The effect of a period of aerobic training on the levels of mitochondrial proteins FIS1 and MFN1 in muscle tissue of diabetic male Wistar rats

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Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 department of physical education, islamic azad university ahvaz branch, ahvaz, iran

2 Department of Physical Education, Ahv.C., Islamic Azad University, Ahvaz, Iran.

Abstract

Aim: Impaired mitochondrial function in skeletal muscles is often associated with many metabolic diseases such as diabetes. Therefore, the aim of the present study was to investigate the effect of eight weeks of aerobic training on the levels of Mfn1 and FIS1 proteins in the gastrocnemius muscle tissue of diabetic rats. Methods: 18 male Wistar rats, eight weeks old and weighing 220±15 g, were randomly assigned to three groups: healthy control, diabetic control, and diabetic training. Type 2 diabetes was induced by a combination of a high-fat diet and streptozotocin injection. the aerobic training program was carried out for eight weeks based on the principle of gradual overload from 15 m/min for 30 minutes in the first week to 25 m/min for 60 minutes in the eighth week. 48 hours after the last training session, the gastrocnemius muscle tissue was extracted and stored at -80°C. Mfn1 and FIS1 protein levels were measured by Western blotting. One-way analysis of variance (ANOVA) with Tukey's post hoc test at a significance level of 0.05 was used for statistical analysis SPSS-23 software. Results: Diabetes induced by a combination of a high-fat diet and streptozotocin injection resulted in a significant decrease in Mfn1 protein and a significant increase in FIS1 protein in the gastrocnemius muscle tissue of diabetic mice (P < 0.05). However, eight weeks of aerobic training resulted in a significant increase in Mfn1 protein and a significant decrease in FIS1 protein in the gastrocnemius muscle tissue of diabetic mice (P < 0.05).Conclusion: According to the results of the present study, it is concluded that physical activity should be considered as a useful and non-pharmacological strategy to maintain mitochondrial dynamics and, consequently, its optimal function in skeletal muscles in diabetes.

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Journal of Applied Health Studies in Sport Physiology

Articles in Press, Accepted Manuscript
Available Online from 08 December 2025

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History

  • Receive Date: 27 August 2025
  • Revise Date: 30 November 2025
  • Accept Date: 03 December 2025

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