تاثیر دو نوع تمرین تناوبی با شدت بالا و تداومی با شدت متوسط بر سطوح TNF-α و IL-10 سرمی در رت های نر چاق

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه گیلان، رشت، ایران

2 استاد فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی ،دانشکده علوم ورزشی، دانشگاه گیلان، رشت، ایران.

3 دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه گیلان، رشت، ایران.

4 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکده تربیت بدنی، دانشگاه آزاد اسلامی واحد هشتگرد، هشتگرد، ایران.

چکیده

هدف: هدف پژوهش حاضر، بررسی آثار تمرینات ورزشی تناوبی با شدت بالا (HIIT) و تداومی با شدت متوسط (MICT) بر فاکتورهای التهابی (TNF-α و IL-10 ) در رت‌های نر چاق بود.روش شناسی:  40 سر رت نر در دو گروه به مدت 10 هفته تحت رژیم غذایی پرچرب (32 سر) و رژیم غذایی استاندارد (8 سر) قرار گرفتند. پس از القاء چاقی، 8 سر رت از گروه رژیم غذایی پرچرب (O) و 8 سر رت گروه رژیم غذایی استاندارد (C) قربانی شده و سایر رت‌های چاق به‌ طور تصادفی به 3 گروه شاهد چاق (OC)، تمرین تداومی با شدت متوسط (MICT) و تمرین تناوبی با شدت بالا (HIIT) تقسیم شدند. پروتکلHIIT شامل اجرای 10 مرحله فعالیت 4 دقیقه‌ای با شدت معادل 85 تا 90 درصد VO2max و با دوره‌های استراحتی فعال 2 دقیقه‌ای و پروتکل MICT با شدت معادل 65 تا 70 درصد  VO2maxبا مسافت طی شده همسان با پروتکل HIIT به مدت 12 هفته و 5 جلسه در هر هفته اجرا شدند. نمونه­های سرمی برای اندازه‌گیری سطوح سرمی TNF-α و IL-10 به روش الایزا جمع‌آوری شد. یافته‌ها: در هر دو گروه HIIT  و MICT نسبت به گروه OC سطوح سرمی  TNF-α بطور معنی داری کاهش و سطوح سرمی IL-10 به صورت معنی‌داری افزایش یافت (05/0P≤). اما در مقایسه بین گروه‌های HIIT  و MICT  در سطوح سرمی TNF-α و IL-10، تفاوت معنی‌داری مشاهده نشد. نتیجه‌گیری:به نظر می‌رسد که هر دو پروتکل تمرینی HIIT و MICT منجر به بهبود التهاب مزمن به واسطه کاهش TNF-α وافزایش IL-10 می‌شوند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Two Types of High Intensity and Moderate Intensity Continuous Training on Serum Levels of TNF-a and IL-10 in Obese Male Rats

نویسندگان [English]

  • Amir Akbari 1
  • Hamid Mohebbi 2
  • Mousa Khalafi 3
  • Kamilia Moghaddami 4
1 PhD Student in Exercise Physiology, Department of Sports Physiology, Faculty of Sports Sciences, University of Guilan, Rasht, Iran.
2 Professor of Exercise Physiology, Department of Sports Physiology, Faculty of Sports Sciences, University of Guilan, Rasht, Iran.
3 PhD in Exercise Physiology, Department of Sports Physiology, Faculty of Sports Sciences, University of Guilan, Rasht, Iran.
4 Assistant Professor of Exercise Physiology, Department of Sports Science, Faculty of Physical Education, Islamic Of Azad University, Hashtgerd , Hashtgerd, Iran.
چکیده [English]

Aim:The purpose of this study was to investigate the effects of high intensity interval training (HIIT) and moderate intensity continuous training (MICT) on inflammatory factors (TNF-α and IL-10) in obese male rats.  Methods: Forty male rats were divided into two groups: high-fat diet (n=32) and standard diet (n=8), for 10 weeks. After inducing obesity, eight rats from the high-fat diet group (O) and eight from the standard dietary group (C) were sacrificed, and the other obese rats were randomly divided into three groups: obesity control (OC), moderate intensity continuous training (MICT) and high intensity interval training (HIIT). The HIIT protocol, including 10 bouts of 4-minute activity with equivalent intensity of 85-90% vo2max and 2 minute active rest periods, and the MICT protocol with equivalent intensity of 65-70% VO2max, with covered distance matched to that of the HIIT protocol, were performed for 12 weeks and 5 sessions per week. Serum samples were collected to measure serum levels of TNF-α and IL-10 by ELISA.  Results: In both HIIT and MICT groups, serum levels of TNF-α were significantly decreased and serum levels of IL-10 were significantly increased compared to OC group (p < 0.05). Conclusions: But there was no significant difference between the HIIT and MICT groups in serum levels of TNF-α and IL-10. It seems that both HIIT and MICT training protocols improve chronic inflammation by decreasing TNF-α and increasing IL-10.

کلیدواژه‌ها [English]

  • High Intensity Interval Training
  • Moderate Intensity Continuous Training
  • Alpha Tumor Necrosis Factor
  • Interleukin 10
  • Obesity

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 6، شماره 1
فروردین 1398
صفحه 86-93

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سابقه مقاله

  • تاریخ دریافت: 18 فروردین 1398
  • تاریخ بازنگری: 12 تیر 1398
  • تاریخ پذیرش: 25 تیر 1398

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