نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I
نویسندگان
1 دانشیار گروه فیزیولوژی ورزشی، دانشگاه کردستان، سنندج، ایران
2 استاد فیزیولوژی، گروه علوم زیستی, دانشکده علوم پایه، دانشگاه کردستان، سنددج، ایران.
3 استادیار فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، واحد مریوان، دانشگاه آزاد اسلامی، مریوان، ایران.
4 دانشجوی دکتری فیزیولوژی ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه کردستان، کردستان
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background& purpose: Endurance exercise and caffeine consumption modulate cardiac cell signaling pathways; however, the interactive effects of these interventions on the expression of apoptosis-regulating proteins in myocardial tissue have not yet been fully elucidated. The present study aimed to investigate the effects of eight weeks of endurance training and caffeine supplementation on cardiac apoptosis factors (BAX, BCL2, P53, Caspase3, and miR-499) in male Wistar rats. Methods:Thirty-two rats were divided into four groups: control (ND), caffeine (ND+CAF), endurance training (ND+ET), and combined training + caffeine (ET+CAF). The caffeine groups received 6 mg/kg/day of caffeine via oral gavage. Endurance training was performed on a treadmill for eight weeks (five sessions per week), with intensity increasing from 12 m/min (15 min) in the first week to 27 m/min (60 min) by the eighth week. The relevant factors were measured using Western blot and Real time PCR methods. One Way-ANOVA test was used and Tukey's post hoc test was used to compare the two groups. Results: The results showed that the level of proapoptotic protein BAX was higher in the caffeine group, while it was lower in the endurance training group and the combination of training and caffeine group (P=0.001). Also, the level of antiapoptotic protein BCL2 was higher in the combination of training and caffeine group (P=0.001). The expression level of miR-499 in the endurance training group and the combination of training and caffeine group was higher than that in the control group (P=0.001), and caspase 3 was lower in all groups than in the control group (P=0.001), but it had no significant effect on P53 (P=0.873). Conclusion: endurance training effectively mitigates cardiac apoptosis, and when combined with caffeine supplementation, it enhances anti-apoptotic signaling—indicating a synergistic cardioprotective effect mediated by BCL-2 upregulation, miR-499 activation, and suppression of caspase-dependent apoptotic pathways.
کلیدواژهها [English]
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