تاثیر تمرین تناوبی با شدت بالا بر بیان پروتئین های CHOP و ATF6 بافت کبد در رت های مبتلا به دیابت نوع2

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشگاه آزاد اسلامی، واحد علوم و تحقیقات ، گروه تربیت بدنی و علوم ورزشی، تهران، ایران.

2 دانشیار، گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات، علوم انسانی و اجتماعی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی ، تهران، ایران

3 گروه فیزیولوژی ورزشی دانشکده تربیت بدنی دانشگاه خوارزمی تهران ایران

4 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات، علوم انسانی و اجتماعی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران

10.22049/jahssp.2023.28973.1586

چکیده

هدف: دیابت نوع 2 یک اختلال متابولیک پیچیده است که با مقاومت به انسولین و اختلال در تنظیم گلوکز مشخص می شود. این یک نگرانی مهم از نظر سازمان بهداشت جهانی است که میلیون ها نفر را در سراسر جهان تحت تاثیر قرار می دهد. هدف از پژوهش حاضر بررسی اثر 8 هفته تمرین تناوبی پر شدت بر بیان پروتئین های فعال کننده عامل رونویسی-6 (ATF-6) و همولوگ C/EBP   (CHOP)  بافت کبد در رت های مبتلا به دیابت نوع 2 بود. روش شناسی: پژوهش حاضر از نوع تجربی با طرح پس آزمون با گروه کنترل بود.  تعداد 24رت نر نژاد ویستار با سن 6 هفته و وزن تقریبی 200-250 گرم، از موسسه پاستور ایران تهیه شدند . رت‌ها به‌طور تصادفی به 3 گروه 8 تایی تمرین تناوبی پر شدت (HIIT) دیابتی، کنترل دیابتی و کنترل سالم تقسیم شدند. گروه‌های تمرینی در طول 8 هفته و در هر هفته، 5 جلسه تمرین HIIT  را بر روی نوار گردان اجرا کردند (پروتکل تمرین به صورت 8 هفته تمرین تناوبی، 5 جلسه در هفته با تناوب شدید 2 دقیقه ای با 2تا 8 تناوب و با 80 تا 90 درصد حداکثر سرعت دویدن و تناوب استراحت 1 دقیقه ای با 50 تا 56 درصد حداکثر سرعت دویدن، اجرا شد). پس از گذشت 48 ساعت از آخرین جلسه تمرینی و پس از اجرای روزهای ناشتایی، تمامی رت‌ها  بی‌هوش شدند و مرحله برداشت بافت کبد انجام شد. داده ها با آزمون آنالیز واریانس یک طرفه (ANOVA)  تحلیل شدند. یافته ها: نتایج نشان داد که مقادیر پروتئین ATF-6 در رت های نر دیابتی نوع 2 پس از 8 هفته تمرین تناوبی پر شدت تغییر معنی داری را نشان نداد (73/0P =). اما مقادیر پروتئین CHOP پس از 8 هفته افزایش معنی داری را نشان داد (001/0P =). مقادیر پروتئین CHOP در گروه تمرین دیابتی نسبت به گروه کنترل دیابتی کاهش غیر معنی داری را نشان داد (05/0P <)، اما در گروه تمرین دیابتی در مقایسه با گروه کنترل سالم، افزایش معنی داری را نشان داد (001/0P =). در نتیجه اجرای 8 هفته تمرین تناوبی با شدت بالا می تواند اثرات مفیدی بر کاهش استرس شبکه آندوپلاسمی در بافت کبد ناشی از دیابت نوع 2 در رت های دیابتی داشته باشد. علاوه بر این، تحقیقات بیشتری برای تایید این یافته ها و کشف مکانیسم های بالقوه درگیر، مورد نیاز است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of high-intensity interval training on the expressions of CHOP and ATF6 in liver tissue in rats with type 2 diabetes

نویسندگان [English]

  • Hadis Bayat 1
  • Mandana Gholami 2
  • hamid rajabi 3
  • Hosein abed natanzi 4
1 Department of Physical Education and Sports Science, Science and Research branch, Islamic Azad University, Tehran, Iran.
2 Associate Professor, Department of Physical Education and Sports Sciences, Faculty of Literature, Humanities and Social Sciences, Science and Research Unit, Islamic Azad University, Tehran, Iran
3 Department of Sports Physiology, Faculty of Physical Education, Kharazmi University, Tehran, Iran
4 Assistant Professor, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Aim:   Type 2 diabetes is a complex metabolic disorder characterized by insulin resistance and glucose dysregulation. It is a major concern of the World Health Organization, affecting millions of people worldwide. The purpose of this study was to investigate the effect of  8 weeks of high-intensity interval training on the expression of activating transcription factor-6 (ATF-6) and C/EBP homologue (CHOP) proteins in liver tissue in rats with type 2 diabetes. Method:  The current study was experimental with a post-test design with a control group. A number of 24 Wistar male rats, aged 6 weeks and weighing approximately 200-250 grams, were obtained from the Pasteur Institute of Iran. Rats were randomly divided into 3 groups of 8 high intensity interval training (HIIT) diabetic, diabetic control and healthy control. The training groups performed 5 HIIT training sessions on the treadmill during 8 weeks and each week (the training protocol was 8 weeks of interval training, 5 sessions per week with intense 2-minute intervals with 2-8 repetitions and 80-90 The percentage of maximum running speed and 1-minute rest intervals were performed with 50-56% of maximum running speed. After 48 hours from the last training session and after fasting days, all rats were anesthetized and liver tissue was harvested. The data were analyzed by one-way analysis of variance (ANOVA).Results: The results showed that ATF-6 protein levels in type 2 diabetic male rats did not change significantly after 8 weeks of high-intensity interval training (P = 0.73). But CHOP protein levels showed a significant increase after 8 weeks (P = 0.001). CHOP protein levels in the diabetic training group showed a non-significant decrease compared to the diabetic control group (P < 0.05), but in the diabetic training group compared to the healthy control group, it showed a significant increase (P = 0.001).  Conclusion: As a result, 8 weeks of high-intensity interval training can have beneficial effects on reducing endoplasmic reticulum stress in liver tissue caused by type 2 diabetes in diabetic rats. Furthermore, further research is needed to confirm these findings and explore the potential mechanisms involved.

کلیدواژه‌ها [English]

  • Intense interval training
  • endoplasmic reticulum stress
  • type 2 diabetes
  • obesity

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 11، شماره 1
فروردین 1403
صفحه 86-110

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  • تاریخ دریافت: 07 مهر 1402
  • تاریخ بازنگری: 08 مهر 1402
  • تاریخ پذیرش: 17 مهر 1402

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