The effect of high-intensity interval training and intermittent fasting on lipolysis markers in liver tissue of rats fed a high-fat and high-carbohydrate diet

Articles in Press

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran

2 Department of Sports Science, Faculty of Humanities, University of Kashan, Kashan, Iran

10.22049/jahssp.2025.30212.1709

Abstract

Background& purpose: Consumption of high-fat and high-carbohydrate diets causes metabolic disorders in the liver. In contrast, exercise and nutritional interventions are effective strategies to improve the destructive effects of these types of diets. The aim of the present study was to investigate the effect of high-intensity interval training and intermittent fasting on markers of lipolysis in liver tissue of rats fed a high-fat and high-carbohydrate diet. Methods: Thirty Sprague-Dawley male rats with 8-week-old were randomly divided into standard diet (ND) groups (6), high-fat, high-carbohydrate diet (HFD+HC) (6), high-fat, high-carbohydrate diet plus exercise (HFD+HC+HIIT) (6), high-fat, high-carbohydrate diet plus intermittent fasting (HFD+HC+ADF) (6), and high-fat, high-carbohydrate diet plus exercise and intermittent fasting (HFD+HC+HIIT+ADF) (6). The rats underwent 12 weeks of interventions that included high-intensity interval training (HIIT) 5 sessions per week and intermittent fasting (ADF) consisting of one-day periods between eating and fasting. CPT1 and PPARα protein levels were measured using Western blotting, and data were analyzed using one-way ANOVA and Tukey post hoc tests at a significance level (P≤0.05). Results: The results showed that high-fat, high-carbohydrate diet significantly decreased the protein levels of PPARα and CPT1 in liver tissue of HFD+HC compared to ND group (P<0.05). However, HIIT significantly increased the protein levels of CPT1 in liver tissue compared to HFD+HC group (P=0.04). Also, the combination of HIIT and ADF significantly increased the protein levels of PPARα and CPT1 in liver tissue compared to HFD+HC group (P≥0.05). Conclusions: HIIT and ADF simultaneously had a significant effect on the expression of PPARα and CPT1 and probably prevent liver disorders caused by high-fat diets by improving lipid metabolism.

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Journal of Applied Health Studies in Sport Physiology

Articles in Press, Accepted Manuscript
Available Online from 18 April 2025

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History

  • Receive Date: 25 December 2024
  • Revise Date: 29 March 2025
  • Accept Date: 21 March 2025

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