تاثیر تمرین با شدت های مختلف بر شاخص منتخب آپوپتوزی در بافت کبد رت های نر نژاد ویستار

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I


1 گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور کرج ،البرز،ایران

2 استاد فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.

3 استادیار فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، پژوهشگاه تربیت بدنی و علوم ورزشی، تهران، ایران



هدف: کبد یکی از اندام­های حیاتی بدن است که نقش بسیار مهمی در متابولیسم مواد، سم زدایی داروها و دفع مواد مضر از بدن دارد و نارسایی­های کبدی به عنوان یکی از خطرات جدی سلامتی بشر محسوب می­شوند. آپوپتوز یکی از ویژگی­های برجسته بیماری­های کبد است. پیشرفت بیماری کبد تحت تاثیر تعادل بین توانایی­های آپوپتوزی و ضد آپوپتوزی قرار دارد. هدف پژوهش کنونی، بررسی تغییرات شاخص منتخب آپوپتوز در بافت کبد رت­های نر ویستار ناشی از انجام سه پروتکل تمرین ورزشی بود. روش شناسی: در این مطالعه تجربی، 32 سر رت (8 هفته­ای) به طور تصادفی به چهار گروه هشت تایی کنترل، تمرین هوازی با شدت متوسط، هوازی پرشدت و تمرین هوازی تناوبی پرشدت تقسیم شدند. مدت تمرینات هشت هفته، پنج جلسه در هفته بود. 24 ساعت بعد از آخرین جلسه تمرین، نمونه­های بافت بطن کبد استخراج شد و در دمای 80- درجه سانتی گراد تا زمان آزمایش نگهداری شد. برای آزمون فرضیه­های پژوهش از آزمون ANOVA یک طرفه و برای مقایسه بین گروهی از آزمون تعقیبی توکی استفاده شد.  یافته‌ها: نتایج نشان داد که نسبت به گروه کنترل، بیان ژن 21P در هر سه گروه تمرین هوازی با شدت متوسط، هوازی پرشدت و تمرین هوازی تناوبی پرشدت به طور معنی­داری کاهش یافته است (001/0P≤)، به طوری به ترتیب  به میزان 0018/0 واحد، 0017/0 واحد و 001/0 واحد نسبت به گروه کنترل کاهش داشته است. نتیجه‌گیری: نتایج نشان داد پروتکل­های ورزشی پژوهش کنونی اثرات یکسانی برکاهش بیان ژن 21P در بافت کبد دارند. بنابراین، می­تواند به عنوان یک سازوکار پیشگیرانه جهت جلوگیری و یا کاهش آپوپتوز بافت کبدی مورد توجه قرار گیرد.


عنوان مقاله [English]

Effect of exercise with different intensities on the selected index of apoptosis in the liver tissue of male Wistar rats

نویسندگان [English]

  • Soodabeh Ghanbari 1
  • Ali Asghar Ravasi 2
  • Mohammad Shariatzade Joneidi 3
1 Master of science Karaj Payame Noor University ,Karaj , Iran
2 Professor of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
3 Assistant Professor of Exercise Physiology, Department of Exercise Physiology, Sport Sciences Research Institute, Tehran, Iran
چکیده [English]

Aim: The liver is one of the vital organs of the body that plays a very important role in metabolism, detoxification of drug, excretion of harmful substances from the body, and liver failure is considered as one of the serious risks to human health. Apoptosis is one of those noticeable feature of liver disease. Liver disease increasing affected by the balance between apoptotic and anti-apoptotic abilities. The aim of the present study was to investigate the changes in the selected index of apoptosis the liver tissue of male Wistar rats resulting from three exercise training protocols. Methods: In This experimental study, Thirty-two rats )8 weeks) were divided into four groups of eight: control, Moderate Intensity Training (MIT), High Intensity training (HIT), High intensity interval training (HIIT). The training lasted eight weeks, five sessions per week. 24 hours after the last training session, liver ventricular tissue samples were extracted and stored at -80 ° C until the experiment. One-way ANOVA test was used to test the research hypotheses and Tukey post-hoc test was used for group comparison. Results: The results showed that compared to the control group, P21 gene expression was significantly reduced in all three groups of MIT, HIT and HIIT ( P≤0/001) So that it has decreased by 0.0018 units, 0.0017 units and 0.001 units, respectively, compared to the control group. Conclusions: The results showed that the sports protocols of the current study have the same effects on reducing the expression of P21 gene in liver tissue. Therefore, it can be considered as a preventive mechanism to prevent or reduce liver apoptosis.

کلیدواژه‌ها [English]

  • gene expression
  • Liver tissue
  • apoptosis
  • Protein P21
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