تأثیر تمرینات تناوبی با شدت بالا و تداومی با شدت متوسط بر محتوای پروتئینی PI3K، AKT و FOXO3 قلب موش‌های تغذیه شده با رژیم غذایی پرچرب

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشیار، گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روان شناسی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 پژوهشگر پسادکتری، گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روان شناسی، دانشگاه محقق اردبیلی، اردبیل، ایران

3 دانشیار گروه تربیت بدنی و علوم ورزشی، واحد گچساران، دانشگاه آزاد اسلامی، گچساران، ایران

چکیده

هدف: اگرچه عدم فعالیت ورزشی و رژیم غذایی پرچرب (HFD) باعث بر هم زدن تعادل مصرف انرژی و دریافت انرژی می­شود اما، تمرین ورزشی از چاقی پیشگیری و آن را کنترل می­کند. بنابراین، این مطالعه با هدف بررسی تأثیر تمرین تناوبی با شدت بالا (HIIT) و تمرین تداومی با شدت متوسط ​​(MICT) بر محتوای پروتئینی PI3K، AKT و FOXO3 قلب موش‌های تغذیه‌شده با HFD انجام شد. روش پژوهش: در مطالعه تجربی حاضر، 20 سر موش صحرایی نر نژاد ویستار (سن حدود پنج هفته و میانگین وزن 10±110 گرم) خریداری و به­طور تصادفی در چهار گروه پنج‌تایی تقسیم شدند: کنترل سالم با رژیم غذایی طبیعی (HC)، چاق غیرفعال (SC)، HIIT به همراهHFD  (HIIT) و MICT به همراه HFD (MICT). برای القای چاقی، حیوانات هشت هفته رژیم غذایی پرچرب داشتند. هشت هفته HIIT و MICT برای هر گروه به­صورت مجزا انجام شد. برای بررسی بیان پروتئین­های PI3K، AKT و FOXO3از روش وسترن بلات و برای تجزیه‌وتحلیل داده­ها از تحلیل واریانس یک­طرفه با آزمون تعقیبی توکی با سطح معنی­داری 05/0≥P استفاده شد. یافته‌ها: پس از هشت هفته سطح گلوکز، انسولین، وزن بدن، تری­گلیسیرید (TG)، کلسترول تام (TC)، لیپوپروتئین با چگالی پایین کلسترول (LDL-C)، شاخص­های التهابی و محتوای پروتئین FOXO3 به­طور قابل‌توجهی در گروه­های تمرینی کاهش (001/0P=) و محتوای پروتئین AKT و PI3K به­طور معنی­داری در گروه­های تمرینی افزایش یافت (001/0P=). نتیجه‌گیری: به نظر می‌رسد که هر دو نوع پروتکل تمرین ورزشی با تعدیل سطوح انسولین، گلوکز خون، پروفایل لیپیدی، عوامل التهابی و همچنین افزایش محتوای پروتئینی PI3K و AKT و کاهش محتوای پروتئین FOXO3 در موش‌های تغذیه‌شده با HFD نقش مهمی در کنترل عوامل و مسیرهای وابسته به نارسایی قلبی در موش­های چاق داشتند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigating the effect of high-intensity interval training and moderate-intensity continues training on the protein content of PI3K, AKT, and FOXO3 in the heart of rats fed a high-fat diet

نویسندگان [English]

  • Roghayyeh Afroundeh 1
  • Mojdeh Khajehlandi 2
  • Ali Khajehlandi 3
1 Associate Professor, Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
2 Postdoctoral Researcher, Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
3 1. Associate Professor, Department of Physical Education and Sports Science, Gachsaran branch, Islamic Azad University, Gachsaran, Iran
چکیده [English]

Aim: Although lack of exercise activity, and a high-fat diet (HFD) cause an imbalance between energy consumption and intake, exercise training prevents and controls obesity. Therefore, the recent study aimed  to compare the effect of high-intensity interval training (HIIT) and moderate-intensity continues training (MICT) on the protein content of PI3K, AKT, and FOXO3 in the heart of rats fed a high-fat diet. Methods: In this experimental study, 20 male Wistar rats (approximate age=five weeks and average weight 110±10) were purchased and randomly placed in four groups of five: healthy control with normal diet (HC), sedentary control with HFD (SC), HIIT with HFD (HIIT), and MICT with HFD (MICT). Eight weeks of HIIT and MICT was performed for two groups, separately.  To induce obesity, the animals were fed a high-fat diet for eight weeks. Western blot method was used to check protein content of PI3K, AKT, and FOXO3. One-way analysis of variance with Tukey's post-hoc test was used to analyze the data (with a significance level of P≤0.05). Results: After eight weeks of MICT and HIIT, blood glucose, insulin, body weight, triglycerides (TG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), inflammatory factors, and FOXO3 protein content significantly decreased (P=0/001), and protein content of AKT as well as PI3K significantly increased (P=0/001) in HIIT and MICT groups compared to the SC group. Conclusion: It seems that both types of exercise training protocols played an important role in controlling factors and pathways related to heart failure by modulating insulin levels, blood glucose, lipid profile, inflammatory factors, as well as increase in protein content of PI3K and AKT and decrease in  protein content of FOXO3 in HFD-fed rats.
 

کلیدواژه‌ها [English]

  • PI3K/AKT
  • FOXO3
  • Inflammation
  • Heart
  • obesity

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 12، شماره 2
مهر 1404
صفحه 137-152

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  • تاریخ دریافت: 30 مهر 1403
  • تاریخ بازنگری: 26 آذر 1403
  • تاریخ پذیرش: 02 بهمن 1403

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