اثر تمرینات ترکیبی استقامتی و مقاومتی بر پروتئین‌های ROCKِ عضلۀ اسکلتی و شاخص مقاومت به انسولین در موش‌های سالمند

مقالات آماده انتشار

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران

2 دانشگاه آزاد اسلامی بجنورد

چکیده

هدف: پروتئین کیناز مرتبط با Rho (ROCK) از خانوادۀ کینازهای سرین- ترئونین می­باشد که در تنظیم متابولیسم گلوکز و حساسیت به انسولین نقش دارد. هدف از مطالعه حاضر بررسی اثر هشت هفته تمرینات ترکیبی هوازی و مقاومتی بر سطوح پروتئین­های ROCK عضله اسکلتی، سطوح سرمی انسولین، گلوکز ناشتای خون (FBS)، شاخص مقاومت به انسولین (HOMA-IR) و وزن بدن در موش­های سالمند ماده بود. مواد و روش­ها: 22 سر رت ماده نژاد ویستار (28-24 ماه، 10/15±50/262 گرم) به شیوۀ تصادفی در دو گروه تجربی و کنترل (11 سر در هر گروه) تقسیم شدند. رت­ها در گروه تجربی در هشت هفته تمرینات ترکیبی هوازی (دویدن روی تردمیل در شدت 60-40 درصد سرعت بیشینه، 60 دقیقه در هر جلسه و بدون شیب تردمیل) و مقاومتی (15 مرتبه بالا رفتن از نردبان ویژه جوندگان در شدت 60-40 درصد آزمون بار بیشینه با فواصل استراحت یک دقیقه­ای، 45 دقیقه در هر جلسه) در روزهای متناوب و در ساعت معینی از روز، پنج روز در هفته شرکت کردند. همۀ رت­ها 48 ساعت پس از آخرین جلسۀ تمرینی تشریح شدند و شاخص­های مورد بررسی با استفاده از روش آزمایشگاهی مناسب مورد ارزیابی قرار گرفت. تجزیه و تحلیل داده ها با استفاده از آزمون تی مستقل و زوجی در سطح معنی داری کمتر از 05/0 انجام شد. یافته­ها: اجرای هشت هفته تمرینات ترکیبی هوازی و مقاومتی با افزایش معنی­دار سطوح ROCK1 عضلۀ تند (001/0=P) و کند انقباض (001/0=P) در گروه تجربی در مقایسه با گروه کنترل همراه بود. علاوه بر این، سطوح ِROCK2 عضلۀ تند (001/0=P) و کند انقباض (001/0=P) نیز افزایشی معنی­دار داشت. ضمن اینکه، کاهش معنی­دار FBS (001/0=P) و وزن بدن (001/0=P) در گروه تجربی در مقایسه با گروه کنترل مشاهده شد. هم­چنین، وزن بدن در گروه تجربی در پس آزمون در مقایسه با پیش آزمون با کاهش معنی­دار همراه بود (004/0=P). نتیجه ­گیری: اگرچه برداشت گلوکز خون به دنبال هشت هفته تمرینات ترکیبی هوازی و مقاومتی در موش­های سالمند ماده بهبود یافت؛ چنین به نظر می­رسد افزایش پروتئین­های ROCK عضلۀ اسکلتی نتوانسته است به بهبود حساسیت به انسولین در پاسخ به تمرینات ورزشی کمک کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of Combined Aerobic and Resistance Exercise Training on ROCK Proteins of Skeletal Muscles and Insulin Resistance Index in Aged Rats

نویسندگان [English]

  • mohammad mostafa Rezvani 1
  • Sadegh Cheragh Birjandi 1
  • Najmeh AlSadat Shojaeian, 1
  • Mostafa Teymouri Kheravi 1
1 Department of physical education, Bojnourd branch, Islamic Azad University, Bojnourd, Iran
2
چکیده [English]

Aim: Rho-Associated Protein Kinase (ROCK) is one of the members of serine-threonine protein kinases playing role in regulation of glucose metabolism and insulin sensitivity. The purpose of this study was to investigate the effect of eight weeks of combined aerobic and resistance training on skeletal muscle levels of ROCKs protein, serum levels of insulin, fast blood sugar (FBS), insulin resistance index (HOMA-IR) and body weight in aged female rats. Methods: Twenty two Wistar rats (24-28 months old, 262.5±15.10 gr) randomly divided in to two groups of experimental and control (11 ones in each). Rats in experimental group participated in eight weeks of combined aerobic (running on treadmill at intensity of 40-60 percentage of maximum velocity, 60 minutes per session, without treadmill incline) and resistance training (15 times of climbing on rodent ladder at intensity of 40-60 percentage of maximum workload with 1 minute of rest interval, 45 minutes per session), at intermittent days and five days per week. All rats were dissected 48 hours after the last training session, and the investigated indicators were evaluated using appropriate laboratory methods. Data analyzed using independent and paired t-test at a significance level of less than 0.05. Results: Eight weeks of combined aerobic and resistance training resulted in significant increases in fast (P=0.001) and slow (P=0.001) twitch muscles levels of ROCK1 in experimental compared to control group. Moreover, ROCK2 levels of fast (P=0.001) and slow (P=0.001) twitch muscles significantly increased following training. In addition, FBS levels (P=0.001) and body weight decreased (P=0.001) significantly in experimental compared to control group. Furthermore, body weight decreased in experimental group in post-test compared to pre-test (P=0.004). Conclusions: Although glucose uptake improved following eight weeks of combined aerobic and resistance training in old female rats; it seems that the increases in skeletal muscle levels of ROCK proteins could not contribute to improvement in insulin sensitivity in response to exercise training.

کلیدواژه‌ها [English]

  • Rho-Associated Kinases
  • Exercise Therapy
  • Insulin Resistance
  • Aging

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انتشار آنلاین از تاریخ 17 دی 1403

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  • تاریخ دریافت: 16 مهر 1403
  • تاریخ بازنگری: 16 دی 1403
  • تاریخ پذیرش: 16 دی 1403

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