تاثیر شش هفته لیگاسیون عصب نخاعی بر سطوح mRNA کاینزین-1 در عصب سیاتیک موش‌های صحرایی نر

نوع مقاله: مقاله پژوهشی

نویسندگان

1 عضو هیئت علمی گروه علوم ورزشی دانشگاه ولی عصر(عج) رفسنجان.

2 گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد یزد

چکیده

مقدمه: انتقال آکسونی فرایند حیاتی در سیستم عصبی بوده که نورون و پایانه‌های عصبی را از طریق تهیه پروتئین‌ها، چربی‌ها و میتوکندری و پاک کردن پروتئین‌های تاخورده برای جلوگیری از ایجاد سمیت، حفظ می‌کند. کاینزین-1 یکی از پروتئین های درگیر انتقال آکسونی بوده که در انتقال سریع رو به جلو درگیر است. باتوجه به رایج بودن اختلال پروتئین‌های درگیر در انتقال آکسونی در بیماری های تخریب عصب، هدف این مطالعه بررسی اثر فعالیت کاهش یافته و درد نوروپاتیک بر بیان ژن کاینزین-1 عصب سیاتیک رت های نر ویستار بود. مواد و روش‌ها: 10سر موش صحرایی نر نژاد ویستار با میانگین وزن30±250 گرم به دوگروه کنترل سالمو گروه کاهش فعالیتتقسیم شدند. طی 6 هفته پس از آن آزمون‌های رفتاری درد نوروپاتیک در گروه‌های پژوهشی به طور مستمر انجام شد. در پایان هفته ششم تغییرات بیان ژن کاینزین-1 در عصب سیاتیک با تکنیکReal time  اندازه‌گیری محاسبه شد. نتایج: پس از 6 هفته، آزمون‌های رفتاری درد نوروپاتیک آلوداینیای مکانیکی و پردردی حرارتی نشان داد که در گروهکاهش فعالیتآستانه درد نسبت به گروه کنترل به طور معناداری کمتر بود(05/0>P). همچنین میزان بیان ژن کاینزین-1 در عصب سیاتیک در گروهکاهش فعالیتشده به طور معناداری نسبت به گروه کنترل افزایش یافته بود(05/0>P). نتیجه گیری: به نظر می‌رسد فعالیت بدنی کاهش‌یافته و درد نوروپاتیک با بیان ژن افزایش‌یافته کاینزین-1 در فیبر عصبی سیاتیک مرتبط است. با توجه به اعمال فیزیولوژیک کاینزین-1 در نورونها احتمالاً این شرایط موجب اختلالات عملکردی سیستم عصبی - عضلانی می‌شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effects of Six Weeks of Spinal Nerve Ligation on Sciatic Nerve Keynesin-1 Gene Expression in Male Rats

نویسندگان [English]

  • Zahra Mahdavi Jafari 1
  • Niloufar Shojaie 2
1 Faculty member of Vali-e-Asr University of Medical Sciences, Rafsanjan.
2 Department of Sport Physiology, Islamic Azad University, Yazd Branch
چکیده [English]

Background & Objectives: Axonal transmission is a vital process in nervous system that protects neuron and nervous terminals by providing proteins, fats and mitochondria and clearing unfolded proteins to prevent cell toxicity. Keynesin-1 is one of involved proteins in axonal transmission that participates in rapid forward transmission. Therefore, considering the prevalence of dysfunction in proteins associated with axonal transmission in many of neurodegenerative diseases, the of this research was to investigate the effects of decreased physical activity and neuropathic pain on keynesin-1 expression of in male Wistar rats sciatic nerve. Materials & Methods: 10 male Wistar rats (weight: 250±30 gr) were randomly divided into control (C) and decreased physical activity groups (SNL). Through six weeks, neuropathic pain behavior tests were conducted continually in both groups. At the end of the six weeks, the changes in keynesin-1 gene expression in sciatic nerve were measured with Real Time PCR method. Results: The behavioral tests demonstrated that spinal nerve ligation induced thermal hyperalgesia and mechanical allodynia in the SNL group. Decreased pain threshold was observed throughout the study (p<0.05). Additionally, in comparison with the C group, keynesin-1 gene expression in sciatic nerve fibers was significantly higher in the SNL group (p<0.05). Conclusion: It seems that decreased physical activity and neuropathic pain is associated with up regulated keynesin-1 gene expression in sciatic nerve. With respect to keynesin-1 physiologic functions in the nerves, this conditions can likely lead to dysfunction of neuromuscular system.

کلیدواژه‌ها [English]

  • Decreased Physical Activity
  • Keynesin-1
  • Sciatic
 
 

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