تأثیر تمرین مقاومتی همراه با مصرف مکمل کورکومین بر بیان برخی ژن‌های تنظیم گر مرتبط با ساختار عضله قلبی رت‌های چاق

نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I


1 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد لارستان، لارستان، ایران

2 دانشیار فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد مرودشت، مرودشت، ایران



هدف: پیری قلب نتیجه‌ی اصلی چاقی است که به دنبال آن هایپرتروفی پاتولوژیک قلبی به وجود می‌آید. اما تمرین مقاومتی و پلی فنول‌های موجود در کورکومین در تعدیل برخی مسیرهای پیام‌رسان درون سلولی نقش دارند. بنابراین هدف از پژوهش حاضر، بررسی اثر تمرینات مقاومتی و مکمل کورکومین بر بیان ژن‌های تنظیم‌گر مرتبط با ساختار عضله قلبی رت‌ها بود. روش‌شناسی: در این پژوهش تجربی18 سر رت نر نژاد اسپراگ داولی بعد از هشت هفته استفاده از رژیم غذایی پرچرب به سه گروه شامل کنترل غیرچاق (6=n)، چاق مرجع (6=n) و تمرین +کورکومین(6=n) تقسیم شدند. تمرین مقاومتی به مدت هشت هفته، سه جلسه در هفته (بر علیه 20 تا 50 درصد از وزن بدن) اجرا شد. همزمان روزانه کورکومین خالص با دوز 150 میلی‌گرم به ازای هر کیلوگرم وزن بدن به صورت گاواژ مصرف شد. مقدار بیان ژن‌های (AMPK ،mTOR ، S6K ، 4EBP، COL1، COL3، Ang II) با تکنیک Real time-PCR تعیین شد و داده‌ها با استفاده از آزمون آماری تحلیل واریانس یکطرفه تحلیل شدند. یافته‌ها: چاقی باعث کاهش بیان ژن‌ AMPK و افزایش بیان ژنهای mTOR، S6K، 4EBPT، COL1، COL3، Ang II شد، در حالی که در گروه تمرین+کورکومین در مورد همه این متغیرها، تفاوت معنی‌داری نسبت به گروه چاق مرجع مشاهده شد( 0/05>P). نتیجه‌گیری: به نظر می‌رسد چاقی باعث سرعت گرفتن فرآیندهای منجر شونده به هایپرتروفی پاتولوژیک قلبی می‌شود، اما تمرین مقاومتی و مکمل کورکومین تا حدودی این اثرات منفی را کاهش می‌دهند.


عنوان مقاله [English]

Effect of Resistance Training Combined with Curcumin Supplementation on Expression of Regulatory Genes Related to Myocardial Remodeling in Obese Rats

نویسندگان [English]

  • Aida Moieni 1
  • Seyed Ali Hosseini 2
1 Ph.D Student in Exercises Physiology, Department of Physical Education and Sport Sciences, Sport Physiology Department, Islamic Azad University, Larestan Branch, Larestan, Iran
2 Associate Professor in exercises Physiology, Department of exercises Physiology, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran
چکیده [English]

Aim: Cardiac aging is a major consequence of obesity that results in pathologic cardiac hypertrophy. However, resistance training and curcumin polyphenols can modulate some of involved intracellular signaling pathways. Therefore, the aim of this study was to investigate the effect of resistance training and curcumin supplementation on expression of regulatory genes involved in cardiac structural remodeling. Methods: in this experimental study, 18 male Sprague Dawley rats were divided into Normal Weight Control (n=6), Obese Reference (n = 6), Training + Curcumin (n = 6) groups following to eight weeks of high fat diet consumption. Resistance training were conducted also for eight weeks, three sessions per week against 20 to 50% of body weight. Curcumin (150 mg per kg body weight, daily) was consumed simultaneously through gavage. The expression levels of AMPK, mTOR, S6K, 4EBP, COL1, COL3 and AngII genes were assessed using Real Time-PCR technique and thy data was analyzed by One-way ANOVA. Results: Obesity was lead to AMPK gene expression level downregulation, while expression levels of mTOR, S6K, 4EBP, COL1, COL3, AngII genes were up-regulated (p < 0.05). However; there were also significant differences in expression levels of these genes between Training + Curcumin and Obese Reference groups (p < 0.05). Conclusion: It seems obesity causes acceleration of the processes involved in cardiac pathologic hypertrophy, while resistance training with curcumin supplementation could decrease this hazards to some extent.

کلیدواژه‌ها [English]

  • Obesity
  • curcumin
  • Myocardium
  • resistance training
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