اثر طول دوره مصرف کورکومین به همراه تمرینات HIIT بر تعادل آنتی‌اُکسیدانی عضلۀ اسکلتی، قلب و کبد رت‌های نر

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان، ایران

2 گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان

3 گروه فیزیولوژی ورزشی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

هدف: چالش اصلی ورزشکاران در اجرای تمرینات شدید، بروز فشار اکسایشی در بافت­های مختلف است. از این رو، هدف پژوهش حاضر بررسی اثر مصرف مکمل کورکومین در طول هشت هفته و 48 ساعت پایانی تمرین تناوبی شدید (HIIT) بر میزان فعالیت گلوتاتیون پراکسیداز (GPX) و مالون­دی­آلدئید (MDA) عضلة اسکلتی، قلب و کبد موش­های صحرایی نر ویستار بود. روش شناسی: در این پژوهش تجربی، تعداد 42 سر موش صحرایی نر ویستار با سن هشت هفته پس از یک هفته آشناسازی، به شش گروه (کنترل، کورکومین، 48 ساعت کورکومین، تناوبی، تناوبی+کورکومین و تناوبی+48ساعت کورکومین)، تقسیم شدند. تمرین تناوبی شدید شامل هشت هفته و پنج جلسه در هفته دویدن روی نوارگردان مخصوص جوندگان بود. مکمل کورکومین به­میزان 30 میلی­گرم به ازای هر کیلوگرم از وزن بدن به شکل داخل صفاقی (سه جلسه در هفته) یا (48 ساعت پایانی، هر 8 ساعت) تزریق شد. سطوح فعالیت GPX با روش الایزا و MDA با روش اسپکتروفتومتری اندازه­گیری شد. داده­ها با تحلیل واریانس یک­طرفه تحلیل شدند. یافته­ها: فعالیت GPX عضلة اسکلتی، قلب و کبد گروه تناوبی در مقایسه با گروه کنترل به­طور معنا­داری (05/0>P) کمتر و سطوح MDA در بافت قلب به‌طور معنا­داری (001/0=P) بالاتر بود. همچنین، میزان فعالیت GPX در عضلة اسکلتی (001/0=P)، قلب (004/0= P) و کبد (029/0= P) گروه تناوبی+کورکومین و همچنین قلب(008/0= P) تناوبی+48 ساعت کورکومین به‌طور معناداری از گروه تناوبی بیشتر بود. علاوه­براین، میزان سطوح MDA قلب در گروه­های تناوبی+کورکومین (001/0P =) و تناوبی+48 ساعت کورکومین (001/0P =) از گروه تناوبی کمتر بود. نتیجه­گیری: با وجود پاسخ­های اکسایشی متفاوت بافت­ها به تمرینات HIIT، مصرف مکمل کورکومین در 48 ساعت پایانی می­تواند همانند مصرف آن در طول هشت هفته از اثرات فشار اکسایشی جلوگیری کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Intake Duration of Curcumin Supplementation along with HIIT on Antioxidant Balance of Skeletal Muscle, Heart, and Liver in Male Rats

نویسندگان [English]

  • Ali Gorzi 1
  • Ahmad Rahmani 2
  • Samane Ekradi 3
1 Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran.
2 Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran.
3 Department of Exercise Physiology, University of Tarbiat Modarres, Tehran, Iran.
چکیده [English]

Aim:  Oxidative stress in different organs is the main challenge for athletes who undergo strenuous training. This study investigated the effect of curcumin supplementation along with HIIT on the antioxidant balance of skeletal muscle, heart, and liver in male Wistar rats. Methods: Forty two male Wistar rats aged six-eight weeks, after one week familiarization period, were randomly divided into 6 groups; Control, Curcumin, 48hrs Curcumin, HIIT, HIIT+Curcumin, and HIIT+48hrs Curcumin. HIIT training (8 weeks, 5 sessions a week) carried out on a rodent treadmill. Curcumin supplement (30 mg/kg.BW) injected Intraperitoneally (eight weeks, three times a week; or last 48 hours, every 8 hours) in the supplemented groups. GPX activity was measured by Elisa Kit and the MDA level was assessed by spectrophotometric method. Results: One-way ANOVA results showed that GPX activity of the HIIT group in skeletal muscle, heart, and liver were significantly (P<0.05) lower, and MDA levels of the heart were significantly (P=0.001) higher than the control group. Also, GPX activity of skeletal muscle (P=0.001), heart (P=0.004), and liver (P=0.029) in the HIIT+curcumin and HIIT+48hrs curcumin group of the heart (P=0.008) were significantly higher than HIIT group. MDA levels of heart in the HIIT+curcumin (p=0.001) and HIIT+48hrs curcumin (P=0.001) groups were significantly lower than the HIIT group. Conclusion: In spite of different oxidative stress responses of tissues to the HIIT training, curcumin supplementation during the last 48 hours of strenuous HIIT, as well as during eight weeks, could prevent oxidative stress, and improves antioxidant capacity.

کلیدواژه‌ها [English]

  • Curcumin
  • High Intensity Interval Training
  • Antioxidant Balance

Publisher: Azarbaijan Shahid Madani University          Copyright © The Authors   

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 10، شماره 1
فروردین 1402
صفحه 112-126

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  • تاریخ دریافت: 26 آبان 1401
  • تاریخ بازنگری: 28 بهمن 1401
  • تاریخ پذیرش: 30 بهمن 1401

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