پاسخ تغییرات وزن، هورمون ضد اشتهای سرم و میزان فعالیت آنزیم آنتی‌اکسیدان عضلۀ اسکلتی موش‌های نر نژاد ویستار به یک دوره مکمل سازی با ال-آرژنین همراه با تمرین استقامتی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان ، ایران

2 دانشجوی کارشناسی ارشد فیزیولوژی ورزشی کاربردی، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان ، ایران

چکیده

مقدمه‌و‌هدف: هدف از مطالعه حاضر بررسی تاثیر 8هفته تمرین هوازی فزاینده با مصرف مکمل ال-آرژنین بر تغییرات-وزن، فعالیت آنزیم سوپر‌اکسید‌دیسموتاز عضلۀ اسکلتی و سطوح سرمی هورمون لپتین در موش‌های‌صحرایی نر بود.
مواد‌ و روش کار: 32سر موش صحرایی نر ویستار، به صورت تصادفی در 4گروه(کنترل، تمرین،ال-آرژنین وتمرین+ال-آرژنین)تقسیم شدند. پروتکل تمرین هوازی شامل دویدن بر روی نوارگردان به مدت 8 هفته(5جلسه در هفته)بود. آب و غذای استاندارد به صورت آزاد در اختیار آزمودنی‌ها قرار گرفت و در گروه های مکمل ال-آرژنین، میزان 4 گرم ال-آرژنین در هر 100میلی‌لیتر آب مصرفی استفاده شد. 48 ساعت پس از آخرین جلسۀ تمرینی و 8ساعت ناشتایی شبانه، آزمودنی‌ها تشریح شدند و نمونه‌های خونی و بافتی از آزمودنی‌ها جمع‌آوری شد و غلظت هورمون لپتین با استفاده از روش الایزا و میزان فعالیت آنزیم سوپر‌اکسید‌دیسموتاز با استفاده از روش اسپکتروفتومتری اندازه‌گیری شد. برای تجزیه‌وتحلیل داده‌ها از آزمون تحلیل واریانس یک‌طرفه و آزمون تعقیبی توکی استفاده شد.
یافته‌ها: نتایج نشان داد پس از 8 هفته تمرین به همراه مصرف مکمل ال-آرژنین تفاوت معناداری در میزان فعالیت آنزیم سوپر‌اکسید‌یسموتاز عضلۀ اسکلتی در بین گروه‌ها وجود ندارد(0.05<P)، اما میزان لپتین در گروه تمرین+ال-آرژنین نسبت به گروه‌های تمرین و ال-آرژنین به طور معناداری افزایش یافته بود(0.05>P). همچنین پس از 8 هفته تغییرات وزن گروه تمرین+ال-آرژنین نسبت به گروه کنترل به طور معناداری کمتر بود(0.05>P).
نتیجه‌گیری: با توجه به تاثیر مکمل ال-آرژنین همراه با تمرین بر افزایش سطح لپتین خون، شاید بتوان عنوان کرد که مکمل ال-آرژنین مانع از افت لپتین خون و عوارض ناشی از آن در ورزشکاران خواهد شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Weight changes, serum anti-anxiety hormone, and the enzymatic activity of anti-oxidant enzyme of the skeletal muscle in male Wistar rats in response to an L-arginine supplementation with a period endurance training

نویسندگان [English]

  • Agha Ali Ghasemnian 1
  • Samaneh Hadi 2
1 Associate professor, Department of Sport Science, Faculty of Humanities, University of Zanjan, Zanjan ,Iran
2 Master Student in Applied Exercise Physiology , Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran.
چکیده [English]

Background & Objective: The purpose of this study, was to investigate the effect of 8weeks incremental aerobic exercise training with L-arginine supplementation on Muscular Enzymatic activity of superoxide dismutase and serum leptine in adult male wistar rat. Materials & Methods:32 mature male Wistar rats, were randomly divided into 4 groups: (control, incremental aerobic training, Incremental aerobic training+L-Arginine and L-Arginine). The training protocol consisted of running on treadmill for 8 weeks (5day/week). Water and food were freely available for samples. In the L-Arginine supplement groups, 4 gr of L-Arginine were used per 100 ml of water. Then,48 hours after the last exercise session and after 8 hours of fasting, blood and tissue samples were collected and the concentration of leptin hormone was measured by ELISA method and the enzyme activity of super-oxide dismutase in the muscle was measured by spectrophotometric method. Data were analyzed using one-way ANOVA and Tukey's post hoc test. Results: The results showed that after 8 weeks of incremental aerobic exercise training with L-Arginine supplementation there was no significant difference in Muscular Enzymatic activity of superoxide dismutase Between groups (P> 0.05). but, serum leptin levels in the exercise with L-Arginine supplementation group significantly increased compared to exercise and L-Arginine groups (P <0.05). Also after 8 weeks, weight change in exercise with L-Arginine supplementation group were significantly lower than control group (P <0.05). Discussion and conclusion: Regarding the effect of L-arginine supplementation with exercise on increasing blood leptin levels, it may be concluded that L-arginine supplementation prevents blood leptin loss and complications in athletes

کلیدواژه‌ها [English]

  • تمرین هوازی فزاینده
  • آنتی‌اکسیدان
  • فشار اکسایشی
  • بالانس انرژی
 

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