اثر تعاملی تمرین‌هوازی و مکمل کورکومین بر بیان ژن NRF-1 و NRF-2 میتوکندری در کاردیومیوسیت‌های‌ موش‌های صحرایی نر مدل سکته قلبی

نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

2 استادیار، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

3 استادیار، گروه دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

4 استادیار، گروه تربیت بدنی، مرکز آموزش علوم پایه، دانشگاه علوم انتظامی امین، تهران، ایران

5 دانشیار فیزیولوژی ورزشی، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

10.22049/jahssp.2021.27321.1370

چکیده

هدف: تحقیق حاضر با هدف بررسی اثر تعاملی تمرین­هوازی و مصرف مکمل کورکومین بر بیان­ژنNRF-1  و NRF-2 میتوکندریایی کاردیومیوسیت‌ موش‌های صحرایی نر مدل سکته قلبی انجام شد. روش شناسی: 32 سر موش صحرایی نر به صورت تصادفی به چهار گروه کنترل، تمرین­هوازی، مصرف مکمل و­ تمرین­هوازی + مصرف مکمل تقسیم شدند. گروه‌های تمرین و تمرین + مکمل به مدت 8 هفته در برنامه تمرین­هوازی شرکت کردند. تمرین روی نوارگردان الکترونیکی هوشمند حیوانی به صورت 5 روز در هفته انجام شد و در این زمان گروه کنترل و مصرف مکمل برنامه تمرینی نداشتند. همچنین، مکمل کورکومین پنج روز در هفته و به صورت گاواژ به گروه‌های مکمل و تمرین + مکمل داده شد. پس از جراحی، بیان­ژن‌هایNRF-1  و NRF-2 کاردیومیوسیت با استفاده از روش  Real-time PCRبه­دست آمد. برای تجزیه و تحلیل ﺩﺍﺩه‌ها ﺁﺯﻣﻮن‌های شاپیرو-ویلک و تحلیل واریانس دو­طرفه مورد استفاده قرار گرفت. یافته‌ها: هر دو متغیر تمرین و مکمل کورکومین به صورت معنی­داری بیان ­ژن‌های NRF-1 و NRF-2 کاردیومیوسیت‌ها را افزایش دادند. همچنین نتایج نشان داد که بین این دو متغیر اثر تعاملی مثبت و معنی‌داری وجود دارد، به طوری که افزایش بیان ­ژن‌های NRF-1 و NRF-2 در گروه تمرین + مکمل از سایر گروه‌ها بیشتر بود. نتیجه‌گیری: به نظر می­رسد هر دو عامل تمرین و مصرف مکمل کورکومین به تنهایی و در تعامل با هم می­توانند باعث افزایش بیان ژن برخی عوامل محرک بیوژنز میتوکندریایی در کاردیومیوسیت‌ موش‌های نر مبتلا به سکته قلبی شوند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Interactive Effect of Aerobic Training and Curcumin Supplementation on Mitochondrial Cardiomyocyte Nrf1 and Nrf2 Gene Expression in Myocardial infraction Male Rat Model

نویسندگان [English]

  • Alireza Nikoozadeh 1
  • Roghayeh Pouzesh Jadidi 2
  • Mir Alireza Nourazar 3
  • Masoud Asgharpour Arshad 4
  • Jabbar Bashiri 5
1 PHD student of Exercise Physiology, Department of Physical Education, Tabriz branch, Islamic Azad University, Tabriz, Iran
2 Assistant Prof., Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran
3 Assistant Prof., Department of Vet, Tabriz branch, Islamic Azad University, Tabriz, Iran
4 Assistant Prof. Department of Physical Education, Basic Sciences Education Center, Amin University of Law Enforcement Sciences, Tehran, Iran
5 Associate Prof., Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran
چکیده [English]

Aim: The aim of this study was to investigate the interactive effect of aerobic training and curcumin supplementation on  mitochondrial NRF-1 and NRF-2 gene expression of cardiomyocytes in infarction model male rats. Methods: 32 male rats were randomly divided into four groups: control, aerobic training, supplementation, and aerobic training + supplementation.  The training  and training + supplementation groups participated in an aerobic training program for 8 weeks. The training was performed on an intelligent animal electronic treadmill 5 days a week, and at this time the control and supplementation  groups did not have an training program. Also, curcumin supplementation was given to the supplementation and training + supplementation groups five days a week by gavage.  After surgery, the expression of NRF-1 and NRF-2  genes in cardiomyocytes was avaluated using real-time PCR method. Shapiro-Wilk and two-way analysis of variance were used for the analysis of data. Results: Both training and curcumin supplementation variables significantly increased the expression of NRF-1 and NRF-2 in cardiomyocytes.  The results also showed that there is a positive and significant interaction between two variables, so that the increase in expression of NRF-1 and NRF-2 genes in the training + supplement group was more than the other groups. Conclusions:  It seems that both training and curcumin supplementation alone and in interaction with each other can increase the gene expression of some factors that stimulate mitochondrial biogenesis in cardiomyocytes of male rats with myocardial infarction.

کلیدواژه‌ها [English]

  • aerobic training
  • curcumin
  • Myocardial infarction
  • mitochondrial biogenesis
  • NRF-1
  • NRF-2
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