تأثیر هشت هفته تمرین مقاومتی با مکمل یاری اسپیرولینا پلاتنسیس بر بیان ژن پارامترهای اکسیداتیو در عضله قلبی رت‌های نر

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه خلیج فارس، بوشهر، ایران.

2 دانشیار، گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه خلیج فارس، بوشهر، ایران

چکیده

هدف: تمرینات مقاومتی و مکمل اسپیرولینا بیان ژن های درگیر در مسیر آنتی اکسیدانی را تغییر می‌دهند. هدف از پژوهش حاضر بررسی اثرات تمرین مقاومتی و مکمل اسپیرولینا بر بیان ژن پارامترهای استرس اکسیداتیو در عضله قلبی رت‌های نر بود. روش شناسی: در این تحقیق کاربردی 32 سر رت نر با وزن 20±150 گرم به چهار گروه کنترل، تمرین مقاومتی، اسپیرولینا و تمرین مقاومتی به همراه اسپیرولینا تقسیم شدند. اسپیرولینا به صورت خوراکی به رت‌ها در گروه‌های اسپیرولینا و تمرین مقاومتی به همراه اسپیرولینا با دوز 200 میلی گرم/کیلوگرم وزن بدن در روز به مدت هشت هفته تجویز شد. پروتکل تمرین مقاومتی شامل هشت هفته بالا رفتن از نردبان به ارتفاع یک متر بود. میزان بیان متغیرهای وابسته سوپراکسید دیسموتاز (SOD)، کاتالاز  (CAT)، گلوتاتیون پراکسیداز (GPx) و مالون دی آلدئید (MDA) مطالعه با استفاده از روش Real-time PCR اندازه‌‌‌گیری گردید. از آزمون تحلیل واریانس دوراهه برای تجزیه و تحلیل داده‌ها در سطح معنی داری (05/0P<) استفاده شد. یافته‌ها: میزان بیان ژن SOD در گروه مکمل (031/0=P) کاهش معناداری یافت اما در گروه‌های تمرین (974/0=P) و تعامل مکمل و تمرین معنادار نبود (093/0=P). میزان کاهش بیان ژن CAT در هیچکدام از گروه‌های مکمل (076/0=P)، تمرین (581/0=P) و تعامل مکمل و تمرین (127/0=P) معنادار نبود. تغییرات در میزان بیان ژن GPx در گروه مکمل (032/0=P) کاهش معناداری پیدا کرد اما در گروه‌های تمرین (326/0=P) و تمرین و مکمل (104/0=P) میزان کاهش بیان ژن معنادار نبود. تغییرات بیان ژن MDA در گروه‌ مکمل (009/0=P) کاهش معناداری یافت درحالیکه در گروه‌های تمرین (416/0=P) و مکمل به همراه تمرین کاهش بیان ژن معنادار نبود (051/0=P). نتیجه‌گیری: نتایج مطالعه حاکی از آن است که مصرف مکمل اسپیرولینا پلاتنسیس به تنهایی در کاهش استرس اکسیداتیو در عضله قلبی رت‌های نر تاثیر دارد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of 8 weeks of resistance training with Spirulina platensis supplement on gene expression of oxidative parameters in the heart muscle of male rats

نویسندگان [English]

  • Atousa Zandvakili 1
  • Abdossaleh zar 2
  • Hamid Reza Sadeghipour 1
1 Department of Sports Sciences, Faculty of Literature and Humanities, Persian Gulf University, Bushehr, Iran.
2 Associate professor, Department of Sport Science, School of Literature and Humanities, Persian Gulf University, Boushehr, Iran
چکیده [English]

Aim: Resistance training and spirulina supplementation change the expression of genes involved in the antioxidant pathway. The purpose of this study was to investigate the effects of resistance training and spirulina supplementation on the gene expression of some oxidative stress parameters in the heart muscle of male rats. Methods: 32 male rats weighing 150±20 grams were divided into four groups, which included control (CO), resistance training (RT), spirulina (SP), and resistance training with spirulina (RTS). Spirulina was orally administered to rats in SP and RTS groups at a dose of 200 mg/kg per day. The resistance training protocol consisted of eight weeks of climbing a 1-meter high ladder. The expression level of the dependent variables (SOD, CAT, GPx, and MDA) of the study was measured using the Real-time PCR method. We used of Two-way ANOVA in SPSS (P<0.05). Results:  The level of SOD gene expression decreased significantly in the supplement group (P=0.031), but in the training groups (P=0.974) and the interaction between supplementation and exercise was not significant (P=0.093). The decrease in CAT gene expression was not significant in any of the supplement (P=0.076), exercise (P=0.581) and the interaction of supplement and exercise (P=0.127) groups. The changes in GPx gene expression in the supplement group (P=0.032) decreased significantly, but in the exercise (P=0.326) and exercise and supplement (P=0.104) groups, the decrease in gene expression was not significant.  MDA gene expression changes were significantly reduced in the supplement group (P=0.009), while there was no significant decrease in gene expression in the exercise (P=0.416) and supplement plus exercise groups (P=0.051). Conclusions: The results of the study indicate that the consumption of spirulina platensis supplement alone has an effect in reducing oxidative stress in the heart muscle of male rats. 

کلیدواژه‌ها [English]

  • Resistance training
  • Spirulina
  • Cardiac muscle

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 11، شماره 2
مهر 1403
صفحه 141-153

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  • تاریخ دریافت: 24 اسفند 1402
  • تاریخ بازنگری: 14 خرداد 1403
  • تاریخ پذیرش: 14 خرداد 1403

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