تاثیر دو نوع تمرین تناوبی و استقامتی شدید به همراه مصرف مکمل عناب بر وضعیت استرس اکسایشی و ظرفیت‌های آنتی‌اکسیدانی بافت بیضه رت های نر ویستار نابالغ

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان، ایران

2 استادیار، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان ، ایران

10.22049/jahssp.2022.28004.1498

چکیده

هدف: مطالعه حاضر با هدف بررسی تاثیر تمرینات تناوبی و استقامتی شدید به همراه مصرف مکمل عناب  بر وضعیت استرس اکسایشی و ظرفیت­های آنتی‌اکسیدانی بافت بیضه موش‌های نابالغ انجام شد. روش شناسی: 72 سر موش نابالغ صحرایی نژاد ویستار با سن 22 روز و وزن 475/7 ± 64/60 گرم به ‌صورت تصادفی به 9 گروه :کنترل، تمرین تناوبی شدید (HIT)، تمرین استقامتی شدید (ICT)، عناب، تمرین استقامتی شدید + عناب، تمرین استقامتی شدید + دارونما، تمرین HIT + دارونما، تمرین HIT + عناب و دارونما تقسیم شدند. بعد از یک هفته آشناسازی، پروتکل ICT شامل دویدن بر روی نوار گردان به مدت 4 هفته (5 جلسه در هفته) و پروتکل HIT شامل 4 هفته (6 جلسه در هفته) بر روی نوار گردان جوندگان انجام شد. به گروه­های دارونما، گاواژ آب مقطر و به گروه‌های دارای عناب،گاواژ عصاره عناب با دوز 600 میلی­گرم به ازای هر کیلوگرم وزن بدن، روزانه بعد از تمرین ارائه شد. اندازه­گیری میزان فعالیت آنزیم‌های SOD ، GPX و CAT و سطوح MDA و TAC به روش اسپکتروفتومتری و کیت الایزا انجام شد. یافته‌ها: در گروه HIT افزایش معنی‌دار SOD، GPX و TAC پس از 4 هفته مشاهده شد (001/0=P)؛ در گروه ICT، میزان MDA پس از 4 هفته به‌طور معنی‌داری کاهش داشت (022/0=P)، در حالی که  SOD (006/0=P) و TAC (001/0=P) افزایش معنی­داری داشتند. میزان فعالیت آنتی‌اکسیدان ها و سطح  MDA و  TACدر گروه HIT + عصاره عناب تغییر معناداری نیافت، از طرفی در گروه ICT + عصاره عناب، تنها  CAT  کاهش معناداری داشت (012/0 =P). نتیجه‌گیری: نتایج تحقیق حاضر نشان داد تمرینات تناوبی و استقامتی شدید موجب کاهش فشار اکسایشی و افزایش معنی‌دار آنزیم‌های آنتی‌اکسیدانی در بافت بیضه و موش‌های صحرایی نابالغ شده است. با این‌ وجود با توجه به مطالعات اندک صورت گرفته در این زمینه، نیاز به تحقیقات بیشتری می‌باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of High Intensity Interval Training and Endurance Training Along With Jujube Supplement Consumption on the State of Oxidative Stress and Antioxidant Capacities of Testicular Tissue of Immature Male Wistar Rats

نویسندگان [English]

  • Akram Karimiasl 1
  • Farideh Ghasemikalateh 1
  • Ahmad Rahmani 1
  • Hamid Reza Norouzi 2
1 Department of Sport sciences, faculty of humanity, university of Znjan, Zanjan, Iran
2 Assistant Professor,Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran
چکیده [English]

Aim: The aim of this study was to investigating the effect of high intensity interval training and endurance training along with jujube supplement consumption on the state of oxidative stress and antioxidant capacity of testicular tissue of immature rats. Methods: 72 immature wistar rats with age of 22 days and weight of 60.64 ± 7.475 grams were randomly divided into 9 groups: control, intense interval training (HIT), intense endurance training (ICT), jujube , ICT + jujube, ICT + placebo, HIT training + placebo, HIT training + jujube and placebo. After a week of familiarization, the ICT protocol including running on a  rodent treadmill for 4 weeks (5 sessions/week) and the HIT protocol including 4 weeks (6 sessions/week) were performed. Distilled water gavage was given to the placebo groups, and jujube extract gavage was given to the jujube groups at a dose of 600 mg/kg of body weight, daily after training. The activities of SOD, GPX and CAT enzymes and MDA and TAC levels were measured by spectrophotometry and ELISA kit. Results: In the HIT group, a significant increase in SOD, GPX and TAC was observed after 4 weeks (P=0.001); In the ICT group, the amount of MDA decreased significantly after 4 weeks (P=0.022), while SOD (P=0.006) and TAC (P=0.001) had a significant increase. The amount of antioxidant activity and the level of MDA and TAC did not change significantly in the group of HIT + jujube extract, on the other hand, in the ICT + jujube extract group, only CAT had a significant decrease (P = 0.012). Conclusions:  The results of the present study showed that intense interval and endurance training has reduced oxidative stress and significantly increased antioxidant enzymes in testicular tissue and immature rats. However, due to the few studies done in this field, more research is needed.

کلیدواژه‌ها [English]

  • Malondialdehyde (MDA)
  • Superoxide dismutase (SOD)
  • Glutathione peroxidase (GPX)
  • Catalase (CAT)
  • Total antioxidant capacity (TAC)

Publisher: Azarbaijan Shahid Madani University          Copyright © The Authors   

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 10، شماره 1
فروردین 1402
صفحه 67-82

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  • تاریخ دریافت: 23 شهریور 1401
  • تاریخ بازنگری: 07 دی 1401
  • تاریخ پذیرش: 07 دی 1401

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