالعات بیشتری در رابطه با تغییر استئوپونتین با روش‌های درمانی مطالعه حاضر نیاز است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of eight weeks of continuous and interval training with warfarin on expression of cardiac osteopontin gene and coagulation indices in male Sprague-Dawley rats with myocardial infarction

نویسندگان [English]

  • Tooran Abbasi 1
  • Abdolali Banaeifar 2
  • Sajad Arshadi 3
  • Yaser Kazemzadeh 4
1 Ph.D Student in Sports Physiology, Islamic Azad University, South Tehran Branch, Tehran, Iran.
2 Associate Professor, Department of Sports Physiology, Islamic Azad University, South Tehran Branch, Tehran, Iran.
3 Assistant Professor, Department of Sports Physiology, Islamic Azad University, South Tehran Branch, Tehran, Iran.
4 Assistant Professor, Department of Sports Physiology, Islamic Azad University, Islamshahr Branch, Tehran, Iran.
چکیده [English]

purpose: Myocardial infarction, in addition to destroying cardiac tissue remodeling, also affects the blood homeostasis system. The purpose of the present study is to investigate the effect of eight weeks of continuous and interval training with warfarin on expression of cardiac osteopontin gene and coagulation indices (PT, PTT, INR) in male rats with myocardial infarction. Methods: In this experimental study, 42 male rats (eight weeks old) were randomly divided to seven groups of (six in each) healthy control, myocardial infarction, myocardial infarction‌ with (interval training, ‌continuous training, ‌warfarin, ‌interval training‌+‌warfarin, and ‌continuous training+‌warfarin). Duration of incremental interval training (one minute: 16-33, two minute: 10-14) m/min and incremental continues training (14-24 m/min, 10-60 minutes) was eight weeks, five sessions per week. Induction was induced by subcutaneous injection of isoproterenol in two doses of 85 mg‌/‌kg. 48 hours after the last training session, blood samples and heart tissue were extracted to measure the variables. Independent T-test, one-way ANOVA and Two-way analysis of variance were used to analyze the data (P<0.05). Results: Induction of myocardial infarction leads to increased expression of osteopontin (P=0.001), PT values (P=0.001), PTT (P=0.001) and INR (P=0.001) compared to the healthy control group. The results of Tukey test showed that PT values in myocardial infarction‌+warfarin group (P=0.002) and myocardial infarction+warfarin+ECT group (P=0.001) had a significant decrease compared to myocardial infarction group. There was a significant decrease in PTT values in of all groups (P<0.05) and also a significant increase in INR of the experimental groups that had received warfarin compared to the myocardial infarction group (P<0.05). Conclusions: In myocardial infarction, interval and continuous training along with warfarin can control PT and PTT coagulation factors. Further studies on the replacement of osteopontin with therapeutic modalities of the present study are needed.

کلیدواژه‌ها [English]

  • Myocardial infarction
  • Isoproterenol
  • Exercise
  • Warfarin

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

مراجع

  1.  

    1. Mc Namara K, Alzubaidi H, Jackson JK. Cardiovascular disease as a leading cause of death: how are pharmacists getting involved? Integrated pharmacy research & practice. 2019;8:1.
    2. Johansson S, Rosengren A, Young K, Jennings E. Mortality and morbidity trends after the first year in survivors of acute myocardial infarction: a systematic review. BMC Cardiovascular Disorders. 2017;17(1):1-8.
    3. Hashmi S, Al-Salam S. Acute myocardial infarction and myocardial ischemia-reperfusion injury: a comparison. International journal of clinical and experimental pathology. 2015;8(8):8786.
    4. Alabaf yousefi F, Pouzesh Jadidi R, BASHIRI J, Azali Alamdari K, Vakili J. The Effects of HIIT and Curcumin Supplementation on CD31+ Capillary Cell Count and the Expression of VEGF and MMP9 of Left Ventricle in Isoproterenol Induced Myocardial Infraction Model Rats %J Journal of Sport Biosciences. 2021;13(2):179-94.
    5. Gustafsson N, Ahlqvist JB, Näslund U, Wester P, Buhlin K, Gustafsson A, et al. Calcified carotid artery atheromas in panoramic radiographs are associated with a first myocardial infarction: a case-control study. Oral surgery, oral medicine, oral pathology and oral radiology. 2018;125(2):199-204. e1.
    6. Singh A, Gill G, Kaur H, Amhmed M, Jakhu H. Role of osteopontin in bone remodeling and orthodontic tooth movement: a review. Progress in orthodontics. 2018;19(1):1-8.
    7. Lorenzen JM, Martino F, Scheffner I, Bröcker V, Leitolf H, Haller H, et al. Fetuin, matrix-Gla protein and osteopontin in calcification of renal allografts. PLoS One. 2012;7(12):e52039.
    8. Mohammadi Moghaddam A, Behpoor N, Nazari A. Pre-treatment effects of continuous and intermittent endurance training on gene expression of myocardial Osteopontin in male Wistar rats following the induction of myocardial infarction. yafteh. 2019;21(2).
    9. Dunkley JC, Irion CI, Yousefi K, Shehadeh SA, Lambert G, John-Williams K, et al. Carvedilol and exercise combination therapy improves systolic but not diastolic function and reduces plasma osteopontin in Col4a3−/− Alport mice. American Journal of Physiology-Heart and Circulatory Physiology. 2021;320(5):H1862-H72.
    10. Holbrook AM, Pereira JA, Labiris R, McDonald H, Douketis JD, Crowther M, et al. Systematic overview of warfarin and its drug and food interactions. Archives of internal medicine. 2005;165(10):1095-106.
    11. Price PA, Faus SA, Williamson MK. Warfarin causes rapid calcification of the elastic lamellae in rat arteries and heart valves. Arteriosclerosis, thrombosis, and vascular biology. 1998;18(9):1400-7.
    12. Krüger T, Oelenberg S, Kaesler N, Schurgers LJ, van de Sandt AM, Boor P, et al. Warfarin induces cardiovascular damage in mice. Arteriosclerosis, thrombosis, and vascular biology. 2013;33(11):2618-24.
    13. Schurgers LJ, Joosen IA, Laufer EM, Chatrou ML, Herfs M, Winkens MH, et al. Vitamin K-antagonists accelerate atherosclerotic calcification and induce a vulnerable plaque phenotype. 2012.
    14. Khan HA, Alhomida AS, Al Rammah TY, Sobki SH, Ola MS, Khan AA. Alterations in prothrombin time and activated partial thromboplastin time in patients with acute myocardial infarction. International journal of clinical and experimental medicine. 2013;6(4):294.
    15. Jones DA, Wright P, Alizadeh MA, Fhadil S, Rathod KS, Guttmann O, et al. The use of novel oral anticoagulants compared to vitamin K antagonists (warfarin) in patients with left ventricular thrombus after acute myocardial infarction. European Heart Journal-Cardiovascular Pharmacotherapy. 2021;7(5):398-404.
    16. Yagi T, Naito T, Kato A, Hirao K, Kawakami J. Association Between the Prothrombin Time–International Normalized Ratio and Concomitant Use of Antibiotics in Warfarin Users: Focus on Type of Antibiotic and Susceptibility of Bacteroides fragilis to Antibiotics. Annals of Pharmacotherapy. 2021;55(2):157-64.
    17. Sobhani V, Mohammadi M, Shirvani H, Amini A. Long-Term Effect of High-Intensity Interval and Concurrent Exercise on Blood Coagulation and Fibrinolysis Parameters in Non-Athlete Healthy Young Men. The Horizon of Medical Sciences. 2016;22(4):329-36.
    18. Moholdt T, Aamot IL, Granøien I, Gjerde L, Myklebust G, Walderhaug L, et al. Aerobic interval training increases peak oxygen uptake more than usual care exercise training in myocardial infarction patients: a randomized controlled study. Clinical rehabilitation. 2012;26(1):33-44.
    19. Dun Y, Thomas RJ, Smith JR, Medina-Inojosa JR, Squires RW, Bonikowske AR, et al. High-intensity interval training improves metabolic syndrome and body composition in outpatient cardiac rehabilitation patients with myocardial infarction. Cardiovascular diabetology. 2019;18(1):1-11.
    20. Ebadi B, Damirchi A, Alamdari K, Darbandi-Azar A, Naderi N. Cardiomyocyte mitochondrial dynamics in health and disease and the role of exercise training: A brief review. 2018;7(3):107-15.
    21. Majidi A, Poozesh Jadidi R, Nourazar MAR, Bashiri J, Azali Alamdari KJSP. Effects of Aerobic Training and Curcumin Supplementation on Cardiomyocyte Apoptosis and MiRNAs Expression in Rats Exposed to Arsenic. 2020;12(48):39-60.
    22. Moieni A, Hosseini SA. Effect of Resistance Training Combined with Curcumin Supplementation on Expression of Regulatory Genes Related to Myocardial Remodeling in Obese Rats %J Journal of Applied Health Studies in Sport Physiology. 2020;7(2):45-52.
    23. Rohani H, Azali Alamdari K, Helali zadeh M. Effect of Aerobic Training on Overall Metabolic Risk and Indices Levels in Patients with Metabolic Syndrome: A Meta-Analysis Study %J Sport Physiology. 2016;8(31):17-44.
    24. Smith J. Effects of strenuous exercise on haemostasis. British journal of sports medicine. 2003;37(5):433-5.
    25. San Jose MCZ, Apaga NEP, Florento L, Gan RN. Effects of aerobic exercise and training on coagulation, platelet aggregation, and plasma lipids. Vascular Disease Prevention. 2005;2(2):145-50.
    26. Lekakis J, Triantafyllidi H, Galea V, Koutroumbi M, Theodoridis T, Komporozos C, et al. The immediate effect of aerobic exercise on haemostatic parameters in patients with recently diagnosed mild to moderate essential hypertension. Journal of thrombosis and thrombolysis. 2008;25(2):179-84.
    27. Menzel K, Hilberg T. Coagulation and fibrinolysis are in balance after moderate exercise in middle-aged participants. Clinical and Applied Thrombosis/Hemostasis. 2009;15(3):348-55.
    28. Bakhtiyari A, Gaeni A, Chobineh S, Kordi MR, Hedayati M. Effect of 12-weeks high-intensity interval training on SIRT1, PGC-1α and ERRα protein expression in aged rats. Journal of Applied Health Studies in Sport Physiology. 2018;5(2):95-102.
    29. Li L, ZHANG Lk, PANG Yz, PAN Cs, QI Yf, Chen L, et al. Cardioprotective effects of ghrelin and des‐octanoyl ghrelin on myocardial injury induced by isoproterenol in rats 1. Acta Pharmacologica Sinica. 2006;27(5):527-35.
    30. Mohajeri D, Monadi A, Mousavi G, Rezaei Saber A. Cardioprotective effect of resveratrol on isoproterenol-induced experimental myocardial infarction in rat. Veterinary Clinical Pathology The Quarterly Scientific Journal. 2014;8(3 (31) Autumn):537-48.
    31. Eizadi M, Soory R, Ravasi A, Baesy K, Choobineh S. Relationship between TCF7L2 Relative Expression in Pancreas Tissue with Changes in Insulin by High Intensity Interval Training (HIIT) in Type 2 Diabetes Rats. SSU_Journals. 2017;24(12):981-93.
    32. Hadi H, Gaeini A, MOTAMEDI P, Rajabi H. @ THE EFFECT OF AEROBIC TRAINING ON CARDIAC EXPRESSION OF MIR-126 IN DIABETIC AND HEALTHY RATS. 2016.
    33. Zhang X, Zhang X, Wang X, Zhao M. Influence of andrographolide on the pharmacokinetics of warfarin in rats. Pharmaceutical biology. 2018;56(1):351-6.
    34. Ganjkhani L, Osali A. The Effect of 4 week resistance training on male rat hepatic ABCA1 protein plasma HDL-C levels. Journal of Applied Health Studies in Sport Physiology. 2019;6(1):22-30.
    35. Abdelaziz Mohamed I, Gadeau A-P, Hasan A, Abdulrahman N, Mraiche F. Osteopontin: A promising therapeutic target in cardiac fibrosis. Cells. 2019;8(12):1558.
    36. Kong P, Christia P, Frangogiannis NG. The pathogenesis of cardiac fibrosis. Cellular and molecular life sciences. 2014;71(4):549-74.
    37. Patel M, Rodriguez D, Yousefi K, John-Williams K, Mendez AJ, Goldberg RB, et al. Osteopontin and LDLR Are Upregulated in Hearts of Sudden Cardiac Death Victims With Heart Failure With Preserved Ejection Fraction and Diabetes Mellitus. Frontiers in Cardiovascular Medicine. 2020;7.
    38. Shimodaira T, Matsuda K, Uchibori T, Sugano M, Uehara T, Honda T. Upregulation of osteopontin expression via the interaction of macrophages and fibroblasts under IL-1b stimulation. Cytokine. 2018;110:63-9.
    39. Singh M, Dalal S, Singh K. Osteopontin: At the cross-roads of myocyte survival and myocardial function. Life sciences. 2014;118(1):1-6.
    40. Baghaiee B, Karimi P, Siahkouhian M, Pescatello LS. Moderate aerobic exercise training decreases middle-aged induced pathologic cardiac hypertrophy by improving Klotho expression, MAPK signaling pathway, and oxidative stress status in Wistar rats. Iranian journal of basic medical sciences. 2018;21(9):911.
    41. Jafari M, Yekrangi Z, Marhamati M, Reyhani M, Karimi H. Acute effects of aerobic and anaerobic activities on blood adipokines and vascular adhesive molecules in young women. Journal of applied health studies in sport physiology. 2020;7(1):65-72.
    42. Li J, Yousefi K, Ding W, Singh J, Shehadeh LA. Osteopontin RNA aptamer can prevent and reverse pressure overload-induced heart failure. Cardiovascular research. 2017;113(6):633-43.
    43. MirHeidari L, SOURI R, RAVASI AA. Effect continuous training and soya supplement on the expression of the mir-29 gene in the heart of ovariectomized rats.
    44. Pu J, Ding S, Ge H, Han Y, Guo J, Lin R, et al. Efficacy and safety of a pharmaco-invasive strategy with half-dose alteplase versus primary angioplasty in ST-segment–elevation myocardial infarction: EARLY-MYO Trial (Early Routine Catheterization After Alteplase Fibrinolysis Versus Primary PCI in Acute ST-Segment–Elevation Myocardial Infarction). Circulation. 2017;136(16):1462-73.
    45. Kawai M, Harada M, Motoike Y, Koshikawa M, Ichikawa T, Watanabe E, et al. Impact of serum albumin levels on supratherapeutic PT-INR control and bleeding risk in atrial fibrillation patients on warfarin: a prospective cohort study. IJC Heart & Vasculature. 2019;22:111-6.
    46. Mega JL, Simon T. Pharmacology of antithrombotic drugs: an assessment of oral antiplatelet and anticoagulant treatments. The Lancet. 2015;386(9990):281-91.
    47. Pouzesh Jadidi G, Seifi-Skishahr F, Bolboli L, Azali Alamdari K, Pourrahim Ghouroghch A. Effect of high intensity interval training and curcumin supplementation on left ventriclular miR-133 and miR-1 gene expression levels in isoproterenol induced myocardial infarction rat model %J Journal of Practical Studies of Biosciences in Sport. 2021:-.
    48. Ohkura N, Oishi K, Atsumi G-i. Blood coagulation and metabolic profiles in middle-aged male and female ob/ob mice. Blood Coagulation & Fibrinolysis. 2015;26(5):522-6.
    49. Dallak M, Bin-Jaliah I, Sakr HF, Al-Ani B, Haidara MA. Swim exercise inhibits hemostatic abnormalities in a rat model of obesity and insulin resistance. Archives of physiology and biochemistry. 2019;125(1):79-84.
    50. Azali Alamdari K. Effects of 8 weeks of high intensity interval and moderate intensity continuous training on serum ICAM-1, CRP and cardiometabolic risk factors in middle-agedmen %J Journal of Practical Studies of Biosciences in Sport. 2018;6(12):83-101.
    51. Kingsley JD, Figueroa A. Acute and training effects of resistance exercise on heart rate variability. Clinical physiology and functional imaging. 2016;36(3):179-87.
    52. Bolboli L, Sattari M, Hakimi V. Effect of High Intensity Interval Training and Moderate Intensity Continuous Training on Electrocardiographic Indices in Sedentary Men %J Journal of Applied Health Studies in Sport Physiology. 2020;7(2):53-8.
    53. El-Sayed MS. Effects of exercise on blood coagulation, fibrinolysis and platelet aggregation. Sports medicine. 1996;22(5):282-98.
    54. Alizade M. Relationship between aerobic exercise activity with the blood cell count parameters (CBC) and factors of associated with iron metabolism and PT (prothrombin time) and PTT (minor thromboplastin time) tests in non-athletic men and women. 2019.
    55. Habibi M, Torkaman G, Goosheh B, Hedayati M. Effects of aerobic and combined resistance-aerobic training on the coagulation factors of young healthy men. Physiol Pharmacol. 2009;13(1):98-107.
    56. Posthuma JJ, van der Meijden PE, ten Cate H, Spronk HM. Short-and Long-term exercise induced alterations in haemostasis: a review of the literature. Blood reviews. 2015;29(3):171-8.
    57. Amini A, Sobhani V, Mohammadi MT, Shirvani H. Acute effects of aerobic, resistance and concurrent exercises, and maximal shuttle run test on coagulation and fibrinolytic activity in healthy young non-athletes. The Journal of sports medicine and physical fitness. 2017;57(5):633-42.
    58. Barnes GD, Kong X, Cole D, Haymart B, Kline‐Rogers E, Almany S, et al. Extended International Normalized Ratio testing intervals for warfarin‐treated patients. Journal of Thrombosis and Haemostasis. 2018;16(7):1307-12.
    59. Evans A, Perez Ii, Yu G, Kalra L. Should stroke subtype influence anticoagulation decisions to prevent recurrence in stroke patients with atrial fibrillation? Stroke. 2001;32(12):2828-32.
    60. Albertsen IE, Rasmussen LH, Overvad TF, Graungaard T, Larsen TB, Lip GY. Risk of stroke or systemic embolism in atrial fibrillation patients treated with warfarin: a systematic review and meta-analysis. Stroke. 2013;44(5):1329-36.
    61. Tripodi A, Mannucci PM. The coagulopathy of chronic liver disease. New England Journal of Medicine. 2011;365(2):147-56.
    62. Kujovich JL. Coagulopathy in liver disease: a balancing act. Hematology 2014, the American Society of Hematology Education Program Book. 2015;2015(1):243-9.
    63. Kilic L, Yildiz I, Sen FK, Erdem MG, Serilmez M, Keskin S, et al. D-dimer and international normalized ratio (INR) are correlated with tumor markers and disease stage in colorectal cancer patients. Cancer Biomarkers. 2015;15(4):405-11.
    64. Loforte A, Fiorentino M, Gliozzi G, Mariani C, Folesani G, Suarez SM, et al., editors. Heart transplant and hepato-renal dysfunction: the model of end-stage liver disease excluding international normalized ratio as a predictor of postoperative outcomes. Transplantation proceedings; 2019: Elsevier.
    65. Farhoudi M, Rad SF, Mahmoudi J, Sadigh-Eteghad S, Roozbeh M, Singh RJ, et al. Effect of Chronic Stress on Coagulation Parameters in Warfarin-Treated Male Rats. Journal of Experimental and Clinical Neurosciences. 2020;7(1).
    66. Gashi AI, Zivkovic V, Gjorgoski I, Gontarev S, Azemi A. Original Article Regular physical activity may influence stress hormone cortisol in Wistar rats. Journal of Physical Education and Sport. 2020.
مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 8، شماره 2
مهر 1400
صفحه 70-82

فایل ها

سابقه مقاله

  • تاریخ دریافت: 23 آبان 1400
  • تاریخ بازنگری: 07 دی 1400
  • تاریخ پذیرش: 11 دی 1400

هم رسانی

ارجاع به این مقاله

آمار