تأثیر مکمل کافئین بر فعالیت میلوپراکسیداز و استیل کولین استراز در طی فعالیت ورزشی مقاومتی حاد در ورزشکاران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشگاه کردستان، سنندج، ایران

2 دانشجوی کارشناس ارشد، گروه فیزیولوژی ورزشی، دانشگاه کردستان، سنندج، ایران.

10.22049/jassp.2019.26533.1207

چکیده

کافئین بر پاسخ ایمنی بـدن حین فعالیت ورزشی اثرگـذار اسـت و نشان داده‌ شده که کافئین غلظت استیل‌کولین‌استراز و میلوپراکسیداز در موش‌های تمرین کرده را کاهش داده است؛ بنابراین در این پژوهش اثر مصرف کافئین بر فعالیت آنزیم‌های استیل کولین‌استراز و میلوپراکسیداز پس از فعالیت ورزشی مقاومتی در ورزشکاران مورد بررسی قرار گرفت. پانزده ورزشکار در مطالعه تصادفی، دوسوکور، کنترل‌شده با دارونما و متقاطع، 6 میلی گرم کافئین و دارونما (مالتودکسترین) به ازای هر کیلوگرم وزن بدن را یک ساعت قبل از فعالیت مقاومتی دریافت کردند. نمونه‌گیری خون از سیاهرگ بازویی آزمودنی‌ها قبل، بعد و یک ساعت بعد از فعالیت ورزشی مقاومتی جهت اندازه‌گیری غلظت میلوپراکسیداز و استیل‌کولین‌استراز گرفته شد. آزمون‌ تحلیل واریانس با اندازه‌گیری مکرر در سطح معناداری 05/0 استفاده گردید. نتایج تحلیل واریانس با اندازه‌گیری مکرر نشان داد که در غلظت استیل‌کولین‌استراز تفاوت معنی‌داری در بلافاصله و 15 دقیقه بعد از فعالیت مقاومتی بین کافئین و دارونما وجود دارد (05/0>P، اما در ارتباط با غلظت میلوپراکسیداز تفاوت معنی دار بین شرایط کافئین و دارونما وجود نداشت (05/0<P). در شرایط مصرف کافئین غلظت استیل‌کولین‌استراز در پس‌آزمون به‌طور معنی‌داری پایین‌تر از مقادیر پیش‌آزمون بود، اما در شرایط مصرف دارونما غلظت استیل کولین استراز در پس‌آزمون افزایش نسبت به پیش‌آزمون مشاهده شد (05/0>P). به نظر می‌رسد که مصرف مکمل کافئین قبل از فعالیت مقاومتی از افزایش غلظت آنزیم استیل‌کولین‌استراز در مردان ورزشکار جلوگیری می‌کند و احتمالاً کافئین از این طریق سبب کاهش فعالیت‌های التهابی می‌گردد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Caffeine Supplement on Myeloperoxidase and Acetylcholinesterase Activity during Acute Resistance Exercise in Athletes

نویسندگان [English]

  • Mohammad Rahman Rahimi 1
  • Chia Naghshini 2
1 Associate Professor of Exercise Physiology, Department of Exercise Physiology, University of Kurdistan, Sanandaj, Iran.
2 MSc. in Exercise Physiology, Department of Exercise Physiology, University of Kurdistan, Sanandaj, Iran.
چکیده [English]

Caffeine affects immune response during exercise and it has shown that caffeine reduced concentration of acetyl cholinesterase and myeloperoxidase in mice trained. Thus, in this study the effect of caffeine ingestion on enzyme activities of acetyl cholinesterase and myeloperoxidase was examined after resistance exercise in athletes.
15 athletes in in a randomized, double-blind, placebo-controlled, cross-over study, received 6 mg.kg-1 of caffeine and placebo (maltodextrin) one hour before the resistance exercise (RE). After an hour blood samples were taken from the participants then they participated in resistance exercise and immediately and 15 minutes after resistance exercise, another’s blood samples were taken to analysis acetyl cholinesterase and myeloperoxidase concentration. Analysis of variance with repeated measures at α level 0.05.was used.
ANOVA with repeated measures showed significant difference in concentration of acetyl cholinesterase at immediately and 15 minutes after resistance exercise between caffeine and placebo condition (p<0.05), but there was no significant difference between caffeine and placebo in relation to the concentration of myeloperoxidase (p>0.05). In terms of caffeine consumption, acetyl cholinesterase concentration was significantly lower at post compared to pre RE, but in terms of placebo consumption, acetyl cholinesterase concentration was significantly higher at post compared to pre RE (p<0.05).
It seems that consumption of caffeine supplement before resistance exercise prevent increase in concentration of acetyl cholinesterase in male athletes, and possibly caffeine from this way lead to reduce inflammatory activities.

کلیدواژه‌ها [English]

  • Caffeine
  • myeloperoxidase
  • acetylcholinesterase
  • Resistance Exercise

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