اثر چهار هفته تمرین مقاومتی برسطوح پروتئین ABCA1 کبدی و سطوح HDL-C پلاسمایی موشهای نر ویستار

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 کارشناسی ارشد فیزیولوژی ورزشی، گروه تربیت بدنی آموزش و پرورش، شهر زنجان، زنجان، ایران.

2 استادیار فیزیولوژی ورزشی، گروه تربیت بدنی، دانشگاه بناب، بناب، ایران.

چکیده

هدف: ABCA1 عضوی از خانواده ناقل‌های‌ غشایی ABC در پستانداران است که نقشی اساسی در نوآراییHDL-C و پیشگیری از بیماری آترواسکلروزیس (تصلب شرائین) دارد. هدف از پژوهش بررسی تغییرات ABCA1 کبدی و سطوح پلاسمایی HDL-C موش‌های نر ویستار به دنبال چهار هفته تمرین مقاومتی است. روش شناسی: 16موش صحرایی نر ویستار 14-12 هفته‌ای با میانگین وزن290 گرم در دو گروه کنترل (8n=) و تمرین (8n=) در این پژوهش مورد استفاده قرار گرفت. تمرین مقاومتی شامل بالا رفتن از یک نردبان یک متری به همراه وزنه بسته شده به دم حیوانات بود. موش ها 48 ساعت بعد از آخرین جلسه تمرینی قربانی شد. بافت کبد نمونه برداری شده فوراً  پس از شستشو  با سرم فیزیولوژیک توسط نیتروژن مایع فریز، و به یخچال با دمای C 70- انتقال یافت. پلاسما نیز جهت اندازه‌گیری HDL وapoA-I  (آپولیپوپروتئین اصلی HDL) از نمونه خونی که به طور مستقیم از قلب گرفته شده بود، جداسازی شد. برای بررسی میزان پروتئین  ABCA1و apoA-I  از کیت الایزا و برای اندازه­گیری سطح HDL از روش ایمنی آنزیمی استفاده شد. یافته‌ها: نتیجه نشان داد که میزان  ABCA1در کبد موش‌های تمرین کرده حدود 25 درصد بالاتر از گروه کنترل بود، اما این اختلاف از نظر آماری معنی‌دار نبود (05/0<P). به­علاوه سطوح HDL وapoA-I با وجود مقداری افزایش از نظر آماری معنا دار نبود (05/0<P). با وجود چهار هفته‌ تمرین مقاومتی از نظر آماری تغییر معنی‌داری در فاکتور‌های اصلی ایجاد نشد (05/0<P). نتیجه‌گیری: اما با توجه به روند داده‌ها به نظر می‌رسد این پروتکل تمرین تاحدودی مؤثر بوده‌است. احتمالاً با افزایش شدت و طول تمرینات و یا طراحی تمرینات ترکیبی نتایج بهتری حاصل شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of 4 Week Resistance Training on Male Rat Hepatic ABCA1 Protein Plasma HDL-C Levels

نویسندگان [English]

  • Leila Ganjkhani 1
  • Ali Osali 2
1 Msc of Exercise Physiology, Department of Physical Education, Zanjan, Zanjan,Tehran.
2 Assistant Professor of Exercise Physiology, Department of Physical Education, University of Bonab , Bonab, Iran.
چکیده [English]

 Background and aim:ABCA1, a member of ABC (ATP-binding cassette transporters) family in mammals plays a fundamental role in HDL-C remodeling and thus prevent atherosclerosis. The purpose of this study was to investigate the changes of hepatic ABCA1 and plasma of HDL-C levels of male Wistar rats following four weeks of resistance training. Methods: 16 male Wistar rats with the age of 12-14 weeks and a mean body weight of 290± 10g were used. Rats were divided in Two groups of control (n=8) and exercise (n=8). Resistance training consisted of climbing a one meter ladder (26 stairs and 80 degree angle with the ground) with weights tied to the tail of the animal. Finally, rats performed three consecutive sets with 100% of their body weights (6 reps per set). Rats were sacrificed 48 h after the last training session. Sampled Liver tissue, after washing with saline, were immediately frozen by liquid nitrogen. Finally, samples were transferred to -70°C. Blood samples were directly taken from the heart and the plasma were isolated for measurement of the HDL and apoA-I (the major apolipoprotein of HDL).  ELISA kit was used to evaluate the amount of ABCA1 and apoA-I and Plasma HDL level was determined by enzymatic immune method. For data analysis, independent T test was used. Results: The results showed that ABCA1 in the liver of rats was higher for about 25 percent higher than the control group, but was not statistically significa (P>0/05). Plasma HDL levels were not significantly different between two groups (P>0/05). Moreover, despite some increases in apoA-I levels they were not statistically significant (P>0/05).  Conclusions: Thus, despite the fact that four weeks of resistance training didn’t make statistically significant changes in ABCA1 and HDL and apoA-I, but according to the trend of the data this resistance training protocol seemed to be partly effective Possibly, better results can be achieved by increasing the intensity and leng of the execises, or by designing a beter combination of exercises.

کلیدواژه‌ها [English]

  • ABCA1
  • Resistance Training
  • HDL-C and ApoA-I

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مطالعات کاربردی تندرستی در فیزیولوژی ورزش
دوره 6، شماره 1
فروردین 1398
صفحه 22-30

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سابقه مقاله

  • تاریخ دریافت: 29 خرداد 1398
  • تاریخ بازنگری: 06 شهریور 1398
  • تاریخ پذیرش: 22 شهریور 1398

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