اثر هشت هفته تمرین تناوبی شدید و تداومی با شدت متوسط بر بیان ژن SIRT3 و HSP70 بافت قلب رت‌های نر نژاد اسپراگ-داولی چاق شده با غذای پر چرب و مبتلا به دیابت

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشجوی دکتری ، گروه تربیت بدنی و علوم ورزشی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران

2 گروه تربیت بدنی، دانشکده علوم انسانی، دانشگاه آزاد اسلامی واحد سنندج، سنندج، ایران

3 گروه تربیت بدنی، دانشکده علوم انسانی، دانشگاه آزاد اسلامی واحد مرودشت، مرودشت، ایران

10.22049/jahssp.2023.28232.1536

چکیده

هدف: تاثیر تمرین ورزشی در کنترل بیماری دیابت اثبات شده است، اما تفاوت بین نوع تمرین به ویژه  تداومی با شدت متوسط و تناوبی شدید بر مکانیسم های محافظتی بافت قلب هنوز به خوبی شناخته نشده است. لذا هدف از مطالعه حاضر مقایسه اثر هشت هفته تمرین تناوبی شدید و تداومی با شدت متوسط بر بیان ژن SIRT3 و HSP70 بافت قلب رت های چاق شده با غذای پر چرب و مبتلا به دیابت بود. روش شناسی: 18 سر رت نر نژاد اسپراگ-داولی به مدت سه ماه تحت رژیم غذایی پرچرب و سپس تزریق صفاقی mg/kg 30 استروپتوزوتوسین قرار گرفتند. رت ها بر اساس گلوکز خون در 3 گروه 6 تایی، تمرین تناوبی شدید، تمرین تداومی با شدت پایین، و کنترل تقسیم شدند. همچنین 6 سر رت برای بررسی اثر رژیم غذای پرچرب و دیابت بر متغیر های تحقیق در گروه کنترل سالم بررسی شدند. طول پروتکل تمرینات 8 هفته بود که طی آن گروه تمرین تناوبی در هفته اول 6 و در هفته آخر 12 وهله تمرینی را با شدت 80 تا 85 درصد حداکثر سرعت دویدن انجام دادند. تمرین گروه تمرین تداومی با شدت متوسط شامل دویدن 50 تا 55 درصد حداکثر سرعت بود، که از 25 دقیقه هفته اول به 50 دقیقه در هفته پایانی رسید. در انتها مقادیر بیان ژن SIRT3 و HSP70 در بافت قلب به روش qReal Time PCR اندازه گیری شد. همچنین جهت تحلیل یافتهها از آزمون آنالیزواریانس (ANOVA) یکراهه استفاده شد (05/0>P). یافته ها: مقادیر بیان ژن SIRT3 در گروههای تدامی با شدت متوسط و تناوبی شدید به طور معنیداری بالاتر از گروه کنترل بود (001/0=P). همچنین مقادیر بیان ژن HSP70 در گروه تناوبی شدید به طور معنیداری پایینتر از گروه کنترل بود (01/0=P). تفاوت معنیداری در مقادیر SIRT3 و HSP70 در تمرین تناوبی شدید و تداومی مشاهده نشد (05/0≤P).  نتیجه گیری: به نظر میرسد فعالیتهای ورزشی تداومی و تناوبی با افزایش بیان SIRT3 در بهبود عملکرد میتوکندریایی نقش دارند؛ اما با توجه کاهش مقادیر HSP70 در گروه تمرینات تناوبی شدید انجام مطالعات بیشتر در ارتباط با تاثیر تمرینات شدید بر دیگر مکانیسمهای مزتبط با نشانگرهای حفاظتی سلول لازم است.

کلیدواژه‌ها


عنوان مقاله [English]

Title: The effect eight weeks of high intensity interval training and moderate intensity continuous training on the gene expression of SIRT3 and HSP70 in heart tissue of diabetic male Sprague Dawley rats obesed by high-fat diet.

نویسندگان [English]

  • Meysam Rozestan 1
  • Khalid Mohamadzadeh Salamat 2
  • Seyed Ali Hoseini 3
1 PhD student, Department of Physical Education and Sports Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
2 Department of Physical Education, Faculty of Humanities, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran
3 Department of Physical Education, Faculty of Humanities, Islamic Azad University, Marodasht Branch, Marodasht, Iran
چکیده [English]

Aim:       The effect of exercise training in control of diabetes has been proved, but comparison of the effect of high intensity interval training (HIIT) and moderate intensity continuous training (MICT) on the protective mechanisms of heart tissue is still not well known. So, the aim of this study was to compare the effect of HIIT and MICT on the Sirtuin-3 (SIRT3) and Heat shock protein-70 (HSP70) gene expression in heart tissue of high-fat diet and diabetic rats. Methods: eighteen male Sprague Dawley rats were fed with high-fat diet for three months. After that, diabetes was induced by injecting 30 mg/kg streptozotocin intra peritoneally. Then the rats were divided into 3 groups of 6 rats including, HIIT, MICT, and control. Also, 6 rats were considered to investigate the effect of high-fat diet and diabetes on research variables in the healthy control group. During 8 weeks training protocol, the HIIT group performed 6 training sessions in the first week to 12 training sessions in the last week training sessions with intensity of 80-85% of the maximum speed. MICT group was run 50 to 55% of the maximum running speed which lasted from 25 minutes in the first week to 50 minutes in the final week. Finally, SIRT3 and HSP70 gene expression values ​​were measured in heart tissue by qRT-PCR method. One-way analysis of variance (ANOVA) test was used to analyze the data (P<0.05). Findings: SIRT3 gene expression levels in the HIIT and MICT groups were significantly higher than the control group (P=0.001) and HSP70 gene expression levels in the HIIT group were lower than the control group (P=0.01). In addition, no significant difference was observed in SIRT3 and HSP70 values ​​in HIIT and MICT training (P≤0.05). Conclusion: It seems that HIIT and MICT play a role in improving mitochondrial function by increasing SIRT3 expression; However, considering the decrease in HSP70 levels following HIIT, it seems that more studies with the approach of cell damage following high-intensity training are needed.

کلیدواژه‌ها [English]

  • Exercise Training
  • SIRT
  • HSP70
  • Heart
  • Diabetes Mellitus
  1. 1. Dastah, Samaneh, Babaei Bonab, Solmaz. The effect of exercise in water on the amount of Fetuin-A, ANGPTL4 and FGF21 in the serum of obese women with type 2 diabetes. Applied health studies in exercise physiology, 2021; 8(2): 51-60. doi: 10.22049/jahssp.2021.27436.1390

    1. Segula D. Complications of obesity in adults: a short review of the literature. Malawi Med J. 2014;26(1):20–4.
    2. Oraii A, Shafiee A, Jalali A, Alaeddini F, Saadat S, Masoudkabir F, et al. Prevalence, awareness, treatment, and control of type 2 diabetes mellitus among the adult residents of tehran: Tehran Cohort Study. BMC Endocr Disord. 2022;22(1):1–15.
    3. Davari F, Alimanesh Z, Alimanesh Z, Salehi O, Hosseini SA. Effect of training and crocin supplementation on mitochondrial biogenesis and redox-sensitive transcription factors in liver tissue of type 2 diabetic rats. Arch Physiol Biochem. 2020;1–6.
    4. Sharafi Dehrhm F, Soori R, Rastegar MM M, Sadegh A. The effect of high intensity interval training on muscular biomarkers of mitochondrial biogenesis in male rats. J Babol Univ Med Sci. 2017;19(6):57–63. [In Persian]
    5. Nikozadeh, Alireza, Pozesh Jadidi, Ruqieh, Noorazar, Mir Alireza, Asgharpour Arshad, Masoud, Bashiri, Jabbar. The interactive effect of aerobic exercise and curcumin supplementation on mitochondrial NRF-1 and NRF-2 gene expression in cardiomyocytes of male rats model of myocardial infarction. Applied health studies in exercise physiology, 2021; 8(2): 44-50. doi: 10.22049/jahssp.2021.27321.1370

    6. Dadrass A, Mohamadzadeh Salamat K, Hamidi K, Azizbeigi K. Anti-inflammatory effects of vitamin D and resistance training in men with type 2 diabetes mellitus and vitamin D deficiency: a randomized, double-blinded, placebo-controlled clinical trial. Journal of Diabetes & Metabolic Disorders. 2019;18:323-31.[In Persian]
    7. Fathi I, Nourshahi M, Haghparast A, Fallahi HH. Effect of eight-week aerobic continuous and high intensity interval training on levels of SIRT3 in skeletal muscle tissue of Wistar rats. JSEP. 2015; 1277-1289. [In Persian]
    8. Hodge T, Starnes J, Feger B, Hixson L, Harris MB. Effects of exercise and body temperature on eNOS, SIRT1, SIRT3 and Hsp70 expression in rat plantaris muscles (1164.6). FASEB J. 2014;28:1164–6.
    9. Tashakori Zade M, Mogharnasi M. A study of the effect of 10 weeks of resistance training on HSP70 and insulin resistance in type 2 diabetic women. J Sport Biosci. 2016;8(3):341–51. [In Persian]
    10. Nameni F. The Effect of a Single Bout Endurance Training on HSP70. World Appl Sci J. 2012;19(2):211–4.
    11. Palacios OM, Carmona JJ, Michan S, Chen KY, Manabe Y, Ward III JL, et al. Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1α in skeletal muscle. Aging (Albany NY). 2009;1(9):771.
    12. Saremi A. Sporting exercises and diabetes mellitus type 2: a review on evidences. Cell and Tissue. 2011; 2(3): 171-181. [In Persian]
    13. Huynh FK, Muoio DM, Hirschey MD. SIRT3 directs carbon traffic in muscle to promote glucose control. Diabetes. 2015;64(9):3058–60.
    14. Tabari E, Mohebbi H, Karimi P, Moghaddami K, Khalafi M. The effects of interval training intensity on skeletal muscle pgc-1α in type2 diabetic male rats. Iran J Diabetes Metab. 2019;18(4):179–88. [In Persian]
    15. Davari F, Alimanesh Z, Alimanesh Z, Salehi O, Hosseini SA. Effect of training and crocin supplementation on mitochondrial biogenesis and redox-sensitive transcription factors in liver tissue of type 2 diabetic rats. Arch Physiol Biochem. 2022;128(5):1215–20.
    16. Zanjirian Z, Sarir H, Mohammadnia Ahmadi M. Effect of Continuous Exercise Training on Protein Levels of SIRT3 and OGG1 in the Liver Tissue of Male Wistar Rats. J Ilam Univ Med Sci. 2019;27(5):97–107. [In Persian]
    17. Lanza IR, Short DK, Short KR, Raghavakaimal S, Basu R, Joyner MJ, et al. Endurance exercise as a countermeasure for aging. Diabetes 2008; 57: 2933–2942. Diabetes. 2012;61(10):2653.
    18. Gurd BJ, Holloway GP, Yoshida Y, Bonen A. In mammalian muscle, SIRT3 is present in mitochondria and not in the nucleus; and SIRT3 is upregulated by chronic muscle contraction in an adenosine monophosphate-activated protein kinase–independent manner. Metabolism. 2012;61(5):733–41.
    19. Asadi RHK, Arshadi S, Far AAB, Rasouli MH. The effect of 12-week aerobic trainings on mitochondrial biogenesis indicators in skeletal muscle among male rats. Majallah-i pizishki-i Danishgah-i Ulum-i Pizishki va Khadamat-i Bihdashti-i Darmani-i Tabriz. 2020;42(3):348–55. [In Persian]
    20. Hokari F, Kawasaki E, Sakai A, Koshinaka K, Sakuma K, Kawanaka K. Muscle contractile activity regulates Sirt3 protein expression in rat skeletal muscles. J Appl Physiol. 2010;109(2):332–40.
    21. Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial sirtuin deacetylase, regulates mitochondrial function and thermogenesis in brown adipocytes. J Biol Chem. 2005;280(14):13560–7.
    22. Sharifi E, Askari R, Haghighi AH. Comparision the effects of high and average intensity combined training on levels of serum heat shock proteins 70 and glycemic control in obese women with type 2 diabetes. Jundishapur Sci Med J. 2017;16(2):255–65. [In Persian]
    23. Kandi Asadi RH, Arshadi S, Banaei Far AA, Rasouli MH. The effect of 12-week aerobic trainings on mitochondrial biogenesis indicators in skeletal muscle among male rats. Med J Tabriz Univ Med Sci Heal Serv. 2020;42(3): 348-355. [In Persian]
    24. Pouzesh Jadidi G, Seify F, Bolboli L, Pourrahim A. Effect of high intensity interval training on cardiomyocytes HSP70 and Caspase-3 gene expression levels in myocardial infarction male rat model. J Appl Heal Stud Sport Physiol. 2022;9(2):1–11. [In Persian]
    25. Abdollahi S, Mohamadzadeh SK, Azizbeigi K, Etemad Z. The Effect of aerobic training and octopamine on HSP70 and Caspase-3 protein expression in brown adipose tissue in rats received deeply heated oil treatment. 2020; 8(1): 48-59. [In Persian]
    26. Sulzbacher MM, Ludwig MS, Heck TG. Oxidative stress and decreased tissue HSP70 are involved in the genesis of sepsis: HSP70 as a therapeutic target. Rev Bras Ter intensiva. 2020;32:585–91.
    27. Szyller J, Bil-Lula I. Heat shock proteins in oxidative stress and ischemia/reperfusion injury and benefits from physical exercises: A review to the current knowledge. Oxid Med Cell Longev. 2021;2021.