اثر تمرین هوازی و قهوه سبز بر ترموژنز بافت چربی موش های پیش دیابتی

مقالات آماده انتشار

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی دانشگاه سیستان و بلوچستان، زاهدان، ایران

2 استادیار گروه علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه سیستان و بلوچستان، زاهدان، ایران

3 گروه تربیت بدنی و علوم ورزشی دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران

4 گروه تربیت بدنی و علوم ورزشی، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران

5 استاد جنین شناسی، پژوهشکده زیست فناوری رویان، اصفهان، ایران

چکیده

هدف: پیش دیابت یک شرایط پرخطر برای ابتلا به دیابت نوع2 است و بر روی متابولیسم چربی و گلوکز تأثیر به سزایی دارد. هدف از پژوهش حاضر، تعیین اثر تمرین هوازی و قهوه سبز بر بیان ژن­های ترموژنیک پروتئین جفت نشده1(UCP1) و یدوتیرونین دیودیناز2(DIO2)در بافت چربی زیرپوستی موش­های پیش دیابتی است. روش پژوهش: از تعداد 25سر موش نر نژادC57bl-6، با سن4 هفته، 5 سر به­عنوان گروه کنترل و 20 سر برای گروه پیش دیابت در نظر گرفته شدند. در مرحله اول به­مدت 12هفته به گروه کنترل رژیم غذایی نرمال و به گروه پیش دیابت رژیم غذایی پرچرب داده­شد. پس از القای پیش دیابت، در فاز دوم مطالعه موش­ها به­صورت تصادفی به گروه­های:کنترل(Ctrl)، پیش دیابت(PD)، پیش دیابت-تمرین هوازی(EX)، پیش دیابت-قهوه سبز(GC) و پیش دیابت-قهوه سبز-تمرین هوازی(EX/GC) تقسیم شدند. موش­های گروه‌EX و EX/GC به­مدت ۱۰هفته بر روی تردمیل دویدند. همچنین موش­ها در گروه­های EX/GC و GC به­مدت 10هفته تحت گاواژ قهوه سبز قرار گرفتند. بیان ژن­های UCP1 و DIO2 در بافت چربی زیرپوستی به روش Real time PCR اندازه­گیری شدند و پس از کمّی سازی، مقادیر بیان ژن با استفاده از فرمول ΔΔCt-2 و سپس آزمونt، آنووا و آزمون تعقیبی توکی تجزیه و تحلیل شدند. یافته‌ها: نتایج نشان داد12هفته رژیم پرچرب باعث افزایش قند خون ناشتا شد و موش­ها را در شرایط پیش دیابت قرار داد، همچنین بیان نسبیUCP1 و DIO2 در بافت چربی زیرپوستی گروهEX نسبت به گروه­های دیگر افزایش معناداری داشت(000/0=P). نتیجه‌گیری: نتایج نشان داد که تمرین هوازی و قهوه سبز می­توانند اثرات مفیدی بربیان ژن­هایUCP1 و DIO2 در بافت چربی زیرپوستی موش­های پیش دیابتی داشته و ممکن است ترموژنز را در این بافت فعال کنند و از این طریق، خطرات ناشی از پیش دیابت را کاهش دهند.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Aerobic Exercise and Green Coffee on Adipose Tissue Thermogenesis in Pre-Diabetic Mice

نویسندگان [English]

  • Fateme Jangharbani 1
  • Abbas Salehi Kia 2
  • Seyed Mohammad Marandi 3
  • Milad Abdollahi 4
  • Mohammad Hossein Nasr-Esfahani 5
1 Department of Physical Education and Sport Science ,University of Sistan and Baluchistan, Zahedan, Iran
2 Department of Sports Sciences, Faculty of Educational Sciences and Psychology, University of Sistan and Baluchistan, Zahedan, Iran
3 Professor of Sports Sciences Department, Faculty of Sports Sciences, Isfahan University,Isfahan, Iran
4 Department of Physical Education and Sport Science , Isfahan University, Isfahan, Iran
5 Professor of Embryology, Royan Biotechnology Research Institute, Isfahan, Iran
چکیده [English]

Aim: Prediabetes is a high-risk condition for type2diabetes and has a effect on fat and glucose metabolism. The aim of this study was to determine the effect of aerobic exercise and green coffee on the expression of thermogenic genes of uncoupling protein1(UCP1) and iodothyronine deiodinase2(DIO2) in the adipose tissue of prediabetic mice. Methods: Out of 25 male C57bl-6 mice aged 4 weeks, 5 were considered as control group and 20 were considered as prediabetes group. In the first stage, the control group was given a normal diet and the prediabetic group was given a high-fat diet for 12 weeks. After induction of prediabetes, in the second phase of the study, mice were randomly divided into groups: control(Ctrl), prediabetes(PD), prediabetes-aerobic exercise(EX), prediabetes-green coffee(GC) and prediabetes-green coffee-aerobic exercise(EX/GC). Mice of EX and EX/GC groups ran on a treadmill for 10 weeks. Also, mice in EX/GC and GC groups were subjected to green coffee gavage for 10 weeks. The expression of UCP1 and DIO2 genes in subcutaneous fat tissue was measured by real time PCR method and after quantification, gene expression values were analyzed using ΔΔCt-2 formula and then t-test, ANOVA and Tukey's post hoc test. Results: The results showed that12weeks of high-fat diet increased fasting blood sugar and exposed mice to prediabetes. Also, the relative expression of UCP1 and DIO2 in adipose tissue of EX group was significantly increased compared to other groups(P=0/000). Conclusion: The results showed that aerobic exercise and green coffee can have beneficial effects on the expression of UCP1 and DIO2 genes in the subcutaneous adipose tissue of prediabetic mice and may activate thermogenesis in this tissue and thereby reducing the risks of prediabetes.

کلیدواژه‌ها [English]

  • Prediabetes
  • Aerobic exercise
  • Green coffee
  • Thermogenesis
  • Subcutaneous adipose tissue

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  • تاریخ دریافت: 06 دی 1401
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