تأثیر هشت هفته تمرین مقاومتی به همراه مکمل‌یاری اسپیرولینا بر مسیر پیام‌رسانی Wnt/ GSK-3β / TSC2/ S6K بافت کلیه رت های نر

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه خلیج فارس، بوشهر، ایران.

2 گروه فیزیولوژی ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه خلیج فارس، بوشهر، ایران

3 دانشیار، گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه خلیج فارس، بوشهر، ایران

چکیده

هدف: سیگنالینگ Wnt یکی از مهم­ترین مسیرهای پیام­رسانی در­گیر در جریان تمرینات مقاومتی بوده که تاثیر مولکولی زیادی در مسیر تکاملی بافت کلیه و بیماری­های این بافت دارد. هدف از تحقیق حاضر بررسی تاثیر هشت هفته تمرین مقاومتی به همراه مکمل‌یاری اسپیرولینا بر مسیر پیام­رسانیWnt/ GSK-3β / TSC2/ S6K  بافت کلیه رت­های نر بود. روش شناسی: پژوهش حاضر از نوع طرح تجربی بود که تعداد 32 سر رت نر جوان با 3 ماه سن و میانگین وزن 20±150 گرم بطور تصادفی در چهار گروه کنترل، تمرین مقاومتی، اسپیرولینا و تعامل تمرین و اسپیرولینا قرار داده­شدند. گروه­های تمرین مقاومتی به به مدت هشت هفته و 5 جلسه در هفته به تمرین مقاومتی پرداختند که تمرین روزانه شامل 3 ست 5 تکراری با 1 دقیقه استراحت بین هر تکرار و 2 دقیقه استراحت بین ست­ها بود و بار تمرین از 30 درصد وزن بدن هر رت در هفته اول شروع و به 100 درصد وزن در هفته پایانی رسید. گروه­های مکمل، مکمل اسپیرولینا را با دوز 200 میلی­گرم به ازای هر کیلو­گرم وزن بدن به مدت هشت هفته مصرف کردند. برای بیان ژن­های مورد مطالعه تحقیق از روش Real-Time PCR استفاده شد. از نرم افزار SPSS نسخه 24 و آمار استنباطی تحلیل آنووای دوطرفه جهت تجزیه و تحلیل داده­ها استفاده شد. یافته‌ها: تغییرات بیان ژن Wnt، GSK-3β و TSC2 در هیچ یک از گروه­ها در مقایسه با گروه کنترل معنا­دار نبود (P≥0.05). در گروه تعاملی تمرین مقاومتی و مکمل اسپیرولینا افزایش معنا­داری در میزان بیان ژن S6K درمقایسه با گروه کنترل مشاهده شد (017/0= P، 511/6F=). نتیجه‌گیری: با توجه  به عدم تاثیر مکمل اسپیرولینا بر سیگنالینگ Wnt به نظر می­رسد استفاده از این مکمل تاثیر منفی بر بافت کلیه نمی­گذارد، هر­چند با توجه به محدود بودن تحقیقات، بررسی­های بیشتر بر روی بافت کلیه بخصوص در نمونه­های بیمار توصیه می­شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of eight weeks of resistance training and Spirulina platensis supplementation on the signaling pathway of Wnt-GSK3β-TSC2-S6K in the kidney tissue of male rats

نویسندگان [English]

  • Hamid Reza Sadeghipour 1
  • Fatemeh Raeisi 2
  • Abdossalehleh zar 3
1 Department of Sport Sciences, School of Literature and Humanities, Persian Gulf University, Boushehr, Iran.
2 Department of Sport Sciences, School of Literature and Humanities, Persian Gulf University, Boushehr, Iran
3 Associate professor, Department of Sport Science, School of Literature and Humanities, Persian Gulf University, Boushehr, Iran
چکیده [English]

Aim:      Wnt signaling is one of the most important signaling pathways involved in resistance training, which has a great molecular impact on the evolutionary path of kidney tissue. The aim of the present study was to investigate the effect of eight weeks of resistance training with spirulina supplementation on Wnt/GSK-3β/TSC2/S6K signaling pathway in the kidney tissue of male rats. Methods: In the experimental design, 32 young male rats at 3 months of age and an average weight of 150±20 grams were randomly divided into four groups: control, resistance exercise, spirulina supplement, and exercise+supplement. The resistance training groups performed the resistance exercise of climbing a ladder for eight weeks and 5 sessions per week (3 sets and 5 repetitions, 1-minute rest between repetitions and 2 minutes between sets). The intensity was 30% of the body weight in the first week and reached 100% in the final week. Spirulina in the amount of 200 mg/kg/day was added to the water of the supplementary groups. 24 hours after the last training session, kidney tissue was removed and the expression level of the dependent variables was measured using the REAL TIME-PCR method. Cross-sectional analysis of variance was used to analyze the data at a significant level of 0.05. Results: Changes in Wnt, GSK-3β and TSC2 gene expression were not significant in any of the groups compared to the control group (P≥0.05). In the interactive group of resistance exercise and Spirulina supplement, a significant increase in S6K gene expression was observed in comparison with the control group (P=0.017, F=6.511). Conclusion: Considering the lack of effect of Spirulina supplement on Wnt signaling, it seems that the use of spirulina does not have a negative effect on the kidney tissue, although due to the limited research, more investigations especially in the kidney patients are recommended.

کلیدواژه‌ها [English]

  • Resistance Training
  • Spirulina
  • Wnt Signaling

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 11، شماره 1
فروردین 1403
صفحه 223-236

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  • تاریخ دریافت: 17 آبان 1402
  • تاریخ بازنگری: 30 دی 1402
  • تاریخ پذیرش: 30 دی 1402

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