Effect of Combined Aerobic and Resistance Exercise Training on Joint Levels of Some Inflammatory Biomarkers in Aged Female Rats

Articles in Press

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of physical education, Boj.C., Islamic Azad University, Bojnourd, Iran

2 Department of physical education, Bojnourd .C., Islamic Azad University, Bojnourd, Iran

3 Department of Physical Education, Farhangian University, Tehran, Iran

Abstract

Osteoarthritis (OA) is a multifactorial inflammatory disorder in which inflammatory biomarkers play a key pathogenic role. Given the anti-inflammatory effects of exercise training, the purpose of the present study was to investigate the effect of eight weeks of combined aerobic and resistance training on the levels of the P2X7 purinergic receptor (P2RX7)and nucleotide-binding oligomerization domain- like receptor protein 3(NLRP3) in the synovial fluid and articular cartilage of aged female rats.Twenty-two Wistar rats (24–28 months, 262.5 ± 15.10 g) were randomly divided into two groups: experimental and control (n= 11 per group). Rats in the experimental group participated in eight weeks of combined aerobic (running on treadmill at an intensity of 40–60% of maximum velocity, 60 minutes per session, without treadmill incline) and resistance training (15 times of climbing on rodent ladder at intensity of 40-60 percentage of maximum workload with 1 minute of rest interval, 45 minutes per session), on alternating days and five days a week. All rats were dissected 48 hours after the last training session, and the measured variables were evaluated using appropriate laboratory methods. Data were analyzed using independent-sample and paired-sample t-tests, with a significance level set at P < 0.05. Eight weeks of combined training led to significant reductions in the experimental group compared to the control group in the following measures: synovial fluid levels of P2RX7 and NLRP3 , cartilage levels of NLRP3 , and body weight. Furthermore, body weight significantly decreased in the experimental group in the after training compared to the before training. It appears that eight weeks of combined aerobic and resistance training can be effective in improving improving OA-related inflammatory status in aged rats by reducing P2RX7 and NLRP3 levels. However, to clarify the mediating mechanisms underlying these effects, it is recommended to measure related inflammatory cytokines in future research.

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Journal of Applied Health Studies in Sport Physiology

Articles in Press, Accepted Manuscript
Available Online from 29 January 2026

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History

  • Receive Date: 17 May 2025
  • Revise Date: 05 January 2026
  • Accept Date: 19 January 2026

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