تاثیر تمرین تناوبی شدید بر بیان ژن پروتئین شوک گرمایی 70 و کاسپاز 3 کاردیومیوسیت‌های موش‌های نر مدل سکته قلبی.

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزش، گروه تربیت بدنی، دانشکده علوم تربیتی و روان شناسی، اردبیل، ایران

2 استادیار، گروه فیزیولوژی ورزش، دانشکده علوم تربیتی و روان شناسی، دانشگاه محقق اردبیلی، اردبیل، ایران..

3 دانشیار، گروه فیزیولوژی ورزش، دانشکده علوم تربیتی و روان‌شناسی، دانشگاه محقق اردبیلی، اردبیل، ایران.

چکیده

هدف: هدف پژوهش بررسی تاثیر تمرین هوازی تناوبی شدید بر بیان ژن پروتئین شوک گرمایی 70 و کاسپاز 3 کاردیومیوسیت‌های موش‌های نر مدل سکته قلبی بود. روش شناسی: تعداد 30 سر موش صحرائی نر 16 هفته‌ای با وزن 200 تا 250 گرم نژاد ویستار با تزریق درون صفاقی ایزوپروترنول (با دوز 100 میلی‌گرم بر هر کیلوگرم وزن بدن در دو روز متوالی) دچار آنفارکتوس میوکارد (تایید از طریق شاخص‌های آسیب قلبی: CK، LDH و CTnI) شدند و به طور تصادفی در سه گروه (10 سر موش در هر گروه) کنترل سالم، کنترل سکته، سکته + تمرین (HIIT+MI) تقسیم شدند. تمرین HIIT به مدت 8 هفته (5 روز در هفته) شامل 60 دقیقه دویدن تناوبی(4 دقیقه دویدن با شدت 90-85 درصد VO2max و 2 دقیقه ریکاوری فعال با شدت 60 – 50 درصد) انجام شد. بیان ژن‌های HSP70 و کاسپاز 3 کاردیومیوسیت‌ها با استفاده از روش Real-Time PCR انجام شد. داده‌ها با استفاده از روش تحلیل واریانس یک طرفه و آزمون تعقیبی توکی در سطح معنی‌داری 05/0 >  pتحلیل شدند. یافته‌ها: بیان HSP70 کاردیومیوسیت‌ها در موش‌های کنترل مدل MI (سکته کنترل) نسبت به گروه سالم کنترل کمتر بود (001/0=p)؛ با این حال، بیان HSP70 در گروه تمرین HIIT به طور معنی‌داری بیشتر از گروه سکته کنترل و کنترل سالم بود (به ترتیب 001/0=p، 001/0=p). همچنین، بیان کاسپاز 3 در گروه تمرین HIIT به طور معنی داری کمتر از گروه‌های کنترل سالم و کنترل سکته بود (به ترتیب 001/0=p، 001/0=p). نتیجه‌گیری: به نظر می‌رسد تمرین تناوبی شدید در مهار آپوپتوز قلبی ناشی ناشی از سکته موثر باشد. اما، به دلیل محدودیت‌های تحقیق و کمبود شواهد، هنوز نیاز به تحقیقات بیشتر باقی است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of high intensity interval training on cardiomyocytes HSP70 and Caspase-3 gene expression levels in myocardial infarction male rat model.

نویسندگان [English]

  • Gabrial Pouzesh Jadidi 1
  • Farnaz Seify 2
  • Lotfali Bolboli 3
  • Amaneh Pourrahim 3
1 PhD Student of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
2 Assistant Professor of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
3 Associate Professor of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
چکیده [English]

Aim: The aim of this study was to investigate the effect of high intensity interval training on cardiomyocytes HSP70 and Caspase-3 gene expression levels in myocardial infarction (MI) male rat model.
 Methods: Thirty male Wistar rats (age: 16 wks, weight: 200 to 250 grams) were randomly divided into three groups of healthy control, MI control and MI training following induction of MI (verified by CK, LDH and CTnI as cardiac injury indices) via intraperitoneal injection of isoproterenol (100 mg/kg.day) within two consecutive days. HIIT was included on eight weeks (5 sessions per week), each session was consisted of 10 bouts of running occasions (each for 4 min) at 85 - 90% of vVo2max with 2 min rest intervals at 50 to 60% of vVO2max. The gene expression levels of HSP70 and Caspase-3 were evaluated using Real-Time PCR method and the data were analyzed using one-way analysis of variance and Tukey post hoc test at the significance level of p <0.05.
Results: Cardiomyocytes HSP70 expression levels was lower in MI control than healthy control counterparts (p=0.001); However, in the HIIT group the expression levels of HSP70 and caspase-3 genes were respectively higher and lower compared to both of healthy control and MI control groups (p=0.001, p=0.001 respectively).
Conclusions:  It seems that HIIT could be efficient for inhibition of MI induced cardiac apoptosis. However further research is needed in this area because of study limitations and lack of similar evidence.

کلیدواژه‌ها [English]

  • HIIT
  • HSP70
  • Myocardial infarction
  • Caspase-3

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 9، شماره 2
مهر 1401
صفحه 1-11

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  • تاریخ دریافت: 25 آبان 1400
  • تاریخ بازنگری: 11 آذر 1400
  • تاریخ پذیرش: 21 آذر 1400

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