تغییرات آپوپتوزی بافت قلب پس از تمرین تناوبی شدید و عسل آویشن در رت‌های دیابتی نوع دو

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I


1 دانشجوی دکتری فیزیولوژی ورزشی، گروه تخصصی تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران.

2 گروه تخصصی تربیت بدنی و علوم ورزشی،دانشگاه آزاد اسلامی،واحد علوم و تحقیقات، تهران، ایران.

3 گروه تخصصی تربیت بدنی و علوم ورزشی،دانشگاه آزاد اسلامی،واحد علوم و تحقیقات، تهران، ایران



هدف: دیابت نوع دو بیماری است که در آن مقاومت انسولینی و بد عملکردی سلول بتا با هم به افزایش غلظت گلوکز خون منجر می­شود و می­تواند باعث آسیب و مرگ سلولی یا آپوپتوز شود. هدف از پژوهش حاضر مطالعه تغییرات آپوپتوزی پروتئین‌های کاسپاز 3 و 9 و سیتوکروم-c  بافت قلب متعاقب تمرین تناوبی شدید و  عسل آویشن  در رت­های دیابتی نوع دو بود. روش شناسی: جامعه آماری را موش­های صحرایی نر تشکیل می­دادند. نمونه­ها شامل 28 سر رت ویستار بودند که پس از 20 هفته تغذیه با رژیم پرچرب با تزریق درون صفاقی ml/kg  25  STZدیابتی شدند و سپس در 4 گروه کنترل دیابتی، تمرین تناوبی، عسل آویشن، تمرین تناوبی- عسل آویشن تقسیم شدند و پروتکل تمرینی و گاواژ عسل روی آنها اجرا شد. پروتکل تمرینی به‌ صورت هشت هفته تمرین تناوبی شدید و پنج جلسه در هفته اجرا شد. انسولین با استفاده از کیت انسولین و به روش الایزا و بیان ژن‌های کاسپاز 3، کاسپاز 9 و سیتوکروم –C توسط روش RT-PCR اندازه‌گیری شد. تحلیل آماری با استفاده نرم‌افزار SPSS نسخه 22 و آزمون تحلیل واریانس دوعاملی و تعیین اندازه اثر و تعقیبی بونفرونی انجام شد. یافته‌ها: در مقایسه با گروه کنترل، تمرین تناوبی شدید به کاهش معنی­دار گلوکز (0001/0) و شاخص مقاومت به انسولین  (001/0) منجر شد. تمرین تناوبی بیان ژن‌های کاسپاز 9 (001/0)، کاسپاز 3 و سیتوکروم c را در سلول‌های قلبی در مقایسه با گروه کنترل افزایش داد. مصرف عسل ­آویشن منجر به کاهش کاسپاز 9 در گروه تعاملی شد. تغییرات کاسپاز 3 و سیتوکروم C در گروهها معنی­دار نبود. نتیجه‌گیری: می‌توان نتیجه گرفت که انجام تمرینات تناوبی شدید توسط بیماران دیابتی می‌تواند منجر به افزایش عامل آپوپتوزی شود. بنابراین استفاده از شدت‌های تعدیل‌یافته­تر و همچنین استفاده از موادی با خواص آنتی‌اکسیدانی و ضدالتهابی مانند عسل در کنار تمرین ورزشی می‌تواند مفید باشد. 



عنوان مقاله [English]

Apoptotic changes of cardiac tissue after HIIT and thyme honey in type 2 diabetic rats

نویسندگان [English]

  • Bahareh Behaein 1
  • Hossein Abednatanzi 2
  • Mandana Gholami 2
  • Farshad GHazalian 3
1 1. Ph.D. Student of Exercise physiology, department of physical education and sport science, Science and research branch, Islamic Azad University, Tehran, Iran.
2 Department of physical education and sport science, Science and research branch, Islamic Azad University, Tehran, Iran
3 Department of physical education and sport science, Science and research branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Aim:   The aim of the present was to Study of apoptotic changes of caspase 3 and 9 proteins and cytochrome c of heart tissue after High intensity interval training (HIIT) and thyme honey in type 2 diabetic rats. Method: The statistical population consisted of male diabetic rats that 20 rats were divided into 4 groups: control, HIIT, thyme honey, HIIT-thyme honey. The training protocol was implemented as eight weeks of HIIT and five sessions per week. Insulin was measured using the insulin kit and the ELISA method and the expression of caspase 3, caspase 9 and cytochrome-C genes by RT-PCR method. Statistical analysis was performed using SPSS version 22 software and two-factor analysis of variance and determination of effect size and Bonferroni post hoc. Results: compared to the control group, HIIT led to a significant decrease in glucose (0.0001) and insulin resistance index (0.001). HIIT increased the expression of caspase 9 genes (0.001) in cardiac cells compared to the control group. Conclusion: It can be concluded that performing HIIT by diabetic patients can lead to an increase in the apoptotic factor. Therefore, the use of more moderated intensities, as well as the use of substances with antioxidant and anti-inflammatory properties, such as honey, along with exercise can be useful.

کلیدواژه‌ها [English]

  • HIIT
  • Type 2 Diabetes
  • Thyme Honey
  • Apoptosis



This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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