تأثیر هشت هفته تمرینات تناوبی سرعتی (SIT) بر وضعیت اکسیدانی/آنتی‌اکسیدانی بافت قلب رت‌های نر ویستار تحت رژیم غذایی پرکالری با نمک زیاد

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی ورزش، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه الزهرا، تهران، ایران.

2 دانشیار فیزیولوژی ورزش، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه الزهرا، تهران، ایران.

3 دانشیار فیزیولوژی ورزش، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه شاهد، تهران، ایران.

چکیده

هدف: از عواملی که باعث افزایش تولید رادیکال­های آزاد و استرس اکسایشی در قلب می­شود، مصرف رژیم پرکالری با نمک زیاد است. هدف از این مطالعه بررسی تأثیر هشت هفته تمرینات تناوبی سرعتی (SIT) بر برخی وضعیت اکسیدانی/آنتی­اکسیدانی در بافت قلب رت­های نر ویستار تحت رژیم غذایی پرکالری با نمک زیاد بود. روش شناسی: این مطالعه از نوع تجربی بود که در آن 32 سر رت ­نر نژاد ویستار به­صورت تصادفی به چهار گروه هشت­تایی کنترل با رژیم نرمال (ND)، رژیم عذایی نرمال و تمرین (ND/SIT)، رژیم پرکالری با نمک زیاد (HCL/HS) و تمرین+رژیم پرکالری با نمک ­زیاد (HCL/HS +SIT) تقسیم شدند. تمرین SIT به مدت هشت هفته، 3 روز در هفته، 4-9 تکرار در هر جلسه اجرا شد. پس از  48 ساعت از آخرین جلسه تمرین بافت­برداری از قلب انجام گردید و برای سنجش فعالیت آنزیم­های سوپراکسیددیسموتاز (SOD) و گلوتاتیون پراکسیداز (GPX)، و ظرفیت تام آنتی­اکسیدانی (TAC) و مقادیر مالون­دی­آلدهید (MDA) استفاده شد. یافته‌ها: براساس نتایج نشان داد هشت هفته تمرین SIT موجب افزایش معنادار فعالیت آنزیم­های SOD و GPX در گروه HCL/HS+SIT و همچنین کاهش معنی­دار سطح MDA در بافت قلب شد (001/0=p). اما در میزان TAC پس از انجام هشت هفته تمرین SIT در گروه HCL/HS+SIT تغییر معنی­داری ایجاد نشد (148/0=p). نتیجه‌گیری: باتوجه به یافته­های پژوهش حاضر، به­نظر می­رسد که تمرین SIT باوجود حجم تمرینی کم، می­تواند به تعدیل استرس اکسایشی ناشی­از رژیم غذایی پرکالری با نمک ­زیاد در بافت قلب کمک کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Eight-Week Sprint Interval Training (SIT) on Oxidative/Antioxidant Status in Cardiac Tissue of Male Wistar Rats Under A High-Calorie High-Salt Diet

نویسندگان [English]

  • zeinab radaei 1
  • Afsaneh shemshaki 2
  • Ali Samadi 3
1 Master Student of Sports Physiology, Department of Sports Physiology, Faculty of Sport Sciences , Alzahra University, Tehran, Iran.
2 Associate Professor of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran.
3 Assistant Professor of Exercise Physiology, Department of Physical Education and Sport Sciences , Faculty of Humanities, Shahed University, Tehran, Iran.
چکیده [English]

Aim: Consumption of high-calorie, high-salt diets is one of the main factors in production of free radicals and consequent oxidative stress in cardiac tissue. The aim of the present study was to evaluate the effect of an eight-week sprint interval training (SIT) on oxidative/antioxidative status of cardiac tissue in male Wistar rats on a high-calorie, high-salt diet. Methods:  It was an experimental study in which 32 male Wistar rats were randomly divided into four equal groups: normal diet group (ND), normal diet and exercise (ND/SIT), high-calorie high salt diet group (HCL/HS), and high-calorie high salt diet + exercise group (HCL/HS + SIT). The sprint interval training was performed for eight weeks, three times per week, and 4-9 reps per session. Forty-eight hours after the last training session, rats were sacrificed and the cardiac tissue removed and used for superoxide dismutase (SOD), glutathione peroxidase (GPX) activity, as well as total antioxidant capacity (TAC), and malondialdehyde (MDA) levels assessment. Results: Findings showed that eight weeks of SIT significantly increased the activity of SOD and GPX enzymes in the HCL/HS+SIT group. It also significantly reduced the MDA level in cardiac tissue of Wistar rats (P=0.001). But there was no significant change in TAC level after eight weeks of SIT training in the HCL/HS+SIT group (P=0.148). Conclusions: Based on the findings of the present study, it seems that SIT training, despite its low volume, may help in preventing oxidative stress induced by a high-calorie, high-salt diet in the cardiac tissue of Wistar rats.

کلیدواژه‌ها [English]

  • "Oxidative stress"
  • " Sprint Interval Training"
  • "High-salt high-calorie diet"
  • " cardiac tissue"

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 9، شماره 2
مهر 1401
صفحه 112-122

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  • تاریخ دریافت: 14 خرداد 1401
  • تاریخ بازنگری: 18 شهریور 1401
  • تاریخ پذیرش: 30 شهریور 1401

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