تأثیر 12 هفته تمرینات ورزشی هوازی بر روی رفتارهای افسردگی و سطوح هورمون‌های تستوسترون و کورتیکوسترون در رت‌های نر مبتلا به آلزایمر

نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I

نویسندگان

1 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، تهران، ایران

2 کارشناسی ارشد فیزیولوژی ورزشی، گروه فیزیولوژی ورزش، دانشگاه پیام نور، تهران، ایران

10.22049/jahssp.2021.27472.1399

چکیده

هدف: با توجه به نقشی که هورمون‌ها در میزان افسردگی ناشی از بیماری آلزایمر دارند و همچنین تاثیری که تمرینات ورزشی بر سطوح ترشح هورمون‌ها در بدن دارند، هدف از پژوهشحاضر بررسی تأثیر 12 هفته تمرینات ورزشی هوازی بر روی رفتارهای افسردگی و سطوح هورمون‌های تستوسترون و کورتیکوسترون در رت­های نر مبتلا به آلزایمر بود. روش شناسی: بدین منظور 40 سر رت نر ویستار با میانگین سنی 8 هفته‌ و با میانگین وزنی 33±237 گرم بطور تصادفی در چهار گروه 10 سری شامل: کنترل، ورزش، آلزایمر و ورزش +آلزایمر قرار گرفتند. برای القا آلزایمر، مقدار 3 میلی‌گرم بر کیلوگرم استرپتوزوتوسین در حجم 5 میکرولیتر آب مقطر استریل در ناحیه بطن مغز تزریق شد. پروتکل تمرینی به مدت 12 هفته، پنج جلسه در هفته و 30 دقیقه در هر جلسه با سرعت 10 متر بر دقیقه از هفته اول تا ششم و سرعت 12 متر بر دقیقه از هفته هفتم تا دوازدهم اجرا شد. در انتهای دوره تمرینی آزمون‌‌های ترجیح ساکارز و شنای اجباری به عنوان تست‌های برآورد افسردگی انجام شد. پس از تکمیل تست‌های رفتاری، اندازه‌گیری سطوح هورمون‌های تستوسترون و کورتیکوسترون به روش الایزا انجام شد. یافته‌ها: نتایج نشان داد که 12 هفته تمرینات ورزشی سبب کاهش معنی‌دار زمان بی‌تحرکی در تست شنای اجباری و افزایش معنی‌دار آزمون ترجیح ساکارز (001/0=P) در رت‌های مبتلا به آلزایمر شده است. همچنین یک دوره تمرینات ورزشی هوازی سبب افزایش معنی دار هورمون‌ تستوسترون (006/0=P) و کاهش معنی‌دار هورمون کورتیکوسترون (006/0=P) در رت‌های مبتلا به آلزایمر شده است. نتیجه‌گیری: به نظر می‌رسد که تمرین هوازی دویدن روی تردمیل، با افزایش سطوح تستوسترون و کاهش کورتیکوسترون بر رفتارهای مربوط به افسردگی در موش های صحرایی مبتلا به بیماری آلزایمر موثر بوده است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of 12 weeks of aerobic exercise training on depressive behaviors, testosterone and corticosterone levels in rats with Alzheimer's

نویسندگان [English]

  • Saeed Naghibi 1
  • Ali Barzegari 1
  • Mozhgan Rostami 2
1 Assistant Professor, Department of physical education, Payame Noor University, Tehran, IRAN.
2 MSc, Department of physical education, Payame Noor University, PO BOX 19395-3697, Tehran, IRAN
چکیده [English]

Aim: Considering the role of hormones on depression's levels that caused by Alzheimer's disease, and also the effect of exercise training on hormone secretion levels in the body, the aim of this study was to investigate the effect of 12 weeks of aerobic exercise training on depressive behaviors and hormones levels of testosterone and corticosterone in Alzheimer's male rats. Methods: For this purpose, 40 male Wistar rats with an average age of 8 weeks and average weight of 237±33 g were randomly divided into four groups of 10: control, exercise, Alzheimer's and exercise + Alzheimer's. To induce Alzheimer's, 3 mg/kg streptozotocin in 5 μl of sterile distilled water was injected into the ventricular region. The training protocol was performed for 12 weeks, 5 sessions/week and 30 minutes in each session at a speed of 10 m/min from the first to the 6 weeks and 12 m/min from the 7 to the 12 weeks. At the end of the training period, Sucrose preference and forced swimming tests were performed as tests for estimating depression. After completing the behavioral tests, testosterone and corticosterone levels were measured by ELISA. Results: The results showed that 12 weeks of exercise training significantly reduced the immobility time in the forced swimming test (P = 0.001)  and significantly increased the sucrose preference test (P = 0.001) in rats with Alzheimer's disease. Also, a period of exercise training has caused a significant increase in testosterone (P = 0.006) and a significant decrease in corticosterone (P = 0.006) in rats with Alzheimer's disease. Conclusion: Aerobic exercise training on treadmill appears to be effective in treating depressive behaviors in rats with Alzheimer's disease by increasing testosterone levels and decreasing corticosterone.

کلیدواژه‌ها [English]

  • Alzheimer
  • depression
  • testosterone
  • Corticosterone
  • Aerobic exercise training
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