تاثیر تمرینات تناوبی شدید همراه با مکمل خرفه بر FXR و SREBP-1cبافت کبد رت های مبتلا به کبد چرب غیر الکلی

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه تربیت بدنی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران

2 گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه نیشابور، نیشابور، ایران

3 گروه تربیت بدنی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران

چکیده

هدف: فعالیت بدنی و مصرف مکمل های دارویی یکی از استراتژی‌های درمانی برای مدیریت کبد چرب غیر الکلی است. هدف پژوهش حاضر بررسی تاثیر تمرینات تناوبی شدید (HIIT) همراه با مکمل خرفه بر FXR، SREBP-1c بافت کبد رت‌های مبتلا به کبد چرب غیر الکلی بود. روش‌شناسی:  در این پژوهش تجربی 30 سر موش‌صحرایی نژاد ویستار نر با دامنه وزنی 185-160 گرم به طور تصادفی در پنج گروه کنترل سالم(5=n)، کنترل کبد چرب(5=n)، مکمل خرفه(5=n)، تمرین تناوبی شدید(5=n) و تمرین تناوبی شدید+مکمل خرفه(5=n) تقسیم شدند. کبدچرب‌غیرالکلی با 12 هفته رژیم غذایی پرچرب ایجاد گردید. پس از تایید ابتلا به کبدچرب‌غیرالکلی مکمل خرفه بصورت روزانه و تمرین تناوبی شدید به مدت 8 هفته، 5 جلسه در هفته در گروه‌های مربوطه اعمال شد. مقادیر FXR و SREBP-1c بافت کبد به روش الایزای ساندویچی اندازه‌گیری شد. به منظور تجزیه و تحلیل آماری داده‌ها از آزمون آنالیز واریانس یک طرفه استفاده شد. یافته‌ها: پس از 8 هفته تمرین HIIT و مصرف مکمل خرفه تفاوت معناداری در مقادیر FXR (028/0=p) و SREBP-1c (005/0=p) بین گروه های مطالعه وجود داشت. نتایج آزمون تعقیبی نشان داد که مقادیر SREBP-1c در گروه کنترل کبد چرب نسبت به گروه کنترل سالم به طور معناداری بالاتر بود (012/0=p). مقادیر SREBP-1c در گروه تمرین + مکمل خرفه (006/0=p) و گروه تمرین تناوبی(049/0=p) نسبت به گروه کنترل کبد چرب به طور معناداری پایین‌تر بود. مقادیر FXR در گروه کنترل کبد چرب نسبت به گروه کنترل سالم به طور معناداری پایین‌تر بود (027/0=p). تفاوت معناداری در مقادیر FXR در گروه‌های تمرین تناوبی+ مکمل خرفه(462/0=p)، تمرین تناوبی (958/0=p) و مکمل خرفه (988/0=p) نسبت به گروه کنترل کبد چرب مشاهده نشد. نتیجه‌گیری: تمرین تناوبی شدید همراه با مصرف مکمل خرفه از طریق تعدیل برخی مسیرهای تنظیم لیپوژنز کبد ازجمله کاهش فعالیت SREBP-1c بافت کبد ممکن است در بهبود کبدچرب غیرالکلی موثر باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of high intensity interval training with Portulaca Oleracea supplementation on FXR and SREBP-1c in the liver tissue of rats with non-alcoholic fatty liver disease

نویسندگان [English]

  • Majid Farahnia 1
  • Mohammad Raza Hosseinabadi 1
  • Mehdi Zarei 2
  • Mahmoud Soltani 3
1 Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, I.R. Iran
2 Department of Physical Education and Sport Sciences, Faculty of Literature and Humanities, University of Neyshabur, Neyshabur, I.R. Iran
3 Department of Physical Education, Mashhad Branch, Islamic Azad University, Mashhad, I.R. Iran
چکیده [English]

Aim:   Physical activity and the use of medicinal supplements are one of the treatment strategies for managing non-alcoholic fatty liver disease. The purpose of this study is to investigate the effect of high intensity interval training (HIIT) along with Portulaca Oleracea supplement on FXR and SREBP-1c in the liver tissue of rats with non-alcoholic fatty liver. Methods: In this experimental study, 30 male Wistar rats were randomly divided into five groups: healthy control (n=5), fatty liver control (n=5), Portulaca Oleracea supplement (n=5), HIIT (n=5) and HIIT + Portulaca Oleracea supplement (n=5). Nonalcoholic fatty liver was induced with 12 weeks of high-fat diet. Portulaca Oleracea supplement was applied daily and HIIT training for 8 weeks, 5 sessions per week in the respective groups. The amounts of FXR and SREBP-1c in liver tissue were measured by sandwich ELISA method. One-way analysis of variance was used to analyze the data. Results:  After 8 weeks of HIIT and Portulaca Oleracea supplementation, there was a significant difference in FXR (p=0.028) and SREBP-1c (p=0.005) values between the study groups. The results of the post hoc test showed that the SREBP-1c levels in the fatty liver control group were significantly higher than the healthy control group(p=0.012). SREBP-1c values were significantly lower in the HIIT + Portulaca Oleracea supplement group (p=0.006) and the exercise group (p=0.049) compared to the fatty liver control. FXR values in the fatty liver control group were significantly lower than the healthy control group(p=0.027). There was no significant difference in FXR values in the groups of HIIT + Portulaca Oleracea supplement (p=0.462), HIIT (p=0.958) and Portulaca Oleracea supplement (p=0.988) compared to the fatty liver control. Conclusion: HIIT with Portulaca Oleracea supplementation may be effective in improving non-alcoholic fatty liver disease by modulating some liver lipogenesis regulation pathways, including reducing SREBP-1c activity in liver tissue.
 

کلیدواژه‌ها [English]

  • High-intensity interval training
  • Portulaca
  • Non-alcoholic fatty liver disease
  • FXR
  • SREBP-1c

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 11، شماره 1
فروردین 1403
صفحه 124-138

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  • تاریخ دریافت: 04 شهریور 1402
  • تاریخ بازنگری: 18 مهر 1402
  • تاریخ پذیرش: 19 مهر 1402

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