تاثیر 8 هفته تمرین تناوبی با شدت بالا در آب بر پروتئین TLR-4 کبد و TNF-α سرم در موش‌های صحرایی نر مبتلا به استئاتوهپاتیت غیرالکلی با رژیم غذایی پرچرب

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشجوی کارشناسی ارشد تغذیه ورزشی، موسسه آموزش عالی زند شیراز، شیراز، ایران

2 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، موسسه آموزش عالی زند شیراز، شیراز، ایران

3 دانشیار فیزیولوژی ورزشی، گروه علوم ورزشی، واحد شیراز، دانشگاه آزاد اسلامی ، شیراز، ایران

4 دانشجوی دکتری فیزیولوژی ورزش، دانشکده علوم‌ تربیتی و روانشناسی، بخش علوم ورزشی، دانشگاه شیراز، شیراز، ایران

چکیده

هدف: هدف از این پژوهش، بررسی تاثیر هشت هفته تمرین تناوبی باشدت بالای (HIIT) شنا بر فاکتور نکروز توموری آلفا (TNF-α) و گیرنده شبه­تول 4 (TLR4) در رت­های مبتلا به استئاتوهپاتیت غیرالکلی (NASH) بود. روش شناسی: در ابتدا موش­های صحرایی با سن 6 تا 8 ماهه به ­طور تصادفی به دو گروه سالم (20= n) و بیمار (رژیم پرچرب (HFD)) (20= n) تقسیم شدند. رژیم پرچرب به مدت 8 هفته ادامه داشت تا موش­های صحرایی مبتلا به NASH شوند. پس از القا بیماری، گروه بیمار به ­طور تصادفی به 2 گروه بیمار- بی­تحرک (10= n)، بیمار- شنا (10= n)،  تقسیم شدند؛ هم­چنین گروه سالم نیز به دو گروه سالم- بی­تحرک (10= n) و گروه سالم- شنا (10= n) تقسیم گردیدند. تمرین HIIT شنا، شامل 14 نوبت 20 ثانیه­ای شنا با 10 ثانیه استراحت بین هر نوبت (سه روز در هفته به مدت هشت هفته) بود. در تمرین تناوبی بار اعمال شده در هفته اول، وزنه­ای به میزان 7 درصد وزن بدن هر موش صحرایی بود و هر هفته 1 درصد به آن اضافه شد. پروتئین TLR4 از بافت کبد و از طریق تکنیک وسترن بلات و TNF-α از سرم خون اندازه­گیری شدند. برای مشخص نمودن تفاوت میان گروه­ها از آزمون آماری آنووای یک راهه و آزمون تعقیبی بونفرونی (05/0p<) استفاده شد. یافته‌ها: نتایج آنالیز آماری نشان داد در گروه­های سالم-شنا و بیمار-شنا نسبت به بیمار بی­تحرک در متغیر TNF-α کاهش معنادار  (001/0P=) داشت و در متغیر TLR4 در گروه سالم-شنا نسبت به بیمار بی­تحرک (04/0P=) کاهش معنادار و در گروه بیمار-شنا نسبت به بیمار بی­تحرک (87/0P=) کاهش غیرمعنادار داشتند. نتیجه‌گیری: این نوع تمرینات در بیماران مبتلا به NASH بر متغیر TNF-α نسبت به TLR-4 اثر کاهش بیشتری داشته است و در مجموع می­توان گفت تمرینات HIIT شنا بر میزان سطح التهاب کبد در بیماران NASH مفید است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of 8 weeks of high-intensity interval water training on liver TLR-4 protein and serum TNF-α in male rats with non-alcoholic steatohepatitis by high-fat diet

نویسندگان [English]

  • Mohadese Sayyad 1
  • Homa Sheikhani 2
  • Mehrzad Moqaddasi 3
  • Alireza Jowhari 4
1 Ms student of sports nutrionion,Zand Higher Education Institute, shiraz, Iran.
2 Assistant professor of sport physiology, Department of sport Science, Zand Higher Education Institute, shiraz, Iran.
3 Associate professor in exercise physiology, Shiraz branch, Islamic Azad university, shiraz, Iran
4 PhD Student in Exercise Physiology, Faculty of Educational Sciences and Psychology, Department of Sports Sciences, Shiraz University, Shiraz, Iran
چکیده [English]

Aim: The aim of this study was to evaluate the effect of eight weeks of high-intensity intermittent exercise (HIIT) swimming on TNF-α and TLR4 in rats with nonalcoholic steatohepatitis (NASH). Methods: rats aged 6 to 8 years were randomly divided into healthy (n = 20) and patient (high fat diet (HFD)) (n=20) groups. The HFD lasted for 8 weeks until the rats became infected with NASH. After induction of the disease, the patient group was randomly divided into 2 groups: patient- inactive (n=10), patient-swimming (n=10); Also, the healthy group was divided into two groups: healthy-inactive (n=10) and healthy-swimming group (n=10). The HIIT swimming exercise consisted of 14 20-second swimming sessions with a 10-second break between each session (three days a week for eight weeks). In the intermittent exercise of the load in the first week, the weight was 7% of the body weight of each rat and 1% was added to it every week. TLR4 protein was measured from liver tissue by Western blotting and TNF-α from serum. One-way ANOVA and Bonferroni post hoc test (p <0.05) were used to determine the differences between the groups. Results: in healthy-swimming and patient-swimming groups there was a significant decrease in the TNF-α variable compared to the sedentary patient (P=0.001) and in the TLR4 variable in the healthy-swimming group relative to the patient. There was a significant decrease in  inactive (P=0.04) and a non-significant decrease in the patient-swimming group compared to the sedentary patient (P=0.87). Conclusion: This type of exercise in patients with NASH had a greater effect on TNF-α than TLR-4 and in general, it can be said that HIIT swimming training are useful on the level of liver inflammation in NASH patients.
 

کلیدواژه‌ها [English]

  • High intensity interval Swimming training
  • Non-Alcoholic steatohepatitis
  • TNF-α
  • TLR4

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 9، شماره 1
فروردین 1401
صفحه 125-135

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  • تاریخ دریافت: 24 فروردین 1401
  • تاریخ بازنگری: 28 اردیبهشت 1401
  • تاریخ پذیرش: 31 اردیبهشت 1401

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