تاثیر سه شیوه تمرینات HIT ، HIIT و MIT بر بیان ژن لپتین و لیپوپروتئین لیپاز در بافت چربی زیر جلدی رت های نر

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 ارشد، گروه فیزیولوژی ورزش ، دانشگاه پیام نور، تهران صندوق پستی 3697-19395، ایران

2 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، صندوق پستی 3697-19395 تهران، ایران

3 استادیار،گروه تربیت بدنی ، بخش علوم تربیتی و روانشناسی ، دانشگاه پیام نور، تهران صندوق پستی 3697-19395، ایران.

10.22049/jahssp.2023.28026.1502

چکیده

هدف: لپتین و لیپوپروتئین لیپاز از فاکتورهای مهم در فرایند متابولیسم چربی و هموستاز انرژی می­باشد که اثر شیوه­های مختلف تمرین ورزشی، کمتر مورد مطالعه قرار گرفته است. هدف از پژوهش حاضر تاثیر  سه شیوه تمریناتHIT ،HIIT وMIT بر بیان ژن لپتین و  لیپوپروتئین لیپاز در بافت چربی زیر جلدی رت های نر بود. روش شناسی: تعداد ۳۲ سر رت نر با میانگین وزنی 25±237گرم تهیه شدند.موش ها به صورت تصادفی به چهار گروه مساوی کنترل ، تمرین هوازی با شدت متوسط، تمرین هوازی با شدت بالا و تمرین هوازی تناوبی با شدت بالا تقسیم شدند. گروه های تمرینی به مدت ۸ هفته در برنامه های تمرینی شرکت کردند. بیان ژنهای لپتین و لیپوپروتئین لیپاز با استفاده از روش آزمایشگاهی RT-PCR تعیین گردید.جهت بررسی تفاوت بین گروهی متغیرها از آزمون آماری تحلیل واریانس یکطرفه و آزمون تعقیبی توکی و از نرم افزار SPSS-24 استفاده شد. یافته­ها :نتایج نشان داد اختلاف معنی داری در بیان لپتین و لیپوپروتئین لیپاز بین گروه‌ها (کنترل،HIT ، HIIT و MIT) وجود داشت (001/0p=  ). نتایج آزمون توکی نشان داد اگرچه انجام تمریناتHIT ، HIIT و MIT باعث کاهش بیان ژن لپتین (001/0p=  ) و افزایش لیپوپروتئین لیپاز نسبت به گروه کنترل شد (001/0p=  ). با این حال تفاوت معناداری میان گروه­های تمرینی (HIT ، HIIT و MIT) در تغییرات لپتین و لیپوپروتئین لیپاز مشاهده نشد. نتیجه گیری :نتایج مطالعات حاضر نشان داد HIT ، HIIT و MIT هر سه سبب تغییرات بیان ژن لپتین و لیپوپروتئین لیپاز می­شود اما به نظر می­­رسد تغییرات لپتین و لیپوپروتئین لیپاز در تمرینات HIT بیشتر از MIT و HIIT بوده است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of three methods of HIT, HIIT and MIT training on leptin and lipoprotein lipase gene expression in subcutaneous fat tissue of male rats.

نویسندگان [English]

  • Shahrazad Ansari 1
  • Ali Barzegari 2
  • Saeed Naghibi 2
  • Mohammad Hassan Dashty 3
1 4. Master, Department of Exercise Physiology, Payame Noor University, Tehran PO Box 3697-19395, Iran
2 Assistant Professor, Department of physical education, Payame Noor University, PO BOX 19395-3697, Tehran, IRAN.
3 Assistant Professor, Department of Physical Education, Department of Educational Sciences and Psychology, Payame Noor University, Tehran PO Box 3697-19395, Iran.
چکیده [English]

    
Aim: Leptin and lipoprotein lipase are important factors in the process of fat metabolism and energy homeostasis, and the effect of different methods of exercise training has been less studied. The aim of this study was the effect of three types of HIT, HIIT and MIT exercises on leptin and lipoprotein lipase gene expression in the subcutaneous fat tissue of male rats. Methods: 32 male rats with an average weight of 237 ± 25 grams were prepared. Mice were randomly divided into four equal groups of control, moderate-intensity aerobic training, high-intensity aerobic training, and high-intensity intermittent aerobic training. The training groups participated in training programs for 8 weeks. The expression of leptin and lipoprotein lipase genes was determined using RT-PCR laboratory method. To investigate the difference between groups of variables, one-way analysis of variance and Tukey's post hoc test and SPSS-24 software were used.. Results:The results showed that there was a significant difference in the expression of leptin and lipoprotein lipase between the groups (control, HIT, HIIT and MIT)(p=0.001).Tukey's test results showed that although performingHIT, HIIT and MIT exercises decreased leptin gene expression (p=0.001) and increased lipoprotein lipase compared to the control group(p=0.001).However, no significant difference was observed between training groups(HIT, HIIT and MIT)in leptin and lipoprotein lipase changes.Conclusion:The results of the present study showed that HIT, HIIT and MIT all cause changAes in leptin and lipoprotein lipase gene expression, but it seems that the changes in leptin and lipoprotein lipase in HIT exercises were more than in MIT and HIIT.
 

کلیدواژه‌ها [English]

  • MIT
  • HIT
  • HIIT
  • leptin
  • lipoprotein lipase

Publisher: Azarbaijan Shahid Madani University          Copyright © The Authors   

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 10، شماره 1
فروردین 1402
صفحه 97-111

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  • تاریخ دریافت: 11 مهر 1401
  • تاریخ بازنگری: 01 بهمن 1401
  • تاریخ پذیرش: 02 بهمن 1401

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