Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license
Authors
1
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran.
2
Department of Exercise Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
3
Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran.
10.22049/jahssp.2026.31423.1810
Abstract
Abstract
Background & Purpose: Type 2 diabetes (T2D) is a metabolic disorder that can impair kidney function. This study aimed to investigate the interactive effects of moderate-intensity continuous aerobic training (MICT) and vitamin D₃ on Kidney function in T2D rats. Methods: Forty male Wistar rats (8 weeks old, 250–300 g) after an acclimatization, were divided into a standard diet group (n=8) and a high-fat diet group (n=32). T2D was induced in the high-fat diet group by 6 weeks of high-fat feeding followed by a single injection of streptozotocin. Subsequently, rats were divided into five groups of eight: Healthy Control, Diabetic Control, Diabetic with Aerobic Training, Diabetic with Vitamin D₃ injection, and Diabetic with combined Aerobic Training and Vitamin D₃. The MICT protocol consisted of treadmill running for 30–60 minutes per session at 55–65% of maximal running speed for 8 weeks, and Vitamin D₃ was administered subcutaneously at a dose of 10,000 IU/kg once weekly. At the end of the intervention, serum glucose, insulin, and Kidney proteins sFas and sFasL were measured. Results: In the Diabetic Control group, compared to the Healthy Control group, serum sFas and the sFas/sFasL ratio were significantly higher, while sFasL were lower (P < 0.001), indicating excessive apoptosis activation. Furthermore, following 8 weeks of MICT, the training groups exhibited significantly reduced sFas and sFas/sFasL ratio, and increased sFasL compared to the Diabetic Control group. The greatest reduction in apoptosis was observed in the combined intervention group (P < 0.001). Additionally, these interventions effectively modulated serum insulin, glucose levels, and the HOMA-IR index. Conclusions: T2D promotes apoptosis in Kidney tissue. The combination of MICT and vitamin D₃ improved Kidney function and reduced apoptosis in diabetic rats. These findings suggest that integrating these two interventions may represent an effective strategy to mitigate diabetes-induced Kidney complications.
Keywords
Main Subjects
)