اثر طول دوره مصرف کورکومین به همراه تمرینات HIIT بر تعادل آنتی‌اُکسیدانی عضلۀ اسکلتی، قلب و کبد رت‌های نر

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان، ایران

2 گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان

3 گروه فیزیولوژی ورزشی، دانشگاه تربیت مدرس، تهران، ایران

10.22049/jahssp.2023.28083.1516

چکیده

هدف: چالش اصلی ورزشکاران در اجرای تمرینات شدید، بروز فشار اکسایشی در بافت­های مختلف است. از این رو، هدف پژوهش حاضر بررسی اثر مصرف مکمل کورکومین در طول هشت هفته و 48 ساعت پایانی تمرین تناوبی شدید (HIIT) بر میزان فعالیت گلوتاتیون پراکسیداز (GPX) و مالون­دی­آلدئید (MDA) عضلة اسکلتی، قلب و کبد موش­های صحرایی نر ویستار بود. روش شناسی: در این پژوهش تجربی، تعداد 42 سر موش صحرایی نر ویستار با سن هشت هفته پس از یک هفته آشناسازی، به شش گروه (کنترل، کورکومین، 48 ساعت کورکومین، تناوبی، تناوبی+کورکومین و تناوبی+48ساعت کورکومین)، تقسیم شدند. تمرین تناوبی شدید شامل هشت هفته و پنج جلسه در هفته دویدن روی نوارگردان مخصوص جوندگان بود. مکمل کورکومین به­میزان 30 میلی­گرم به ازای هر کیلوگرم از وزن بدن به شکل داخل صفاقی (سه جلسه در هفته) یا (48 ساعت پایانی، هر 8 ساعت) تزریق شد. سطوح فعالیت GPX با روش الایزا و MDA با روش اسپکتروفتومتری اندازه­گیری شد. داده­ها با تحلیل واریانس یک­طرفه تحلیل شدند. یافته­ها: فعالیت GPX عضلة اسکلتی، قلب و کبد گروه تناوبی در مقایسه با گروه کنترل به­طور معنا­داری (05/0>P) کمتر و سطوح MDA در بافت قلب به‌طور معنا­داری (001/0=P) بالاتر بود. همچنین، میزان فعالیت GPX در عضلة اسکلتی (001/0=P)، قلب (004/0= P) و کبد (029/0= P) گروه تناوبی+کورکومین و همچنین قلب(008/0= P) تناوبی+48 ساعت کورکومین به‌طور معناداری از گروه تناوبی بیشتر بود. علاوه­براین، میزان سطوح MDA قلب در گروه­های تناوبی+کورکومین (001/0P =) و تناوبی+48 ساعت کورکومین (001/0P =) از گروه تناوبی کمتر بود. نتیجه­گیری: با وجود پاسخ­های اکسایشی متفاوت بافت­ها به تمرینات HIIT، مصرف مکمل کورکومین در 48 ساعت پایانی می­تواند همانند مصرف آن در طول هشت هفته از اثرات فشار اکسایشی جلوگیری کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Intake Duration of Curcumin Supplementation along with HIIT on Antioxidant Balance of Skeletal Muscle, Heart, and Liver in Male Rats

نویسندگان [English]

  • Ali Gorzi 1
  • Ahmad Rahmani 2
  • Samane Ekradi 3
1 Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran.
2 Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran.
3 Department of Exercise Physiology, University of Tarbiat Modarres, Tehran, Iran.
چکیده [English]

Aim:  Oxidative stress in different organs is the main challenge for athletes who undergo strenuous training. This study investigated the effect of curcumin supplementation along with HIIT on the antioxidant balance of skeletal muscle, heart, and liver in male Wistar rats. Methods: Forty two male Wistar rats aged six-eight weeks, after one week familiarization period, were randomly divided into 6 groups; Control, Curcumin, 48hrs Curcumin, HIIT, HIIT+Curcumin, and HIIT+48hrs Curcumin. HIIT training (8 weeks, 5 sessions a week) carried out on a rodent treadmill. Curcumin supplement (30 mg/kg.BW) injected Intraperitoneally (eight weeks, three times a week; or last 48 hours, every 8 hours) in the supplemented groups. GPX activity was measured by Elisa Kit and the MDA level was assessed by spectrophotometric method. Results: One-way ANOVA results showed that GPX activity of the HIIT group in skeletal muscle, heart, and liver were significantly (P<0.05) lower, and MDA levels of the heart were significantly (P=0.001) higher than the control group. Also, GPX activity of skeletal muscle (P=0.001), heart (P=0.004), and liver (P=0.029) in the HIIT+curcumin and HIIT+48hrs curcumin group of the heart (P=0.008) were significantly higher than HIIT group. MDA levels of heart in the HIIT+curcumin (p=0.001) and HIIT+48hrs curcumin (P=0.001) groups were significantly lower than the HIIT group. Conclusion: In spite of different oxidative stress responses of tissues to the HIIT training, curcumin supplementation during the last 48 hours of strenuous HIIT, as well as during eight weeks, could prevent oxidative stress, and improves antioxidant capacity.

کلیدواژه‌ها [English]

  • Curcumin
  • High Intensity Interval Training
  • Antioxidant Balance

Publisher: Azarbaijan Shahid Madani University          Copyright © The Authors   

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

مراجع

  1. Ascensão A, Ferreira R, Magalhães JJIjoc. Exercise-induced cardioprotection—biochemical, morphological and functional evidence in whole tissue and isolated mitochondria. 2007;117(1):16-30.
  2. Ji LLJPotSfeB, Medicine. Antioxidants and oxidative stress in exercise. 1999;222(3):283-92.
  3. Banerjee AK, Mandal A, Chanda D, Chakraborti SJM, biochemistry c. Oxidant, antioxidant and physical exercise. 2003;253(1):307-12.
  4. Gorzi A, Ekradi SJSP. The effect of intake duration of curcumin supplementation during strenuous endurance training on GPX activity and MDA levels of liver, heart and skeletal muscle in male Wistar rats. 2020;12(46):139-56.
  5. Filaire E, Rouveix M, Massart A, Gladine C, Davicco MJ, Durand D. Lipid peroxidation and antioxidant status in rat: effect of food restriction and wheel running. European journal of applied physiology. 2009;107(2):243-50.
  6. Antunes F, Han D, Cadenas E. Relative contributions of heart mitochondria glutathione peroxidase and catalase to H(2)O(2) detoxification in in vivo conditions. Free Radic Biol Med. 2002;33(9):1260-7.
  7. Atalay M, Lappalainen J, Sen CK. Dietary antioxidants for the athlete. Current sports medicine reports. 2006;5(4):182-6.
  8. Adams AK, Best TM. The role of antioxidants in exercise and disease prevention. The physician and sportsmedicine. 2002;30(5):37-44.
  9. Gharakhanlou R, Afzalpour ME, Gaeini AA, Rahnama N. Effects of aerobic exercises on the serum paraoxonase 1/arylesterase activity and lipid profile in non-active healthy men. International Journal of Sports Science and Engineering. 2007;1:105-12.
  10. de Zwart LL, Meerman JH, Commandeur JN, Vermeulen NP. Biomarkers of free radical damage: applications in experimental animals and in humans. Free Radical Biology and Medicine. 1999;26(1-2):202-26.
  11. Ericson-Neilsen W, Kaye ADJOJ. Steroids: pharmacology, complications, and practice delivery issues. 2014;14(2):203-7.
  12. Liu Z-C, Yang Z-X, Zhou J-S, Zhang H-T, Huang Q-K, Dang L-L, et al. Curcumin regulates hepatoma cell proliferation and apoptosis through the Notch signaling pathway. 2014;7(3):714.
  13. Prasad S, Tyagi AK, Aggarwal BBJCr, Association tojoKC. Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: the golden pigment from golden spice. 2014;46(1):2-18.
  14. Modasiya M, Patel VJIJPLS. Studies on solubility of curcumin. 2012;3(3):1490-7.
  15. Wei Y, Gong J, Yoshida T, Eberhart CG, Xu Z, Kombairaju P, et al. Nrf2 has a protective role against neuronal and capillary degeneration in retinal ischemia–reperfusion injury. 2011;51(1):216-24.
  16. Priyadarsini KI, Maity DK, Naik G, Kumar MS, Unnikrishnan M, Satav J, et al. Role of phenolic OH and methylene hydrogen on the free radical reactions and antioxidant activity of curcumin. 2003;35(5):475-84.
  17. Gupta SC, Patchva S, Koh W, Aggarwal BBJC, pharmacology e, physiology. Discovery of curcumin, a component of golden spice, and its miraculous biological activities. 2012;39(3):283-99.
  18. Suhett LG, de Miranda Monteiro Santos R, Silveira BKS, Leal ACG, de Brito ADM, de Novaes JF, et al. Effects of curcumin supplementation on sport and physical exercise: a systematic review. 2021;61(6):946-58.
  19. Gorzi A, Hosseini FJJoE, Talk OC. Muscle and serum antioxidant cross talk following curcumin and light resistance training during strenuous endurance training in male Wistar rats. 2021;1(2):86-92.
  20. Bańkowski S, Petr M, Rozpara M, Sadowska-Krępa EJRR. Effect of 6-week curcumin supplementation on aerobic capacity, antioxidant status and sirtuin 3 level in middle-aged amateur long-distance runners. 2022;27(1):186-92.
  21. Gorzi A, Kazemzadeh Y, Ahmadi PJSP. The effect of length of curcumin supplementation on antioxidant capacity of adolescent taekwondo players. 2016;8(29):131-44.
  22. Gorzi A, Rahmani A, Mohammadi Z, Neto WKJMBR. Effects of different lengths of high-intensity interval training microcycles on the systemic and hippocampal inflammatory state and antioxidant balance of immature rats. 2021:1-9.
  23. Gorzi A, Jamshidi F, Rahmani A, Neto WKJMBR. Muscle gene expression of CGRP-α, CGRP receptor, nAchR-β, and GDNF in response to different endurance training protocols of Wistar rats. 2020;47(7):5305-14.
  24. Gorzi A, Taherkhani L, Rahmani AJSJoKUoMS. Effect of folate supplementation during 10 weeks of HIIT on serum levels of ghrelin and leptin in male wistar rats. 2017;22(5):13-21.
  25. Gorzi A, Asadi M, Voltarelli F, Shamsi MMJCEP. Effects of curcumin on antioxidant capacity and gastric mucosal injury following strenuous endurance training in rats. 2021;17(1):17-24.
  26. Tesoriere L, D'Arpa D, Butera D, Pintaudi AM, Allegra M, Livrea MA. Exposure to malondialdehyde induces an early redox unbalance preceding membrane toxicity in human erythrocytes. Free radical research. 2002;36(1):89-97.
  27. Pinho CA, Tromm CB, Tavares AM, Silva LA, Silveira PCL, Souza CT, et al. Effects of different physical training protocols on ventricular oxidative stress parameters in infarction-induced rats. Life sciences. 2012;90(13-14):553-9.
  28. Azhdari A, Hosseini SA, Farsi S. Antioxidant effect of high intensity interval training on cadmium-induced cardiotoxicity in rats. Gene, Cell and Tissue. 2019;6(3).
  29. Fattahi Bafghi A, Homaee HM, Azarbayjani MA. Effects of high intensity interval training and curcumin supplement on antioxidant enzyme in heart tissue of diabetic rats. Iranian Journal of Diabetes and Obesity. 2016;8(3):135-41.
  30. Samadi A, Shemshaki A, Radaei. The Effect of Eight-Week Sprint Interval Training (SIT) on Oxidative/Antioxidant Status in Cardiac Tissue of Male Wistar Rats Under A High-Calorie High-Salt Diet. Jornal of Applied Health Studies in Sport Physiology. 2022;9(2):112-22. [in Persian].
  31. Songstad NT, Kaspersen K-HF, Hafstad AD, Basnet P, Ytrehus K, Acharya G. Effects of high intensity interval training on pregnant rats, and the placenta, heart and liver of their fetuses. PloS one. 2015;10(11):e0143095.
  32. Ascensão A, Ferreira R, Magalhães J. Exercise-induced cardioprotection—biochemical, morphological and functional evidence in whole tissue and isolated mitochondria. International journal of cardiology. 2007;117(1):16-30.
  33. Siu PM, Bryner RW, Martyn JK, Alway SE. Apoptotic adaptations from exercise training in skeletal and cardiac muscles. The FASEB Journal. 2004;18(10):1150-2.
  34. Emami A-M, Homaee HM, Azarbayjani MA. Effects of high intensity interval training and curcumin supplement on glutathione peroxidase (GPX) activity and malondialdehyde (MDA) concentration of the liver in STZ induced diabetic rats. Iranian Journal of Diabetes and Obesity. 2016;8(3):129-34.
  35. Gorzi A, Asadi MJZJoRiMS. Useful Effects of Curcumin Supplementation on Gastric Superoxide Dismutase Activity and Serum Malondialdehyde Level During Endurance Training in Male Wistar Rats. 2020;22(2).
  36. Kayatekin B, Gönenç S, Açikgöz O, Uysal N, Dayi A. Effects of sprint exercise on oxidative stress in skeletal muscle and liver. European journal of applied physiology. 2002;87(2):141-4.
  37. Groussard C, Maillard F, Vazeille E, Barnich N, Sirvent P, Otero YF, et al. Tissue-specific oxidative stress modulation by exercise: A comparison between MICT and HIIT in an obese rat model. Oxidative medicine and cellular longevity. 2019;2019.
  38. Pimenta M, Bringhenti I, Souza-Mello V, dos Santos Mendes IK, Aguila MB, Mandarim-de-Lacerda CA. High-intensity interval training beneficial effects on body mass, blood pressure, and oxidative stress in diet-induced obesity in ovariectomized mice. Life sciences. 2015;139:75-82.
  39. Cunningham P, Geary M, Harper R, Pendleton A, Stover S. high intensity sprint training reduces lipid peroxidation in fast-twitch skeletal muscle. Journal of Exercise Physiology Online. 2005;8(6).
  40. Radak Z, Chung HY, Goto S. Systemic adaptation to oxidative challenge induced by regular exercise. Free Radical Biology and Medicine. 2008;44(2):153-9.
  41. Afzalpour M, Gharakhanlou R, Gaeini A, MOHEBI H, Hedayati S. The effects of vigorous and moderate aerobic exercise on the serum arylesterase activity and total antioxidant capacity in non-active healthy men. 2006.
  42. Gorzi A, Asadi M. Useful Effects of Curcumin Supplementation on Gastric Superoxide Dismutase Activity and Serum Malondialdehyde Level During Endurance Training in Male Wistar Rats. Zahedan Journal of Research in Medical Sciences. 2020;22(2).
  43. Dabidi RV, Hosseinzadeh S, Mahjoub S, Hosseinzadeh M, Myers JJBos. Endurance exercise training and diferuloyl methane supplement: changes in neurotrophic factor and oxidative stress induced by lead in rat brain. 2013;30(1):41.
  44. Roshan VD, Assali M, Moghaddam AH, Hosseinzadeh M, Myers J. Exercise Training and Antioxidants Effects on Rat Heart Tissue Exposed to Lead Acetate. International journal of toxicology. 2011;30(2):190-6.
  45. Roshan VD, Rahimi M, Shirinbayan V, Mahjoub S, Hosseinzadeh M. Protective effect of the combination of exercise and curcumin supplementation on cardiac system in rats exposed to lead. International Journal of Nutrition and Metabolism. 2012;4(8):114-20.
  46. Gorzi A, Tofighi A, Amiri B. The effects of curcumin supplementation on oxidative stress induced during strenuous endurance training on the kidney and lung tissues. Sci J Kurdistan Univ Med Sci. 2018;23(5):1-11. (In Persian).
  47. Ms SAB, Waldman P, Hunter S, Krings P, Ben M, Lamberth P, John, Smith P, JohnEric W, McAllister P, Matthew J. Effect of curcumin supplementation on exercise-induced oxidative stress, inflammation, muscle damage, and muscle soreness. Journal of dietary supplements. 2020;17(4):401-14.
  48. Choromańska B, Myśliwiec P, Łuba M, Wojskowicz P, Myśliwiec H, Choromańska K, et al. Impact of weight loss on the total antioxidant/oxidant potential in patients with morbid obesity—a longitudinal study. 2020;9(5):376.
  49. Oguzturk H, Ciftci O, Aydin M, Timurkaan N, Beytur A, Yilmaz F. Ameliorative effects of curcumin against acute cadmium toxicity on male reproductive system in rats. Andrologia. 2012;44(4):243-9.
  50. Gorzi A, Asadi M. Useful Effects of Curcumin Supplementation on Gastric Superoxide Dismutase Activity and Serum Malondialdehyde Level During Endurance Training in Male Wistar Rats. Zahedan Journal of Research in Medical Sciences. 2020;22(2).
  51. Dabidi RV, Hosseinzadeh S, Mahjoub S, Hosseinzadeh M, Myers JJBos. Endurance exercise training and diferuloyl methane supplement: changes in neurotrophic factor and oxidative stress induced by lead in rat brain. 2013;30(1):41.
  52. Roshan VD, Assali M, Moghaddam AH, Hosseinzadeh M, Myers J. Exercise Training and Antioxidants Effects on Rat Heart Tissue Exposed to Lead Acetate. International journal of toxicology. 2011;30(2):190-6.
  53. Roshan VD, Rahimi M, Shirinbayan V, Mahjoub S, Hosseinzadeh M. Protective effect of the combination of exercise and curcumin supplementation on cardiac system in rats exposed to lead. International Journal of Nutrition and Metabolism. 2012;4(8):114-20.
  54. Gorzi A, Tofighi A, Amiri B. The effects of curcumin supplementation on oxidative stress induced during strenuous endurance training on the kidney and lung tissues. Sci J Kurdistan Univ Med Sci. 2018;23(5):1-11. (In Persian).
  55. Ms SAB, Waldman P, Hunter S, Krings P, Ben M, Lamberth P, John, Smith P, JohnEric W, McAllister P, Matthew J. Effect of curcumin supplementation on exercise-induced oxidative stress, inflammation, muscle damage, and muscle soreness. Journal of dietary supplements. 2020;17(4):401-14.
  56. Choromańska B, Myśliwiec P, Łuba M, Wojskowicz P, Myśliwiec H, Choromańska K, et al. Impact of weight loss on the total antioxidant/oxidant potential in patients with morbid obesity—a longitudinal study. 2020;9(5):376.
  57. Oguzturk H, Ciftci O, Aydin M, Timurkaan N, Beytur A, Yilmaz F. Ameliorative effects of curcumin against acute cadmium toxicity on male reproductive system in rats. Andrologia. 2012;44(4):243-9.
مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 10، شماره 1
فروردین 1402
صفحه 112-126

فایل ها

سابقه مقاله

  • تاریخ دریافت: 26 آبان 1401
  • تاریخ بازنگری: 28 بهمن 1401
  • تاریخ پذیرش: 30 بهمن 1401

هم رسانی

ارجاع به این مقاله

آمار