The effect of eight weeks aerobic exercise training and zinc supplementation on some hepatokines and hepatic enzymes in male Wistar rats intoxicated with Bisphenol-A

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Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, Iran

2 Department of Internal Diseases and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract

Aim:      Bisphenol-A (BPA) is a monomer that is used in the production of the protective resin layer in polycarbonate plastics and has a negative effect on the endocrine system. The purpose of the present study was to determine the effect of eight weeks of aerobic exercise training with nanoparticle and zinc oxide supplementation on hepatic enzymes and some hepatokines (Fetuin-A and FGF-21) in rats intoxicated with BPA. Methods: The present study was experimental.  60 male rats were divided into 12 groups: 1) Control; 2) BPA; 3) Exercise; 4) Exercise+BPA; 5) Nanoparticle supplementation (Nano); 6) Nanoparticle+BPA (Nano+BPA); 7) Zinc oxide salt (Zno); 8) Zinc oxide salt+BPA (Zno+BPA); 9) Exercise+Nano; 10) Exercise+Nano+BPA; 11) Exercise+Zno; and 12) Exercise+Zno+BPA. Aerobic training was performed for eight weeks, five sessions each week with 50% to 75% VO2max, and 25−64 minutes each. Nanoparticle and zinc oxide supplements (3 mg/kg) were injected into animals five days a week for eight weeks. The ALT, AST, Fetuin-A, and FGF-21 levels were measured by ELISA method. One-way ANOVA was used to compare between groups and Tukey's test was used to determine the variety between groups. Results: BPA caused a significant increase in ALT, AST and Fetuin-A activity compared to the control group (p=0.001), but had no effect on FGF-21 levels (p>0.05). On the other hand, the supplement alone and the combination of the supplement with exercise led to a significant decrease in ALT and AST activity (p=0.001). Exercise alone, supplements alone, and their combination also led to a significant decrease in Fetuin-A (p=0.001). Conclusions: Aerobic exercise, zinc supplementation, and especially the combination of these two (aerobic exercise + zinc supplementation) can probably reduce the negative effects induced by BPA.

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References

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Journal of Applied Health Studies in Sport Physiology

Articles in Press, Accepted Manuscript
Available Online from 13 October 2023

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  • Receive Date: 13 July 2023
  • Revise Date: 12 October 2023
  • Accept Date: 12 October 2023

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