Effect of Aerobic Interval Training on the Expression of Cardiac A20 and CYLD Proteins in a Rat Model of Myocardial Infarction

Articles in Press

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Exercise Physiology, University of Mohaghegh Ardabili, Ardabil, Iran

2 Associate Professor of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran

3 Associate Professor of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.

4 Assistant Professor of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran

10.22049/jahssp.2025.30394.1723

Abstract

Myocardial ischemia/reperfusion injury (MI/RI) is one of the determining factors of cardiovascular disease disorders, which is associated with cell death caused by inflammation and impaired activity of deubiquitinating proteins. The aim of the present study was to determine the effect of Interval aerobic training on the expression of A20 and CYLD proteins in the heart tissue of male myocardial infarction model mice.In this experimental study, 30 16-week-old male Wistar rats were randomly divided into three groups: control (healthy), myocardial infarction (MI), and interval training. Myocardial infarction was performed by direct intervention with LAD occlusion for 30 minutes. Interval protocol with moderate intensity of 60 minutes of intermittent running on a treadmill, each interval including 4 minutes of running with an intensity of 65-70% of VO2max and 2 minutes of active recovery with an intensity of 50-60% of VO2max, three days a week and for 8 weeks. Data were compared using one-way analysis of variance and Tukey's post hoc test at a significance level of p<0.05. Result: The result of Tukey's post hoc test for the A20 variable showed that the expression level of this protein in the MI group was significantly lower than that of the healthy control group. Also, the expression level of A20 protein in the interval training+MI group was significantly increased compared to the MI group and the healthy control group. The results showed that although the expression level of CYLD protein in the MI group was reduced compared to the healthy control group; but it was not statistically significant, the expression level of CYLD protein in the interval training+MI group was increased compared to the healthy control and MI groups. Conclusion: Interval aerobic training may prevent inflammation-induced cell death by increasing the expression of cardiac A20 and CYLD proteins.

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Journal of Applied Health Studies in Sport Physiology

Articles in Press, Accepted Manuscript
Available Online from 20 May 2025

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History

  • Receive Date: 04 March 2025
  • Revise Date: 18 May 2025
  • Accept Date: 20 May 2025

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