اثر ترکیبی تمرین هوازی با عصاره زعفران بر برخی فاکتورهای تنظیمی آپوپتوز عضله نعلی موش های صحرایی نر متعاقب فعالیت وامانده ساز هوازی

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسنده

استادیار، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه آیت الله العظمی بروجردی (ره) ، بروجرد، ایران

چکیده

هدف: زعفران سرشار از ترکیبات آنتی اکسیدانی بوده که قادر به بلوکه کردن آپوپتوز ناشی از فعالیت های ورزشی وامانده ساز می باشد. تحقیق حاضر به منظور تعیین اثر ترکیبی تمرین هوازی با عصاره زعفران بر برخی فاکتورهای تنظیمی آپوپتوز عضله نعلی موش های صحرایی نر متعاقب فعالیت وامانده ساز هوازی انجام شد. روش شناسی: در این مطالعة تجربی، 48 سر رت نر نژاد ویستار به‌صورت تصادفی به چهار گروه مساوی: G1؛ دارونما (بدون تمرین)، G2؛ مکمل (بدون تمرین+ 100 میلی گرم عصاره آبی زعفران)، G3 ؛ تمرین ( 8 هفته تمرین استقامتی)، و G4؛ ترکیبی ( 8 هفته تمرین استقامتی + 100 میلی گرم عصاره آبی زعفران) تقسیم شدند. در پایان تحقیق نیمی از موش‌ها بلافاصله پیش و نیمی‌دیگر بلافاصله پس از وامانده شدن بر روی نوار گردان، قربانی شدند. بیان ژن هایbax  و bcl-2با استفاده از روش Real-time PCR سنجیده شدند. داده‌ها با استفاده از روش آماری تحلیل واریانس یک‌طرفه، در سطح معناداری 05/0 تجزیه ‌و تحلیل شدند. یافته ها: نتایج نشان داد پیش از وامانده سازی بیان پروتئین bcl-2 و نسبتBax  به  Bcl-2گروه های G3 و G4 به طور معنی داری به ترتیب بیشتر و کمتر از دیگر گروه ها بود (05/0p <). همچنین متعاقب وامانده‌سازی بیان پروتئین های Bax و Bcl-2 عضله نعلی موش های تمامی گروه ها بجز گروه G4 به ترتیب به طور معنی داری افزایش و کاهش نشان داد در حالی که نسبت  Baxبه Bcl-2 افزایش یافت (05/0p <). نتیجه گیری: در کل هشت هفته تمرین هوازی به تنهایی و با ترکیب با مصرف عصاره زعفران موجب افزایش معنی دار بیان پروتئین bcl-2 عضله نعلی موش ها می شود. همچنین تمرین هوازی توام با مصرف عصاره زعفران از افزایش بیان پروتئین Bax و نسبت Bax به Bcl-2 متعاقب یک وهله فعالیت وامانده ساز جلوگیری می کند.

کلیدواژه‌ها


عنوان مقاله [English]

The Combined Effect of Aerobic Exercise with Saffron Extract on Some Indices of Soleus Muscle Apoptosis Regulatory Factors of Male Rats Following an Aerobic Exercise Until Exhaustion

نویسنده [English]

  • Amir Khosravi
Assistant Professor، Department of Physical Education and Exercise Science، Faculty of Humanities Ayatollah Ozma Borujerdi University, Borujerd، Iran
چکیده [English]

Aim: Saffron has high levels of antioxidant compounds are able to Inhibition of apoptosis induced by exercise to exhaustion. The present study aims to investigate the effect of the combined effect of aerobic exercise with Saffron extract on some indices of soleus muscle apoptosis regulatory factors of male rats following an aerobic exercise until exhaustion. Methods: 48 Wistar male rats were assigned into following four groups (N=10): G1) placebo; without exercise: G2) supplement; without exercise+ Saffron extract, 100 mg/kg :G3) exercise; 8 weeks exercise: G4) combination; 8 weeks exercise + Saffron extract, 100 mg/kg. The end of experiment, half of the rats was killed immediately before exhaustive exercise; on the other hand, remaining rats were killed immediately after performing an acute bout of exhaustive exercise on the treadmill. Bax and Bcl2 genes expression were analyzed through the Real Time-Polymerase Chain Reaction (RT-PCR). The data were analyzed using a one-way ANOVA, with the significance level of P < 0.05. Results: The results showed that prior exhaustive bcl-2 gene expression and Bax/Bcl2 ratio of the G3 and G4r groups were significantly mor and lower than the other groups respectively (P < 0.05). As well as, in the all groups except G4, the bax and bcl-2 protein expression increased and decreased, respectively while bax/bcl-2 ratio increased following an exhausting exercise (P < 0.05). Conclusion: In general, 8 weeks aerobic training only and combinational, with use of saffron extract significant increase bcl-2 expression in soleus muscle of rats. As well as the use of saffron extract with aerobic exercise prevented the increase of Bax gene expression and Bcl-2/Bax ratio following an exhausting exercise.

کلیدواژه‌ها [English]

  • Aerobic Exercise Until Exhaustion
  • Apoptosis
  • Soleus Muscle
  • Saffron Extract
 
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