اثر فعالیت راه رفتن با محدودیت جریان خون بر مسیر سیگنالی mTOR عضله اسکلتی مردان تمرین نکرده

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران.

2 استاد گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران.

چکیده

هدف: هدف از این مطالعه بررسی اثر فعالیت راه رفتن با محدودیت جریان خون (BFR) بر فعالیت مسیر سیگنالی mTOR عضله اسکلتی مردان تمرین نکرده بود. روش شناسی: پنج مرد سالم تمرین نکرده در دو وهله جداگانه (به فاصله سه هفته) مورد مطالعه قرار گرفتند: (1) راه‌رفتن با BFR بر روی تردمیل با شدت 40% از توان هوازی بیشینه (VO2max) و (2) فعالیت در شرایط مشابه بدون BFR. نمونه‌های بایوپسی (پهن جانبی) و نمونه‌های خونی (سیاهرگ بازویی) قبل و بعد از فعالیت در فواصل زمانی معین جهت بررسی میزان فسفوریلاسیون پروتئین و تغییرات در هورمون رشد (GH)، فاکتور رشد شبه انسولینی (IGF-1) و لاکتات خون گرفته شد. یافته ها: نتایج نشان داد که فسفوریلاسیون پروتئینهای  mTORو p70S6K در مقایسه با مقادیر قبل از فعالیت در هیچ یک از دو گروه BFR و کنترل تغییر معناداری نداشت (05/0p ˃). IGF-1، بلافاصله بعداز فعالیت در مقایسه با مقادیر قبل از فعالیت افزایش معنادار نشان داد (05/0p ˂). GH بلافاصله بعد از فعالیت در مقایسه با مقادیر قبل از فعالیت و در مقایسه با گروه کنترل افزایش معنادار نشان داد (05/0 ˂ p ). لاکتات خون در هیچ یک از گروه ها تغییر معناداری نداشت (05/0 ˃ p ). نتیجه گیری: نتایج مطالعه حاضر نشان داد که فسفوریلاسیون پروتئینهای mTOR و S6K به همراه فعالیت راه رفتن با محدودیت جریان خون تحریک نمی شود. در نتیجه مسیر سیگنالی mTOR به عنوان یک مکانیسم احتمالی نقش چندانی در افزایش سنتز پروتئین به دنبال فعالیت راه رفتن با BFR ندارد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Walking Exercise with Blood Flow Restriction On mTOR Signaling Pathway in Untrained Male Subjects’ Skeletal Muscle

نویسندگان [English]

  • saeid hallajbashi 1
  • Bahman Mirzaei 2
  • Farhad Rahmaniniya 2
1 PhD Student of Sport Physiology, Department of Sport Physiology, Faculty of Physical Education and Sports Sciences , Rasht, Iran.
2 Professor of Sports Physiology Department, Faculty of Physical Education and Sports Sciences, Gilan University, Rasht, Iran.
چکیده [English]

Aim: The purpose of this study was to study the acute responses to BFR walking exercise on the mTOR signaling pathway in untrained male subjects. Methods: five (n=5) apparently healthy untrained male participants were studied identically on two occasions: (1) BFR aerobic exercise consisting of walking at an exercise intensity of 40% VO2max (2) Identical walking exercise without BFR (Ctrl). Each trial was separated by 3 weeks. Muscle biopsy (vastus lateralis) and blood sample (antecubital vein) were obtained pre- and post-exercise in given time points to determine protein expression and changes in GH and IGF-1. Results: Results showed that mTOR and p70S6K phosphorylation levels did not change significantly in both groups (P ˃ 0.05). IGF-1 and GH immediately after exercise increased in BFR group (P˂0.05). Blood lactate did not show any significant change in both groups. Conclusion: We find that the phosphorylation of p70S6K and mTOR did not activate after a bout of walking with blood flow restriction. As result the mTOR signaling pathway is not responsible for stimulating skeletal muscle protein synthesis after BFR walking exercise.

کلیدواژه‌ها [English]

  • Blood Lactate
  • Kaatsu Training
  • Muscle Biopsy
  • Muscle Hypertrophy

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 6، شماره 2
مهر 1398
صفحه 9-16

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سابقه مقاله

  • تاریخ دریافت: 19 آبان 1398
  • تاریخ بازنگری: 23 دی 1398
  • تاریخ پذیرش: 29 بهمن 1398

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