بررسی تأثیر هشت هفته تمرین تناوبی با شدت بالا و تمرین استقامتی با شدت متوسط همراه با مکمل دهی کوئرستین بر بیان ژن‌ PLIN2 و ATGL کبد موش های صحرایی چاق دیابتی

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 گروه مدیریت ورزشی، دانشکده علوم تربیتی و روان شناسی، دانشگاه محقق اردبیلی، اردبیل، ایران

10.22049/jahssp.2024.29164.1604

چکیده

هدف: چاقی باعث التهاب مزمن شده و التهاب موجب بروز مقاومت انسولینی در بیماری دیابت نوع 2 (T2DM) می­گردد. بدین ترتیب، هدف از مطالعه حاضر بررسی تأثیر هشت هفته تمرین تناوبی با شدت بالا و تمرین استقامتی با شدت متوسط همراه با مکمل­دهی کوئرستین بر بیان ژن­ PLIN2 و ATGL کبد موش­های صحرایی ­چاق دیابتی بود. روش شناسی: چهل و دو سر موش صحرایی نر ویستار با 8 هفته سن تهیه و تعداد 36 سر از این حیوانات پس از 8 هفته رژیم غذایی پرچرب مورد القای دیابت قرار گرفتند. موش­­های صحرایی به گروه­های زیر تقسیم­بندی شدند (6n=): سالم کنترل، دیابت کنترل، دیابت مکمل کوئرستین، دیابت تمرین تناوبی با شدت بالا (HIIT)، دیابت تمرین تداومی با شدت متوسط (MICT)، دیابت تمرین HIIT به همراه مکمل کوئرستین و دیابت تمرین MICT به همراه مکمل کوئرستین. برای دو گروه تمرینی 8 هفته تمرین HIIT با و بدون مکمل کوئرستین و برای دو گروه دیگر 8 هفته تمرین MICT با و بدون مکمل کوئرستین روی نوار گردان اجرا شد. 72 ساعت پس از آخرین جلسه تمرین برای بررسی بیان ژن­های PLIN2 و ATGL بافت کبد جدا شد. از آزمون تحلیل واریانس دو راهه با آزمون تعقیبی توکی (با سطح معناداری 05/0>P) برای بررسی تفاوت بین گروه­های تمرینی استفاده شد. یافته‌ها: پس از 8 هفته مداخله تنها برای میزان بیان ژن ATGL در بین چهار گروه تمرینی تفاوت معنادار دیده شد (001/0=P)، به­طوری که تمرین HIIT و MICT همراه با مصرف کوئرستین به ترتیب با سطح معناداری (001/0=P) و (015/0=P) باعث افزایش بیشتر سطوح آن نسبت به دو گروه تمرینی دیگر شده بود. نتیجه‌گیری: ظاهراً اجرای هر دو نوع تمرین بر بیان ژن ATGL و PLIN2 اثرگذار بوده است و هنگامی که تمرینات به­همراه مصرف کوئرستین باشند این مکمل می­تواند با کنترل سطوح خون به­صورت غیر مستقیم بر بیان ژن ATGL در شرایط بیماری T2DM تأثیرگذار باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigating the effect of eight weeks of high-intensity interval training and moderate-intensity continuous training with quercetin supplementation on the PLIN2 and ATGL gene expression in the liver of diabetic obese rats

نویسندگان [English]

  • Aydin Valizadeh 1
  • Mojdeh Khajehlandi 1
  • Raha Mohammadi 1
  • Hamed Kheirollahi 2
1 Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
2 Department of Sport Management, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
چکیده [English]

Aim: Obesity causes chronic inflammation, and inflammation causes insulin resistance in type 2 diabetes mellitus (T2DM). Thus, the aim of the present study was to investigate the effect of eight weeks of high-intensity interval training and moderate-intensity continuous training with quercetin supplementation on the gene expression of PLIN2 and ATGL in the liver of diabetic obese rats.
Methods: Forty two male Wistar rats at 8 weeks of age were purchased, and 36 of these animals were induced with T2DM after 8 weeks of high-fat diet. The rats were divided into the following groups (n=6): healthy control, diabetic control, diabetic quercetin supplementation, high-intensity interval training with diabetes (HIIT), moderate-intensity continuous training with diabetes (MICT), HIIT training with quercetin, and MICT training with quercetin. For one training group, 8 weeks of HIIT training and for the other 8 weeks of MICT training on the treadmill were performed. Seventy two hours after the last training session, liver tissues was isolated to check gene expression of PLIN2 and ATGL. Two-way analysis of variance with Tukey's post-hoc test (with a significance level of P<0.05) was used to analyze the data.
Results: After 8 weeks of intervention, a significant difference was seen only in the level of ATGL gene expression between four training groups (P=0/001), so that in HIIT and MICT training groups with quercetin its levels was statistically more than two other training groups with the significant levels of (P=0/001) and (P=0/015), respectively. Conclusions: Apparently, the implementation of both types of exercise has an effect on ATGL and PLIN2 gene expression, and when exercise is combined with quercetin, this supplementation can indirectly affect ATGL gene expression in T2DM conditions by controlling blood levels.

کلیدواژه‌ها [English]

  • Moderate intensity continuous exercise
  • high intensity intermittent exercise
  • liver
  • type 2 diabetes mellitus

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 11، شماره 1
فروردین 1403
صفحه 237-250

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  • تاریخ دریافت: 19 آبان 1402
  • تاریخ بازنگری: 29 دی 1402
  • تاریخ پذیرش: 30 دی 1402

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