تاثیر تمرین تناوبی با شدت بالا بر بیان FGF21 عضله نعلی و پروفایل لیپیدی در موش‌های صحرایی تغذیه شده با رژیم پرچرب

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، دانشگاه تربیت مدرس، تهران، ایران.

2 گروه تربیت بدنی و علوم ورزشی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

هدف: راهکارهای دقیق درمانی و همزمان بررسی مکانیسم‌های درگیر در چاقی بسیار ضروری است. در نتیجه هدف از انجام تحقیق حاضر تاثیر تمرین تناوبی با شدت بالا بر بیان FGF21 عضله نعلی و پروفایل لیپیدی در موش‌های صحرایی تغذیه شده با رژیم پرچرب بود. روش پژوهش: بدین منظور 24 سر رت نر (سن: شش هفته، وزن: 20±200 گرم) به طور تصادفی به چهار گروه کنترل (CON) (6N=)، کنترل+تمرین (CON+EXE) (6N=)، رژیم پرچرب (HFD) (6N=) و رژیم پرچرب+تمرین (HFD+EXE) (6N=) تقسیم شدند. مداخله رژیم غذایی و ورزش همزمان شروع شد و به مدت 10 هفته ادامه داشت. تمرین HIIT هشت دوره فعالیت اینتروال (90 درصدMRC   به مدت 5/2 دقیقه) با دوره­های استراحت فعال (50 درصد MRC) به مدت 5/2 دقیقه) بود. برای بررسی FGF21 عضله نعلی از روش  PCR_qRT  استفاده شد. همچنین شاخص­های لیپیدی در سرم نیز اندازه‌گیری شد. برای تحلیل داده­ها از آزمون آنوای یکطرفه و آزمون تعقیبی توکی استفاده شد. سطح معنی­داری 05/0>P در نظر گرفته و آنالیزهای آماری توسط نرم­افزار SPSS27 مورد ارزیابی قرار گرفت. یافته‌ها: با انجام HIIT، کاهش وزن و BMI و هم­چنین کاهش LDL، کلسترول و افزایش HDL و نسبت HDL/LDL در گروه­های تمرین در مقایسه با کنترل­ مشاهده شد (001/0P=). بیان FGF21 عضله نعلی در گروه­های تمرین در مقایسه با گروه­های کنترل خود افزایش معنی­دار داشتند (001/0P=). نتیجه‌گیری: ده هفته انجام HIIT بدون محدودیت کالریک؛ می­تواند به طور بالقوه موجب افزایش بیان FGF21 و اثرات مثبت آن بر بهبود پروفایل لیپیدی و کاهش وزن شود. پس این نوع تمرین می‌تواند چاقی و عوارض ناشی از آن بدون محدودیت کالریک نیز تعدیل و تنظیم کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of High-Intensity Interval Training on Soleus Muscle FGF21 Expression and Lipid Profile in Rats Fed a High-Fat Diet

نویسندگان [English]

  • Mozhgan Rezvani Rad 1
  • Mahdieh Molanouri Shamsi 1
  • Reza Gharakhanlou 2
  • Shahnaz Shahrbanian 2
1 Department of Physical Education and Sport Sciences, Tarbiat Modares University, Tehran, Iran.
2 Department of Physical Education and Sport Sciences, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Aim:       Precise therapeutic strategies and simultaneous investigation of the mechanisms involved in obesity are essential. Therefore, the present study aimed to investigate the effect of high-intensity interval training on soleus muscle FGF21 expression and lipid profile in rats fed a high-fat diet. Methods: For this purpose, 24 healthy adult male rats (Age: six weeks, weight: 200±20 grams) were randomly divided into four groups: control (CON) (N=6), control+exercise (CON+EXE) (N=6), high-fat diet (HFD) (N=6), and high-fat diet+exercise (HFD+EXE) (N=6). Diet and exercise interventions were initiated simultaneously and continued for 10 weeks. HIIT training consisted of eight periods of interval activity (90% MRC for 2.5 min) with active rest periods (50% MRC) for 2.5 min). The PCR_ method was used to examine FGF21 expression in the soleus muscle. Serum lipid indices were also measured.  One-way ANOVA and Tukey's post hoc test were used to analyze the data. The significance level was considered to be P<0.05, and statistical analyses were evaluated using SPSS27 software. Results: By performing HIIT, weight and BMI were reduced, and LDL, cholesterol, HDL, and HDL/LDL ratio were increased in the training groups compared to those in the control group (P=0.001). The expression of FGF21 in the soleus muscle was significantly higher in the training group than in the control group (P=0.001). Conclusions:  Ten weeks of HIIT without caloric restriction could potentially increase FGF21 expression and improve lipid profiles and weight loss. Therefore, this type of training can modulate and regulate obesity and its complications, without caloric restriction.

کلیدواژه‌ها [English]

  • Obesity
  • High-Fat Diet
  • Skeletal Muscle
  • Fibroblast Growth Factor 21 (FGF21)
  • High-Intensity Interval Training (HIIT)

مراجع

  1. Vecchiatto B, de Castro TL, Muller CR, Azevedo-Martins AK, Evangelista FS. Physical exercise-induced FGF-21 to fight obesity: An update review. Obesities. 2022;2(4):372-9.
  2. Liu C, Yan X, Zong Y, He Y, Yang G, Xiao Y, et al. The effects of exercise on FGF21 in adults: a systematic review and meta-analysis. PeerJ. 2024;12:e17615.
  3. Li X-H, Liu L-Z, Chen L, Pan Q-N, Ouyang Z-Y, Fan D-J, et al. Aerobic exercise regulates FGF21 and NLRP3 inflammasome-mediated pyroptosis and inhibits atherosclerosis in mice. PLOS ONE. 2022;17(8):e0273527.
  4. Wang Z, Sun T, Yu J, Li S, Gong L, Zhang Y. FGF21: A Sharp Weapon in the Process of Exercise to Improve NAFLD. Frontiers in Bioscience-Landmark. 2023;28(12):351.
  5. Tabari FS, Karimian A, Parsian H, Rameshknia V, Mahmoodpour A, Majidinia M, et al. The roles of FGF21 in atherosclerosis pathogenesis. Reviews in Endocrine and Metabolic Disorders. 2019;20:103-14. [In Persian]
  6. Hughey CC, Bracy DP, Rome FI, Goelzer M, Donahue EP, Viollet B, et al. Exercise training adaptations in liver glycogen and glycerolipids require hepatic AMP-activated protein kinase in mice. American Journal of Physiology-Endocrinology and Metabolism. 2024;326(1):E14-E28.
  7. Richter MM, Kemp IM, Heebøll S, Winther-Sørensen M, Kjeldsen SA, Jensen NJ, et al. Glucagon augments the secretion of FGF21 and GDF15 in MASLD by indirect mechanisms. Metabolism. 2024;156:155915.
  8. Yano K, Yamaguchi K, Seko Y, Okishio S, Ishiba H, Tochiki N, et al. Hepatocyte-specific fibroblast growth factor 21 overexpression ameliorates high-fat diet-induced obesity and liver steatosis in mice. Laboratory Investigation. 2022;102(3):281-9.
  9. Le TD, Fathi P, Watters AB, Ellis BJ, Besing G-LK, Bozadjieva-Kramer N, et al. Fibroblast growth factor-21 is required for weight loss induced by the glucagon-like peptide-1 receptor agonist liraglutide in male mice fed high carbohydrate diets. Molecular Metabolism. 2023;72:101718.
  10. Solomon TP, Carter S, Haus JM, Karstoft K, von Holstein-Rathlou S, Nielsen MS, et al. Plasma FGF21 concentrations are regulated by glucose independently of insulin and GLP-1 in lean, healthy humans. PeerJ. 2022;10:e12755.
  11. Khalafi M, Alamdari KA, Symonds ME, Nobari H, Carlos-Vivas J. Impact of acute exercise on immediate and following early post-exercise FGF-21 concentration in adults: Systematic review and meta-analysis. Hormones. 2021;20:23-33. [In Persian]
  12. Kim H, Jung J, Park S, Joo Y, Lee S, Sim J, et al. Exercise-induced fibroblast growth factor-21: A systematic review and meta-analysis. International Journal of Molecular Sciences. 2023;24(8):7284.
  13. Porflitt-Rodríguez M, Guzmán-Arriagada V, Sandoval-Valderrama R, Tam CS, Pavicic F, Ehrenfeld P, et al. Effects of aerobic exercise on fibroblast growth factor 21 in overweight and obesity. A systematic review. Metabolism. 2022;129:155137.
  14. Tanimura Y, Aoi W, Takanami Y, Kawai Y, Mizushima K, Naito Y, et al. Acute exercise increases fibroblast growth factor 21 in metabolic organs and circulation. Physiological reports. 2016;4(12):e12828.
  15. Tofighi A, Alizadeh R, Tolouei Azar J. THE EFFECT OF EIGHT WEEKS HIGH INTENSITY INTERVAL TRAINING (HIIT) ON SERUM AMOUNTS OF FGF21 AND IRISIN IN SEDENTARY OBESE WOMEN. Studies in Medical Sciences. 2017;28(7):453-66. [In Persian]
  16. Slusher A, Whitehurst M, Zoeller R, Mock J, Maharaj M, Huang C-J. Attenuated fibroblast growth factor 21 response to acute aerobic exercise in obese individuals. Nutrition, Metabolism and Cardiovascular Diseases. 2015;25(9):839-45.
  17. Kong Z, Sun S, Liu M, Shi Q. Short‐term high‐intensity interval training on body composition and blood glucose in overweight and obese young women. Journal of diabetes research. 2016;2016(1):4073618.
  18. Cheragh Birjandi S, Saghebjoo M, Hedayati M. Effect of high intensity interval training and L-Arginine supplementation on serum levels of fibroblast growth factor 21 and atrial natriuretic peptide in overweight and obese young men. Journal of Birjand University of Medical Sciences. 2016;23(3):211-21. [In Persian]
  19. Li X-H, Liu L-Z, Chen L, Pan Q-N, Ouyang Z-Y, Fan D-J, et al. Aerobic exercise regulates FGF21 and NLRP3 inflammasome-mediated pyroptosis and inhibits atherosclerosis in mice. PloS one. 2022;17(8):e0273527.
  20. Martinez-Huenchullan SF, Ban LA, Olaya-Agudo LF, Maharjan BR, Williams PF, Tam CS, et al. Constant-moderate and high-intensity interval training have differential benefits on insulin sensitive tissues in high-fat fed mice. Frontiers in physiology. 2019;10:459.
  21. Hariri N, Thibault L. High-fat diet-induced obesity in animal models. Nutrition Research Reviews. 2010;23(2):270-99. [In Persian]
  22. Lee MO. DETERMINATION OF THE SURFACE AREA OF THE WHITE RAT WITH ITS APPLICATION TO THE EXPRESSION OF METABOLIC RESULTS. American Journal of Physiology-Legacy Content. 1929;89(1):24-33.
  23. Khalafi M, Mohebbi H, Karimi P, Faridnia M, Tabari E. The Effect of High Intensity Interval training and Moderate Intensity Continuous Training on Mitochondrial Content and PGC-1α of Subcutaneous Adipose Tissue in Male Rats with High Fat Diet Induced Obesity. Journal of Sport Biosciences. 2018;10(3):297-315. [In Persian]
  24. Öner S, Yasul Y, Akçinar F. The effects of high-intensity interval training on body composition and lipid profile. Pakistan Journal of Medical and Health Sciences. 2021;15(2):641-5.
  25.  [In Persian]Ghasemi, Faezeh, & Mohebbi, Hamid. Twelve weeks of high-intensity interval training, independent of hypoxia, induce hepatic mitochondrial biogenesis in male rats fed a high-fat diet. Metabolism and Exercise, 2024; 14(2): 189-208. doi: 10.22124/jme.2023.26237.339
  26. Racil G, Chelly M-S, Coquart J, Padulo J, Teodor DF, Russo L. Long-and Short-Term High-Intensity Interval Training on Lipid Profile and Cardiovascular Disorders in Obese Male Adolescents. Children. 2023;10(7):1180.
  27. Molina C, Cifuentes G, Martinez C, Mancilla R, Diaz E. Effects of 12 sessions of high intensity intermittent training and nutrition counseling on body fat in obese and overweight participants. Revista médica de Chile. 2016;144(10):1254-9.
  28. Keating SE, Johnson NA, Mielke GI, Coombes JS. A systematic review and meta‐analysis of interval training versus moderate‐intensity continuous training on body adiposity. Obesity reviews. 2017;18(8):943-64.
  29. Astorino TA, Heath B, Bandong J, Ordille GM, Contreras R, Montell M, et al. Effect of periodized high intensity interval training (HIIT) on body composition and attitudes towards hunger in active men and women. The Journal of sports medicine and physical fitness. 2017;58(7-8):1052-62.
  30. Alkahtani SA, Byrne NM, Hills AP, King NA. Interval training intensity affects energy intake compensation in obese men. International journal of sport nutrition and exercise metabolism. 2014;24(6):595-604.
  31. Sawyer BJ, Tucker WJ, Bhammar DM, Ryder JR, Sweazea KL, Gaesser GA. Effects of high-intensity interval training and moderate-intensity continuous training on endothelial function and cardiometabolic risk markers in obese adults. Journal of Applied Physiology. 2016;121(1):279-88.
  32. Ouerghi N, Ben Fradj MK, Khammassi M, Feki M, Kaabachi N, Bouassida A. Plasma chemerin in young untrained men: association with cardio-metabolic traits and physical performance, and response to intensive interval training. Neuroendocrinology letters. 2017;38(1):59-66.
  33. Fisher G, Brown AW, Bohan Brown MM, Alcorn A, Noles C, Winwood L, et al. High intensity interval-vs moderate intensity-training for improving cardiometabolic health in overweight or obese males: a randomized controlled trial. PloS one. 2015;10(10):e0138853.
  34. Pedersen TR, Olsson AG, Færgeman O, Kjekshus J, Wedel H, Berg Kr, et al. Lipoprotein changes and reduction in the incidence of major coronary heart disease events in the Scandinavian Simvastatin Survival Study (4S). Circulation. 1998;97(15):1453-60.
  35. Spaulding HR, Yan Z. AMPK and the adaptation to exercise. Annual review of physiology. 2022;84(1):209-27.
  36. Li H, Dun Y, Zhang W, You B, Liu Y, Fu S, et al. Exercise improves lipid droplet metabolism disorder through activation of AMPK-mediated lipophagy in NAFLD. Life sciences. 2021;273:119314.
  37. Kachur S, Lavie CJ, De Schutter A, Milani RV, Ventura HO. Obesity and cardiovascular diseases. Minerva medica. 2017;108(3):212-28.
  38. Poznyak AV, Melnichenko AA, Wetzker R, Gerasimova EV, Orekhov AN. NLPR3 inflammasomes and their significance for atherosclerosis. Biomedicines. 2020;8(7):205.
  39. Hong J, Park E, Lee J, Lee Y, Rooney BV, Park Y. Exercise training mitigates ER stress and UCP2 deficiency-associated coronary vascular dysfunction in atherosclerosis. Scientific reports. 2021;11(1):15449.
  40. Lee J, Lee Y, LaVoy EC, Umetani M, Hong J, Park Y. Physical activity protects NLRP 3 inflammasome‐associated coronary vascular dysfunction in obese mice. Physiological reports. 2018;6(12):e13738.
  41. Domouzoglou EM, Naka KK, Vlahos AP, Papafaklis MI, Michalis LK, Tsatsoulis A, et al. Fibroblast growth factors in cardiovascular disease: The emerging role of FGF21. American Journal of Physiology-Heart and Circulatory Physiology. 2015;309(6):H1029-H38.
  42. Fisher FM, Maratos-Flier E. Understanding the physiology of FGF21. Annual review of physiology. 2016;78:223-41.
  43. Mounesan A, Nourzad F. The effect of aerobic and resistance exercise on sugar indicators and FGF21 in cardiac tissue of diabetic rat. Journal of Exercise & Organ Cross Talk. 2023;3(4):208-15.
  44. Yang SJ, Hong HC, Choi HY, Yoo HJ, Cho GJ, Hwang TG, et al. Effects of a three‐month combined exercise programme on fibroblast growth factor 21 and fetuin‐A levels and arterial stiffness in obese women. Clinical endocrinology. 2011;75(4):464-9.
  45. Nawrocki AR, Rajala MW, Tomas E, Pajvani UB, Saha AK, Trumbauer ME, et al. Mice lacking adiponectin show decreased hepatic insulin sensitivity and reduced responsiveness to peroxisome proliferator-activated receptor γ agonists. Journal of Biological Chemistry. 2006;281(5):2654-60.
مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 13، شماره 1
فروردین 1405
صفحه 118-130

فایل ها

سابقه مقاله

  • تاریخ دریافت: 15 دی 1403
  • تاریخ بازنگری: 25 فروردین 1404
  • تاریخ پذیرش: 26 فروردین 1404

هم رسانی

ارجاع به این مقاله

آمار